171063
The specific palindromic sequence which is recognized by EcoRl is
1 5' - GGAACC - 3' $3^{\prime}$ - CCTTGG - 5
2 5' - CTTAAG - 3' $3^{\prime}$ - GAATTC - 5
3 5' - GGATCC - 3' 3' - CCTAGG - 5
4 5' $^{\prime}$ - GAATTC - 3' 3 ' - CTTAAG - 5
Explanation:
The specific palindromic sequence which is recognized by EcoRI is. 5' - GAATTC - 3' $3^{\prime}$ - CTTAAG - 5'
NEET-2020 Phase-I
Biotechnology : Principles and Processes
171064
Select the correct statement from the following
1 PCR is used for isolation and separation of gene of interest
2 Gel electrophoresis is used for amplification of a DNA segment
3 The polymerase enzyme joins the gene of interest and the vector DNA
4 Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions
Explanation:
Restriction enzyme digestions are performed by incubating purified DNA molecule with the restriction enzyme of optimum conditions is the correct answer. - Gel electrophoresis is used for the isolation and separation of DNA fragments. - The polymerase enzymes use DNA templates to catalyse polymerization of deoxynucleotides. - Polymerase chain reaction is used for the amplification of DNA segment.
NEET-2020 Phase-II
Biotechnology : Principles and Processes
171068
Commonly used vectors for human genome sequencing are:-
1 T- DNA
2 BAC and YAC
3 Expression vectors
4 T/A cloning vectors
Explanation:
Bacterial artificial chromosome (BAC) vectors are based on natural, extra-chromosomal plasmid of E. coli. BAC vector contains genes for replication and maintenance of the F-factor, a selectable marker and cloning site. These vectors can accommodate upto $300-350 \mathrm{~kb}$ of foreign DNA and are also being used in genome sequencing project. - Yeast Artificial Chromosome (YAC) vectors are used to clone DNA fragments of more than $1 \mathrm{Mb}$ in size. - Therefore, they have been exploited extensively in mapping the large genomes, e.g., in the Human Genome Project. These vectors contain the telomeric sequence, the centromere and the autonomously replicating sequence from yeast chromosomes.
AIPMT-2014
Biotechnology : Principles and Processes
171069
Which vector can clone only a small fragment of DNA?
1 Bacterial artificial chromosome
2 Yeast artificial chromosome
3 Plasmid
4 Cosmid
Explanation:
Plasmids can clone only a small fragment of DNA. Plasmids are extra chromosomal, autonomously replicating circular, double-stranded DNA molecules. They are small, circular, extra-chromosomal DNA which is present in bacteria and can replicate autonomously. They naturally occur in some yeasts and several bacteria. Plasmids provide bacteria with genetic advantages, such as antibiotic resistance. These plasmids are altered to be used as vectors as they can clone DNA fragments close to size $10 \mathrm{kbp}$. Plasmid pBR322 is one of the earliest and most common cloning vectors.
171063
The specific palindromic sequence which is recognized by EcoRl is
1 5' - GGAACC - 3' $3^{\prime}$ - CCTTGG - 5
2 5' - CTTAAG - 3' $3^{\prime}$ - GAATTC - 5
3 5' - GGATCC - 3' 3' - CCTAGG - 5
4 5' $^{\prime}$ - GAATTC - 3' 3 ' - CTTAAG - 5
Explanation:
The specific palindromic sequence which is recognized by EcoRI is. 5' - GAATTC - 3' $3^{\prime}$ - CTTAAG - 5'
NEET-2020 Phase-I
Biotechnology : Principles and Processes
171064
Select the correct statement from the following
1 PCR is used for isolation and separation of gene of interest
2 Gel electrophoresis is used for amplification of a DNA segment
3 The polymerase enzyme joins the gene of interest and the vector DNA
4 Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions
Explanation:
Restriction enzyme digestions are performed by incubating purified DNA molecule with the restriction enzyme of optimum conditions is the correct answer. - Gel electrophoresis is used for the isolation and separation of DNA fragments. - The polymerase enzymes use DNA templates to catalyse polymerization of deoxynucleotides. - Polymerase chain reaction is used for the amplification of DNA segment.
NEET-2020 Phase-II
Biotechnology : Principles and Processes
171068
Commonly used vectors for human genome sequencing are:-
1 T- DNA
2 BAC and YAC
3 Expression vectors
4 T/A cloning vectors
Explanation:
Bacterial artificial chromosome (BAC) vectors are based on natural, extra-chromosomal plasmid of E. coli. BAC vector contains genes for replication and maintenance of the F-factor, a selectable marker and cloning site. These vectors can accommodate upto $300-350 \mathrm{~kb}$ of foreign DNA and are also being used in genome sequencing project. - Yeast Artificial Chromosome (YAC) vectors are used to clone DNA fragments of more than $1 \mathrm{Mb}$ in size. - Therefore, they have been exploited extensively in mapping the large genomes, e.g., in the Human Genome Project. These vectors contain the telomeric sequence, the centromere and the autonomously replicating sequence from yeast chromosomes.
AIPMT-2014
Biotechnology : Principles and Processes
171069
Which vector can clone only a small fragment of DNA?
1 Bacterial artificial chromosome
2 Yeast artificial chromosome
3 Plasmid
4 Cosmid
Explanation:
Plasmids can clone only a small fragment of DNA. Plasmids are extra chromosomal, autonomously replicating circular, double-stranded DNA molecules. They are small, circular, extra-chromosomal DNA which is present in bacteria and can replicate autonomously. They naturally occur in some yeasts and several bacteria. Plasmids provide bacteria with genetic advantages, such as antibiotic resistance. These plasmids are altered to be used as vectors as they can clone DNA fragments close to size $10 \mathrm{kbp}$. Plasmid pBR322 is one of the earliest and most common cloning vectors.
171063
The specific palindromic sequence which is recognized by EcoRl is
1 5' - GGAACC - 3' $3^{\prime}$ - CCTTGG - 5
2 5' - CTTAAG - 3' $3^{\prime}$ - GAATTC - 5
3 5' - GGATCC - 3' 3' - CCTAGG - 5
4 5' $^{\prime}$ - GAATTC - 3' 3 ' - CTTAAG - 5
Explanation:
The specific palindromic sequence which is recognized by EcoRI is. 5' - GAATTC - 3' $3^{\prime}$ - CTTAAG - 5'
NEET-2020 Phase-I
Biotechnology : Principles and Processes
171064
Select the correct statement from the following
1 PCR is used for isolation and separation of gene of interest
2 Gel electrophoresis is used for amplification of a DNA segment
3 The polymerase enzyme joins the gene of interest and the vector DNA
4 Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions
Explanation:
Restriction enzyme digestions are performed by incubating purified DNA molecule with the restriction enzyme of optimum conditions is the correct answer. - Gel electrophoresis is used for the isolation and separation of DNA fragments. - The polymerase enzymes use DNA templates to catalyse polymerization of deoxynucleotides. - Polymerase chain reaction is used for the amplification of DNA segment.
NEET-2020 Phase-II
Biotechnology : Principles and Processes
171068
Commonly used vectors for human genome sequencing are:-
1 T- DNA
2 BAC and YAC
3 Expression vectors
4 T/A cloning vectors
Explanation:
Bacterial artificial chromosome (BAC) vectors are based on natural, extra-chromosomal plasmid of E. coli. BAC vector contains genes for replication and maintenance of the F-factor, a selectable marker and cloning site. These vectors can accommodate upto $300-350 \mathrm{~kb}$ of foreign DNA and are also being used in genome sequencing project. - Yeast Artificial Chromosome (YAC) vectors are used to clone DNA fragments of more than $1 \mathrm{Mb}$ in size. - Therefore, they have been exploited extensively in mapping the large genomes, e.g., in the Human Genome Project. These vectors contain the telomeric sequence, the centromere and the autonomously replicating sequence from yeast chromosomes.
AIPMT-2014
Biotechnology : Principles and Processes
171069
Which vector can clone only a small fragment of DNA?
1 Bacterial artificial chromosome
2 Yeast artificial chromosome
3 Plasmid
4 Cosmid
Explanation:
Plasmids can clone only a small fragment of DNA. Plasmids are extra chromosomal, autonomously replicating circular, double-stranded DNA molecules. They are small, circular, extra-chromosomal DNA which is present in bacteria and can replicate autonomously. They naturally occur in some yeasts and several bacteria. Plasmids provide bacteria with genetic advantages, such as antibiotic resistance. These plasmids are altered to be used as vectors as they can clone DNA fragments close to size $10 \mathrm{kbp}$. Plasmid pBR322 is one of the earliest and most common cloning vectors.
171063
The specific palindromic sequence which is recognized by EcoRl is
1 5' - GGAACC - 3' $3^{\prime}$ - CCTTGG - 5
2 5' - CTTAAG - 3' $3^{\prime}$ - GAATTC - 5
3 5' - GGATCC - 3' 3' - CCTAGG - 5
4 5' $^{\prime}$ - GAATTC - 3' 3 ' - CTTAAG - 5
Explanation:
The specific palindromic sequence which is recognized by EcoRI is. 5' - GAATTC - 3' $3^{\prime}$ - CTTAAG - 5'
NEET-2020 Phase-I
Biotechnology : Principles and Processes
171064
Select the correct statement from the following
1 PCR is used for isolation and separation of gene of interest
2 Gel electrophoresis is used for amplification of a DNA segment
3 The polymerase enzyme joins the gene of interest and the vector DNA
4 Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions
Explanation:
Restriction enzyme digestions are performed by incubating purified DNA molecule with the restriction enzyme of optimum conditions is the correct answer. - Gel electrophoresis is used for the isolation and separation of DNA fragments. - The polymerase enzymes use DNA templates to catalyse polymerization of deoxynucleotides. - Polymerase chain reaction is used for the amplification of DNA segment.
NEET-2020 Phase-II
Biotechnology : Principles and Processes
171068
Commonly used vectors for human genome sequencing are:-
1 T- DNA
2 BAC and YAC
3 Expression vectors
4 T/A cloning vectors
Explanation:
Bacterial artificial chromosome (BAC) vectors are based on natural, extra-chromosomal plasmid of E. coli. BAC vector contains genes for replication and maintenance of the F-factor, a selectable marker and cloning site. These vectors can accommodate upto $300-350 \mathrm{~kb}$ of foreign DNA and are also being used in genome sequencing project. - Yeast Artificial Chromosome (YAC) vectors are used to clone DNA fragments of more than $1 \mathrm{Mb}$ in size. - Therefore, they have been exploited extensively in mapping the large genomes, e.g., in the Human Genome Project. These vectors contain the telomeric sequence, the centromere and the autonomously replicating sequence from yeast chromosomes.
AIPMT-2014
Biotechnology : Principles and Processes
171069
Which vector can clone only a small fragment of DNA?
1 Bacterial artificial chromosome
2 Yeast artificial chromosome
3 Plasmid
4 Cosmid
Explanation:
Plasmids can clone only a small fragment of DNA. Plasmids are extra chromosomal, autonomously replicating circular, double-stranded DNA molecules. They are small, circular, extra-chromosomal DNA which is present in bacteria and can replicate autonomously. They naturally occur in some yeasts and several bacteria. Plasmids provide bacteria with genetic advantages, such as antibiotic resistance. These plasmids are altered to be used as vectors as they can clone DNA fragments close to size $10 \mathrm{kbp}$. Plasmid pBR322 is one of the earliest and most common cloning vectors.
