Tools of Biotechnology
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347628 Which of the following statement is incorrect?

1 Each restriction endonuclease recognises a specific palindromic nucleotide sequence
2 Specific base sequence is known as recognition sequence
3 Restriction enzymes are made up of nucleotides
4 Restriction enzymes belong to enzymes called nucleases
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347629 Match the following
Column I
Column II
A

Thermus aquaticus
P
Plasmid
B
pBR322
Q
DNA Polymerase
C
Ligase
R
Restriction endonuclease
D

Hind II
S
Molecular glue

1 \({\text{A - Q, B - P, C - S, D - R}}\)
2 \({\text{A - P, B - S, C - R, D - Q}}\)
3 \({\text{A - R, B - Q, C - S, D - P}}\)
4 \({\text{A - Q, B - S, C - P, D - R}}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347630 Assertion :
Restriction endonucleases are also called 'molecular scissors'.
Reason :
When fragments generated by restriction endonucleases are mixed, they join together due to their sticky ends.

1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347628 Which of the following statement is incorrect?

1 Each restriction endonuclease recognises a specific palindromic nucleotide sequence
2 Specific base sequence is known as recognition sequence
3 Restriction enzymes are made up of nucleotides
4 Restriction enzymes belong to enzymes called nucleases
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347629 Match the following
Column I
Column II
A

Thermus aquaticus
P
Plasmid
B
pBR322
Q
DNA Polymerase
C
Ligase
R
Restriction endonuclease
D

Hind II
S
Molecular glue

1 \({\text{A - Q, B - P, C - S, D - R}}\)
2 \({\text{A - P, B - S, C - R, D - Q}}\)
3 \({\text{A - R, B - Q, C - S, D - P}}\)
4 \({\text{A - Q, B - S, C - P, D - R}}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347630 Assertion :
Restriction endonucleases are also called 'molecular scissors'.
Reason :
When fragments generated by restriction endonucleases are mixed, they join together due to their sticky ends.

1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347628 Which of the following statement is incorrect?

1 Each restriction endonuclease recognises a specific palindromic nucleotide sequence
2 Specific base sequence is known as recognition sequence
3 Restriction enzymes are made up of nucleotides
4 Restriction enzymes belong to enzymes called nucleases
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347629 Match the following
Column I
Column II
A

Thermus aquaticus
P
Plasmid
B
pBR322
Q
DNA Polymerase
C
Ligase
R
Restriction endonuclease
D

Hind II
S
Molecular glue

1 \({\text{A - Q, B - P, C - S, D - R}}\)
2 \({\text{A - P, B - S, C - R, D - Q}}\)
3 \({\text{A - R, B - Q, C - S, D - P}}\)
4 \({\text{A - Q, B - S, C - P, D - R}}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347630 Assertion :
Restriction endonucleases are also called 'molecular scissors'.
Reason :
When fragments generated by restriction endonucleases are mixed, they join together due to their sticky ends.

1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect