171113
Which of the following is not a component of downstream processing?
1 Separation
2 Purification
3 Preservation
4 Expression
Explanation:
Expression is not a component of downstream processing. Downstream processing involves the separation and purification of products synthesized in bioreactors. After completion of the process, the products formed are preserved for future use.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171114
Stirred-tank bioreactors have been designed for
1 purification of product
2 addition of preservatives to the product
3 availability of oxygen throughout the process
4 ensuring anaerobic conditions in the culture vessel
Explanation:
In the stirred - tank bioreactor, the stirrer facilitates even mixing and oxygen availability throughout the bioreactor. - Stirred tank bioreactors have several advantages over shake flasks - (i) Small volumes of culture can be taken out from the rector for sampling or testing. (ii) It has a foam breaker for regulating the foam. (iii) It has a control system that regulates the temperature and $\mathrm{PH}$.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171116
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 Ligation
2 Annealin
3 Extension
4 Denaturation
Explanation:
High temperature about $94^{\circ} \mathrm{C}$ is required for the process of denaturation which is the first step of PCR. - Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase. - Annealing is performed at $50^{\circ}-60^{\circ} \mathrm{C}$ which is the second step that can get affected.
NEET-2021
Biotechnology : Principles and Processes
171117
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
1 Polysaccharides
2 RNA
3 DNA
4 Histones
Explanation:
Various enzymes like protease RNAse, etc are added to breakdown substances like proteins, RNA, etc. - All these substances are broken down, DNA is left which is precipitated out by adding chilled ethanol. - Histones are special proteins that help condense DNA in a cell.
NEET-2021
Biotechnology : Principles and Processes
171118
Which statement is correct for bacterial transduction : -
1 Transfer of some genes from one bacteria to another bacteria through virus
2 Transfer of some genes from one bacteria to another bacteria by conjugation
3 Bacteria obtained its DNA directly
4 Bacteria obtained DNA from other external source
Explanation:
In transduction, genetic material from one bacteria to another by virus (bacteriophage). It was discovered by Joshua Lederberg and Norton Zinder while working on the recombination of genes in bacteria and this technique of transfer virul DNA and applied to the bacterium Salmonella typhimurium. - Transduction is used for gene mapping \& analysis in bacteria and also for strain construction.
171113
Which of the following is not a component of downstream processing?
1 Separation
2 Purification
3 Preservation
4 Expression
Explanation:
Expression is not a component of downstream processing. Downstream processing involves the separation and purification of products synthesized in bioreactors. After completion of the process, the products formed are preserved for future use.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171114
Stirred-tank bioreactors have been designed for
1 purification of product
2 addition of preservatives to the product
3 availability of oxygen throughout the process
4 ensuring anaerobic conditions in the culture vessel
Explanation:
In the stirred - tank bioreactor, the stirrer facilitates even mixing and oxygen availability throughout the bioreactor. - Stirred tank bioreactors have several advantages over shake flasks - (i) Small volumes of culture can be taken out from the rector for sampling or testing. (ii) It has a foam breaker for regulating the foam. (iii) It has a control system that regulates the temperature and $\mathrm{PH}$.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171116
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 Ligation
2 Annealin
3 Extension
4 Denaturation
Explanation:
High temperature about $94^{\circ} \mathrm{C}$ is required for the process of denaturation which is the first step of PCR. - Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase. - Annealing is performed at $50^{\circ}-60^{\circ} \mathrm{C}$ which is the second step that can get affected.
NEET-2021
Biotechnology : Principles and Processes
171117
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
1 Polysaccharides
2 RNA
3 DNA
4 Histones
Explanation:
Various enzymes like protease RNAse, etc are added to breakdown substances like proteins, RNA, etc. - All these substances are broken down, DNA is left which is precipitated out by adding chilled ethanol. - Histones are special proteins that help condense DNA in a cell.
NEET-2021
Biotechnology : Principles and Processes
171118
Which statement is correct for bacterial transduction : -
1 Transfer of some genes from one bacteria to another bacteria through virus
2 Transfer of some genes from one bacteria to another bacteria by conjugation
3 Bacteria obtained its DNA directly
4 Bacteria obtained DNA from other external source
Explanation:
In transduction, genetic material from one bacteria to another by virus (bacteriophage). It was discovered by Joshua Lederberg and Norton Zinder while working on the recombination of genes in bacteria and this technique of transfer virul DNA and applied to the bacterium Salmonella typhimurium. - Transduction is used for gene mapping \& analysis in bacteria and also for strain construction.
171113
Which of the following is not a component of downstream processing?
1 Separation
2 Purification
3 Preservation
4 Expression
Explanation:
Expression is not a component of downstream processing. Downstream processing involves the separation and purification of products synthesized in bioreactors. After completion of the process, the products formed are preserved for future use.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171114
Stirred-tank bioreactors have been designed for
1 purification of product
2 addition of preservatives to the product
3 availability of oxygen throughout the process
4 ensuring anaerobic conditions in the culture vessel
Explanation:
In the stirred - tank bioreactor, the stirrer facilitates even mixing and oxygen availability throughout the bioreactor. - Stirred tank bioreactors have several advantages over shake flasks - (i) Small volumes of culture can be taken out from the rector for sampling or testing. (ii) It has a foam breaker for regulating the foam. (iii) It has a control system that regulates the temperature and $\mathrm{PH}$.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171116
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 Ligation
2 Annealin
3 Extension
4 Denaturation
Explanation:
High temperature about $94^{\circ} \mathrm{C}$ is required for the process of denaturation which is the first step of PCR. - Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase. - Annealing is performed at $50^{\circ}-60^{\circ} \mathrm{C}$ which is the second step that can get affected.
NEET-2021
Biotechnology : Principles and Processes
171117
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
1 Polysaccharides
2 RNA
3 DNA
4 Histones
Explanation:
Various enzymes like protease RNAse, etc are added to breakdown substances like proteins, RNA, etc. - All these substances are broken down, DNA is left which is precipitated out by adding chilled ethanol. - Histones are special proteins that help condense DNA in a cell.
NEET-2021
Biotechnology : Principles and Processes
171118
Which statement is correct for bacterial transduction : -
1 Transfer of some genes from one bacteria to another bacteria through virus
2 Transfer of some genes from one bacteria to another bacteria by conjugation
3 Bacteria obtained its DNA directly
4 Bacteria obtained DNA from other external source
Explanation:
In transduction, genetic material from one bacteria to another by virus (bacteriophage). It was discovered by Joshua Lederberg and Norton Zinder while working on the recombination of genes in bacteria and this technique of transfer virul DNA and applied to the bacterium Salmonella typhimurium. - Transduction is used for gene mapping \& analysis in bacteria and also for strain construction.
171113
Which of the following is not a component of downstream processing?
1 Separation
2 Purification
3 Preservation
4 Expression
Explanation:
Expression is not a component of downstream processing. Downstream processing involves the separation and purification of products synthesized in bioreactors. After completion of the process, the products formed are preserved for future use.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171114
Stirred-tank bioreactors have been designed for
1 purification of product
2 addition of preservatives to the product
3 availability of oxygen throughout the process
4 ensuring anaerobic conditions in the culture vessel
Explanation:
In the stirred - tank bioreactor, the stirrer facilitates even mixing and oxygen availability throughout the bioreactor. - Stirred tank bioreactors have several advantages over shake flasks - (i) Small volumes of culture can be taken out from the rector for sampling or testing. (ii) It has a foam breaker for regulating the foam. (iii) It has a control system that regulates the temperature and $\mathrm{PH}$.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171116
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 Ligation
2 Annealin
3 Extension
4 Denaturation
Explanation:
High temperature about $94^{\circ} \mathrm{C}$ is required for the process of denaturation which is the first step of PCR. - Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase. - Annealing is performed at $50^{\circ}-60^{\circ} \mathrm{C}$ which is the second step that can get affected.
NEET-2021
Biotechnology : Principles and Processes
171117
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
1 Polysaccharides
2 RNA
3 DNA
4 Histones
Explanation:
Various enzymes like protease RNAse, etc are added to breakdown substances like proteins, RNA, etc. - All these substances are broken down, DNA is left which is precipitated out by adding chilled ethanol. - Histones are special proteins that help condense DNA in a cell.
NEET-2021
Biotechnology : Principles and Processes
171118
Which statement is correct for bacterial transduction : -
1 Transfer of some genes from one bacteria to another bacteria through virus
2 Transfer of some genes from one bacteria to another bacteria by conjugation
3 Bacteria obtained its DNA directly
4 Bacteria obtained DNA from other external source
Explanation:
In transduction, genetic material from one bacteria to another by virus (bacteriophage). It was discovered by Joshua Lederberg and Norton Zinder while working on the recombination of genes in bacteria and this technique of transfer virul DNA and applied to the bacterium Salmonella typhimurium. - Transduction is used for gene mapping \& analysis in bacteria and also for strain construction.
171113
Which of the following is not a component of downstream processing?
1 Separation
2 Purification
3 Preservation
4 Expression
Explanation:
Expression is not a component of downstream processing. Downstream processing involves the separation and purification of products synthesized in bioreactors. After completion of the process, the products formed are preserved for future use.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171114
Stirred-tank bioreactors have been designed for
1 purification of product
2 addition of preservatives to the product
3 availability of oxygen throughout the process
4 ensuring anaerobic conditions in the culture vessel
Explanation:
In the stirred - tank bioreactor, the stirrer facilitates even mixing and oxygen availability throughout the bioreactor. - Stirred tank bioreactors have several advantages over shake flasks - (i) Small volumes of culture can be taken out from the rector for sampling or testing. (ii) It has a foam breaker for regulating the foam. (iii) It has a control system that regulates the temperature and $\mathrm{PH}$.
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171116
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 Ligation
2 Annealin
3 Extension
4 Denaturation
Explanation:
High temperature about $94^{\circ} \mathrm{C}$ is required for the process of denaturation which is the first step of PCR. - Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase. - Annealing is performed at $50^{\circ}-60^{\circ} \mathrm{C}$ which is the second step that can get affected.
NEET-2021
Biotechnology : Principles and Processes
171117
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
1 Polysaccharides
2 RNA
3 DNA
4 Histones
Explanation:
Various enzymes like protease RNAse, etc are added to breakdown substances like proteins, RNA, etc. - All these substances are broken down, DNA is left which is precipitated out by adding chilled ethanol. - Histones are special proteins that help condense DNA in a cell.
NEET-2021
Biotechnology : Principles and Processes
171118
Which statement is correct for bacterial transduction : -
1 Transfer of some genes from one bacteria to another bacteria through virus
2 Transfer of some genes from one bacteria to another bacteria by conjugation
3 Bacteria obtained its DNA directly
4 Bacteria obtained DNA from other external source
Explanation:
In transduction, genetic material from one bacteria to another by virus (bacteriophage). It was discovered by Joshua Lederberg and Norton Zinder while working on the recombination of genes in bacteria and this technique of transfer virul DNA and applied to the bacterium Salmonella typhimurium. - Transduction is used for gene mapping \& analysis in bacteria and also for strain construction.
