03. Method of Recombinant DNA Technology
Biotechnology : Principles and Processes

171119 Which of the following is not a desirable feature of a cloning vector?

1 Presence of two or more recognition site
2 Presence of origin of replication
3 Presence of a marker gene
4 Presence of single restriction enzyme site
Biotechnology : Principles and Processes

171128 From the given combinations of steps in PCR, identify the enzyme dependent step/s.

1 Annealing and extension
2 Annealing and denaturation
3 Denaturation and extension
4 Extension only.
Biotechnology : Principles and Processes

171131 Genetically transformed host cells can be selected by
(i) Antibiotic resistant genes
(ii) Radioactive ds DNA having complementarity to the whole length of desired DNA
(iii) Coding sequence of $\beta$-galactosidase
(iv) Radioactive ss DNA having complementarity to atleast one part of desired DNA
(v) Radioactive ds RNA having complementarity to the whole length of desire DNA Options:

1 i, ii, v
2 ii, iii, v
3 i, ii, iii, v
4 i, iii, iv
Biotechnology : Principles and Processes

171133 Polyethylene glycol method is used for :

1 Energy production from sewage
2 Gene transfer without a vector
3 Biodiesel production
4 Seedless fruit production
Biotechnology : Principles and Processes

171119 Which of the following is not a desirable feature of a cloning vector?

1 Presence of two or more recognition site
2 Presence of origin of replication
3 Presence of a marker gene
4 Presence of single restriction enzyme site
Biotechnology : Principles and Processes

171128 From the given combinations of steps in PCR, identify the enzyme dependent step/s.

1 Annealing and extension
2 Annealing and denaturation
3 Denaturation and extension
4 Extension only.
Biotechnology : Principles and Processes

171131 Genetically transformed host cells can be selected by
(i) Antibiotic resistant genes
(ii) Radioactive ds DNA having complementarity to the whole length of desired DNA
(iii) Coding sequence of $\beta$-galactosidase
(iv) Radioactive ss DNA having complementarity to atleast one part of desired DNA
(v) Radioactive ds RNA having complementarity to the whole length of desire DNA Options:

1 i, ii, v
2 ii, iii, v
3 i, ii, iii, v
4 i, iii, iv
Biotechnology : Principles and Processes

171133 Polyethylene glycol method is used for :

1 Energy production from sewage
2 Gene transfer without a vector
3 Biodiesel production
4 Seedless fruit production
Biotechnology : Principles and Processes

171119 Which of the following is not a desirable feature of a cloning vector?

1 Presence of two or more recognition site
2 Presence of origin of replication
3 Presence of a marker gene
4 Presence of single restriction enzyme site
Biotechnology : Principles and Processes

171128 From the given combinations of steps in PCR, identify the enzyme dependent step/s.

1 Annealing and extension
2 Annealing and denaturation
3 Denaturation and extension
4 Extension only.
Biotechnology : Principles and Processes

171131 Genetically transformed host cells can be selected by
(i) Antibiotic resistant genes
(ii) Radioactive ds DNA having complementarity to the whole length of desired DNA
(iii) Coding sequence of $\beta$-galactosidase
(iv) Radioactive ss DNA having complementarity to atleast one part of desired DNA
(v) Radioactive ds RNA having complementarity to the whole length of desire DNA Options:

1 i, ii, v
2 ii, iii, v
3 i, ii, iii, v
4 i, iii, iv
Biotechnology : Principles and Processes

171133 Polyethylene glycol method is used for :

1 Energy production from sewage
2 Gene transfer without a vector
3 Biodiesel production
4 Seedless fruit production
NEET Test Series from KOTA - 10 Papers In MS WORD WhatsApp Here
Biotechnology : Principles and Processes

171119 Which of the following is not a desirable feature of a cloning vector?

1 Presence of two or more recognition site
2 Presence of origin of replication
3 Presence of a marker gene
4 Presence of single restriction enzyme site
Biotechnology : Principles and Processes

171128 From the given combinations of steps in PCR, identify the enzyme dependent step/s.

1 Annealing and extension
2 Annealing and denaturation
3 Denaturation and extension
4 Extension only.
Biotechnology : Principles and Processes

171131 Genetically transformed host cells can be selected by
(i) Antibiotic resistant genes
(ii) Radioactive ds DNA having complementarity to the whole length of desired DNA
(iii) Coding sequence of $\beta$-galactosidase
(iv) Radioactive ss DNA having complementarity to atleast one part of desired DNA
(v) Radioactive ds RNA having complementarity to the whole length of desire DNA Options:

1 i, ii, v
2 ii, iii, v
3 i, ii, iii, v
4 i, iii, iv
Biotechnology : Principles and Processes

171133 Polyethylene glycol method is used for :

1 Energy production from sewage
2 Gene transfer without a vector
3 Biodiesel production
4 Seedless fruit production