171070
The two antibiotic resistance genes on vector ${ }_p B R 322$ are for
1 Tetracycline and Kanamycin
2 Ampicillin and Tetracycline
3 Ampicillin and Chloramphenicol
4 Chloramphenicol and Tetracycline
Explanation:
${ }_{\mathrm{p}} \mathrm{BR} 322$ was one of the first widely used E.coli cloning vectors in which 'p' stands for plasmid and BR stands for Bolivar and Rodriguez, the scientists who constructed it. The plasmid ${ }_{\mathrm{p}}$ BR322 vector carries the genes for tetracycline $\left(\right.$ tet $\left.^R\right)$ and ampicillin $\left(a m p^R\right)$ resistance. There genes are useful to identify and select the transformants and non - transformants.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171072
An enzyme catalysing the removal of nucleotides from ends of DNA is :
1 Protease
2 DNA ligase
3 Endonuclease
4 Exonuclease
Explanation:
An enzyme catalyzing the removal of nucleotides from ends of DNA is exonuclease. Exonucleases remove nucleotides from the ends of the DNA. Endonucleases make cuts at specific positions within the DNA. - DNA ligase is sealing enzyme (also called as genetic gum), which is responsible for joining of two individual fragments of DNA. - Protease (Proteolytic enzymes) are enzymes that break down protein.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171078
A tumor inducing plasmid widely used in the production of transgenic plants is that of
1 Escherichia coli
2 Bacillus thuringiensis
3 Staphylococcus aureus
4 Agrobacterium tumefaciens
Explanation:
Agrobacterium tumefaciens is a plant pathogen with the capacity to deliver a segment of oncogenic DNA carried on a large plasmid called the tumor-inducing or Ti plasmid to susceptible plant cells. - The transferred T-DNA is then integrated into the host genome and transcribed with it. This ability of Agrobacterium tumefaciens to transfer the T-DNA in host genome is explored in genetic engineering to transfer the desired DNA segment, carrying the gene of the interest, into genome of selected organisms.
AIIMS-2005
Biotechnology : Principles and Processes
171080
Chimeric DNA is
1 Gene clone
2 Recombinant-DNA
3 Transposon
4 Vector shuttle
Explanation:
Recombinant DNA molecules are sometimes called chimeric DNA, because they can be made of material from two different species, like the mythical chimera. - A chimera is essentially a single organism that is made up of cells from two or more individuals i.e., it contains two set of DNA, with the code to make two separate organisms. - R-DNA technology uses palindromic sequences and leads to the production of sticky and blunt ends.
AIIMS-25.05.2019 Shift-II
Biotechnology : Principles and Processes
171081
What does ' $R$ ' indicate in EcoRI?
1 Enzyme isolated from species of bacteria
2 Genus of bacteria
3 Sequence of enzyme
4 Species of bacteria
Explanation:
EcoRI is isolated from the bacterium Escherichia Coli (E.Coli). - Eco represents the species of bacteria. - $\mathrm{R}$ represents the strain of the bacteria which is $\mathrm{RY}-13$ in this case. - EcoRI is pronounced as Eco-R-one which is a restriction endonuclease enzyme.
171070
The two antibiotic resistance genes on vector ${ }_p B R 322$ are for
1 Tetracycline and Kanamycin
2 Ampicillin and Tetracycline
3 Ampicillin and Chloramphenicol
4 Chloramphenicol and Tetracycline
Explanation:
${ }_{\mathrm{p}} \mathrm{BR} 322$ was one of the first widely used E.coli cloning vectors in which 'p' stands for plasmid and BR stands for Bolivar and Rodriguez, the scientists who constructed it. The plasmid ${ }_{\mathrm{p}}$ BR322 vector carries the genes for tetracycline $\left(\right.$ tet $\left.^R\right)$ and ampicillin $\left(a m p^R\right)$ resistance. There genes are useful to identify and select the transformants and non - transformants.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171072
An enzyme catalysing the removal of nucleotides from ends of DNA is :
1 Protease
2 DNA ligase
3 Endonuclease
4 Exonuclease
Explanation:
An enzyme catalyzing the removal of nucleotides from ends of DNA is exonuclease. Exonucleases remove nucleotides from the ends of the DNA. Endonucleases make cuts at specific positions within the DNA. - DNA ligase is sealing enzyme (also called as genetic gum), which is responsible for joining of two individual fragments of DNA. - Protease (Proteolytic enzymes) are enzymes that break down protein.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171078
A tumor inducing plasmid widely used in the production of transgenic plants is that of
1 Escherichia coli
2 Bacillus thuringiensis
3 Staphylococcus aureus
4 Agrobacterium tumefaciens
Explanation:
Agrobacterium tumefaciens is a plant pathogen with the capacity to deliver a segment of oncogenic DNA carried on a large plasmid called the tumor-inducing or Ti plasmid to susceptible plant cells. - The transferred T-DNA is then integrated into the host genome and transcribed with it. This ability of Agrobacterium tumefaciens to transfer the T-DNA in host genome is explored in genetic engineering to transfer the desired DNA segment, carrying the gene of the interest, into genome of selected organisms.
AIIMS-2005
Biotechnology : Principles and Processes
171080
Chimeric DNA is
1 Gene clone
2 Recombinant-DNA
3 Transposon
4 Vector shuttle
Explanation:
Recombinant DNA molecules are sometimes called chimeric DNA, because they can be made of material from two different species, like the mythical chimera. - A chimera is essentially a single organism that is made up of cells from two or more individuals i.e., it contains two set of DNA, with the code to make two separate organisms. - R-DNA technology uses palindromic sequences and leads to the production of sticky and blunt ends.
AIIMS-25.05.2019 Shift-II
Biotechnology : Principles and Processes
171081
What does ' $R$ ' indicate in EcoRI?
1 Enzyme isolated from species of bacteria
2 Genus of bacteria
3 Sequence of enzyme
4 Species of bacteria
Explanation:
EcoRI is isolated from the bacterium Escherichia Coli (E.Coli). - Eco represents the species of bacteria. - $\mathrm{R}$ represents the strain of the bacteria which is $\mathrm{RY}-13$ in this case. - EcoRI is pronounced as Eco-R-one which is a restriction endonuclease enzyme.
171070
The two antibiotic resistance genes on vector ${ }_p B R 322$ are for
1 Tetracycline and Kanamycin
2 Ampicillin and Tetracycline
3 Ampicillin and Chloramphenicol
4 Chloramphenicol and Tetracycline
Explanation:
${ }_{\mathrm{p}} \mathrm{BR} 322$ was one of the first widely used E.coli cloning vectors in which 'p' stands for plasmid and BR stands for Bolivar and Rodriguez, the scientists who constructed it. The plasmid ${ }_{\mathrm{p}}$ BR322 vector carries the genes for tetracycline $\left(\right.$ tet $\left.^R\right)$ and ampicillin $\left(a m p^R\right)$ resistance. There genes are useful to identify and select the transformants and non - transformants.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171072
An enzyme catalysing the removal of nucleotides from ends of DNA is :
1 Protease
2 DNA ligase
3 Endonuclease
4 Exonuclease
Explanation:
An enzyme catalyzing the removal of nucleotides from ends of DNA is exonuclease. Exonucleases remove nucleotides from the ends of the DNA. Endonucleases make cuts at specific positions within the DNA. - DNA ligase is sealing enzyme (also called as genetic gum), which is responsible for joining of two individual fragments of DNA. - Protease (Proteolytic enzymes) are enzymes that break down protein.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171078
A tumor inducing plasmid widely used in the production of transgenic plants is that of
1 Escherichia coli
2 Bacillus thuringiensis
3 Staphylococcus aureus
4 Agrobacterium tumefaciens
Explanation:
Agrobacterium tumefaciens is a plant pathogen with the capacity to deliver a segment of oncogenic DNA carried on a large plasmid called the tumor-inducing or Ti plasmid to susceptible plant cells. - The transferred T-DNA is then integrated into the host genome and transcribed with it. This ability of Agrobacterium tumefaciens to transfer the T-DNA in host genome is explored in genetic engineering to transfer the desired DNA segment, carrying the gene of the interest, into genome of selected organisms.
AIIMS-2005
Biotechnology : Principles and Processes
171080
Chimeric DNA is
1 Gene clone
2 Recombinant-DNA
3 Transposon
4 Vector shuttle
Explanation:
Recombinant DNA molecules are sometimes called chimeric DNA, because they can be made of material from two different species, like the mythical chimera. - A chimera is essentially a single organism that is made up of cells from two or more individuals i.e., it contains two set of DNA, with the code to make two separate organisms. - R-DNA technology uses palindromic sequences and leads to the production of sticky and blunt ends.
AIIMS-25.05.2019 Shift-II
Biotechnology : Principles and Processes
171081
What does ' $R$ ' indicate in EcoRI?
1 Enzyme isolated from species of bacteria
2 Genus of bacteria
3 Sequence of enzyme
4 Species of bacteria
Explanation:
EcoRI is isolated from the bacterium Escherichia Coli (E.Coli). - Eco represents the species of bacteria. - $\mathrm{R}$ represents the strain of the bacteria which is $\mathrm{RY}-13$ in this case. - EcoRI is pronounced as Eco-R-one which is a restriction endonuclease enzyme.
NEET Test Series from KOTA - 10 Papers In MS WORD
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Biotechnology : Principles and Processes
171070
The two antibiotic resistance genes on vector ${ }_p B R 322$ are for
1 Tetracycline and Kanamycin
2 Ampicillin and Tetracycline
3 Ampicillin and Chloramphenicol
4 Chloramphenicol and Tetracycline
Explanation:
${ }_{\mathrm{p}} \mathrm{BR} 322$ was one of the first widely used E.coli cloning vectors in which 'p' stands for plasmid and BR stands for Bolivar and Rodriguez, the scientists who constructed it. The plasmid ${ }_{\mathrm{p}}$ BR322 vector carries the genes for tetracycline $\left(\right.$ tet $\left.^R\right)$ and ampicillin $\left(a m p^R\right)$ resistance. There genes are useful to identify and select the transformants and non - transformants.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171072
An enzyme catalysing the removal of nucleotides from ends of DNA is :
1 Protease
2 DNA ligase
3 Endonuclease
4 Exonuclease
Explanation:
An enzyme catalyzing the removal of nucleotides from ends of DNA is exonuclease. Exonucleases remove nucleotides from the ends of the DNA. Endonucleases make cuts at specific positions within the DNA. - DNA ligase is sealing enzyme (also called as genetic gum), which is responsible for joining of two individual fragments of DNA. - Protease (Proteolytic enzymes) are enzymes that break down protein.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171078
A tumor inducing plasmid widely used in the production of transgenic plants is that of
1 Escherichia coli
2 Bacillus thuringiensis
3 Staphylococcus aureus
4 Agrobacterium tumefaciens
Explanation:
Agrobacterium tumefaciens is a plant pathogen with the capacity to deliver a segment of oncogenic DNA carried on a large plasmid called the tumor-inducing or Ti plasmid to susceptible plant cells. - The transferred T-DNA is then integrated into the host genome and transcribed with it. This ability of Agrobacterium tumefaciens to transfer the T-DNA in host genome is explored in genetic engineering to transfer the desired DNA segment, carrying the gene of the interest, into genome of selected organisms.
AIIMS-2005
Biotechnology : Principles and Processes
171080
Chimeric DNA is
1 Gene clone
2 Recombinant-DNA
3 Transposon
4 Vector shuttle
Explanation:
Recombinant DNA molecules are sometimes called chimeric DNA, because they can be made of material from two different species, like the mythical chimera. - A chimera is essentially a single organism that is made up of cells from two or more individuals i.e., it contains two set of DNA, with the code to make two separate organisms. - R-DNA technology uses palindromic sequences and leads to the production of sticky and blunt ends.
AIIMS-25.05.2019 Shift-II
Biotechnology : Principles and Processes
171081
What does ' $R$ ' indicate in EcoRI?
1 Enzyme isolated from species of bacteria
2 Genus of bacteria
3 Sequence of enzyme
4 Species of bacteria
Explanation:
EcoRI is isolated from the bacterium Escherichia Coli (E.Coli). - Eco represents the species of bacteria. - $\mathrm{R}$ represents the strain of the bacteria which is $\mathrm{RY}-13$ in this case. - EcoRI is pronounced as Eco-R-one which is a restriction endonuclease enzyme.
171070
The two antibiotic resistance genes on vector ${ }_p B R 322$ are for
1 Tetracycline and Kanamycin
2 Ampicillin and Tetracycline
3 Ampicillin and Chloramphenicol
4 Chloramphenicol and Tetracycline
Explanation:
${ }_{\mathrm{p}} \mathrm{BR} 322$ was one of the first widely used E.coli cloning vectors in which 'p' stands for plasmid and BR stands for Bolivar and Rodriguez, the scientists who constructed it. The plasmid ${ }_{\mathrm{p}}$ BR322 vector carries the genes for tetracycline $\left(\right.$ tet $\left.^R\right)$ and ampicillin $\left(a m p^R\right)$ resistance. There genes are useful to identify and select the transformants and non - transformants.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171072
An enzyme catalysing the removal of nucleotides from ends of DNA is :
1 Protease
2 DNA ligase
3 Endonuclease
4 Exonuclease
Explanation:
An enzyme catalyzing the removal of nucleotides from ends of DNA is exonuclease. Exonucleases remove nucleotides from the ends of the DNA. Endonucleases make cuts at specific positions within the DNA. - DNA ligase is sealing enzyme (also called as genetic gum), which is responsible for joining of two individual fragments of DNA. - Protease (Proteolytic enzymes) are enzymes that break down protein.
NEET (Odisha)-2019
Biotechnology : Principles and Processes
171078
A tumor inducing plasmid widely used in the production of transgenic plants is that of
1 Escherichia coli
2 Bacillus thuringiensis
3 Staphylococcus aureus
4 Agrobacterium tumefaciens
Explanation:
Agrobacterium tumefaciens is a plant pathogen with the capacity to deliver a segment of oncogenic DNA carried on a large plasmid called the tumor-inducing or Ti plasmid to susceptible plant cells. - The transferred T-DNA is then integrated into the host genome and transcribed with it. This ability of Agrobacterium tumefaciens to transfer the T-DNA in host genome is explored in genetic engineering to transfer the desired DNA segment, carrying the gene of the interest, into genome of selected organisms.
AIIMS-2005
Biotechnology : Principles and Processes
171080
Chimeric DNA is
1 Gene clone
2 Recombinant-DNA
3 Transposon
4 Vector shuttle
Explanation:
Recombinant DNA molecules are sometimes called chimeric DNA, because they can be made of material from two different species, like the mythical chimera. - A chimera is essentially a single organism that is made up of cells from two or more individuals i.e., it contains two set of DNA, with the code to make two separate organisms. - R-DNA technology uses palindromic sequences and leads to the production of sticky and blunt ends.
AIIMS-25.05.2019 Shift-II
Biotechnology : Principles and Processes
171081
What does ' $R$ ' indicate in EcoRI?
1 Enzyme isolated from species of bacteria
2 Genus of bacteria
3 Sequence of enzyme
4 Species of bacteria
Explanation:
EcoRI is isolated from the bacterium Escherichia Coli (E.Coli). - Eco represents the species of bacteria. - $\mathrm{R}$ represents the strain of the bacteria which is $\mathrm{RY}-13$ in this case. - EcoRI is pronounced as Eco-R-one which is a restriction endonuclease enzyme.
