347597
The foreign DNA and the vector are cut with the
1 Two different enzymes
2 Same restriction enzymes
3 Both 1 and 2
4 It could be same or different
Explanation:
Same restriction enzymes are used to cut both the foreign DNA and the vector, so that the resultant DNA fragments have the complementary kind of sticky ends.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347598
Select the correct answer I. Restriction enzymes cut the strand of DNA a little away from the end of the palindrome sites, but between the same two bases on the opposite strands. II. The overhanging stretches are called sticky ends on each strand
1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
Explanation:
Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347599
Specific DNA sequence GAATTC is cut by which of the following restriction endonuclease
1 Hind II
2 Hind III
3 Eco RII
4 Eco RI
Explanation:
EcoRI is the restriction enzyme, derived from Escherichia coli, strain R and is the first to be isolated. EcoRI recognizes and cuts at the specific restriction site 5'-GAATTC-3' and produces sticky ends. This is the common enzyme that is used as a tool for the restriction of the bacterial plasmid and desired genes in molecular biology techniques.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347600
How many fragments will be generated, if a closed circular DNA molecule is digested using a restriction enzyme having six recognition sites on the DNA?
1 12
2 6
3 7
4 5
Explanation:
When a closed circular DNA molecule is digested with a restriction enzyme having six recognition sites, it will produce 6 DNA fragments.
347597
The foreign DNA and the vector are cut with the
1 Two different enzymes
2 Same restriction enzymes
3 Both 1 and 2
4 It could be same or different
Explanation:
Same restriction enzymes are used to cut both the foreign DNA and the vector, so that the resultant DNA fragments have the complementary kind of sticky ends.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347598
Select the correct answer I. Restriction enzymes cut the strand of DNA a little away from the end of the palindrome sites, but between the same two bases on the opposite strands. II. The overhanging stretches are called sticky ends on each strand
1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
Explanation:
Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347599
Specific DNA sequence GAATTC is cut by which of the following restriction endonuclease
1 Hind II
2 Hind III
3 Eco RII
4 Eco RI
Explanation:
EcoRI is the restriction enzyme, derived from Escherichia coli, strain R and is the first to be isolated. EcoRI recognizes and cuts at the specific restriction site 5'-GAATTC-3' and produces sticky ends. This is the common enzyme that is used as a tool for the restriction of the bacterial plasmid and desired genes in molecular biology techniques.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347600
How many fragments will be generated, if a closed circular DNA molecule is digested using a restriction enzyme having six recognition sites on the DNA?
1 12
2 6
3 7
4 5
Explanation:
When a closed circular DNA molecule is digested with a restriction enzyme having six recognition sites, it will produce 6 DNA fragments.
347597
The foreign DNA and the vector are cut with the
1 Two different enzymes
2 Same restriction enzymes
3 Both 1 and 2
4 It could be same or different
Explanation:
Same restriction enzymes are used to cut both the foreign DNA and the vector, so that the resultant DNA fragments have the complementary kind of sticky ends.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347598
Select the correct answer I. Restriction enzymes cut the strand of DNA a little away from the end of the palindrome sites, but between the same two bases on the opposite strands. II. The overhanging stretches are called sticky ends on each strand
1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
Explanation:
Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347599
Specific DNA sequence GAATTC is cut by which of the following restriction endonuclease
1 Hind II
2 Hind III
3 Eco RII
4 Eco RI
Explanation:
EcoRI is the restriction enzyme, derived from Escherichia coli, strain R and is the first to be isolated. EcoRI recognizes and cuts at the specific restriction site 5'-GAATTC-3' and produces sticky ends. This is the common enzyme that is used as a tool for the restriction of the bacterial plasmid and desired genes in molecular biology techniques.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347600
How many fragments will be generated, if a closed circular DNA molecule is digested using a restriction enzyme having six recognition sites on the DNA?
1 12
2 6
3 7
4 5
Explanation:
When a closed circular DNA molecule is digested with a restriction enzyme having six recognition sites, it will produce 6 DNA fragments.
347597
The foreign DNA and the vector are cut with the
1 Two different enzymes
2 Same restriction enzymes
3 Both 1 and 2
4 It could be same or different
Explanation:
Same restriction enzymes are used to cut both the foreign DNA and the vector, so that the resultant DNA fragments have the complementary kind of sticky ends.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347598
Select the correct answer I. Restriction enzymes cut the strand of DNA a little away from the end of the palindrome sites, but between the same two bases on the opposite strands. II. The overhanging stretches are called sticky ends on each strand
1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
Explanation:
Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347599
Specific DNA sequence GAATTC is cut by which of the following restriction endonuclease
1 Hind II
2 Hind III
3 Eco RII
4 Eco RI
Explanation:
EcoRI is the restriction enzyme, derived from Escherichia coli, strain R and is the first to be isolated. EcoRI recognizes and cuts at the specific restriction site 5'-GAATTC-3' and produces sticky ends. This is the common enzyme that is used as a tool for the restriction of the bacterial plasmid and desired genes in molecular biology techniques.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347600
How many fragments will be generated, if a closed circular DNA molecule is digested using a restriction enzyme having six recognition sites on the DNA?
1 12
2 6
3 7
4 5
Explanation:
When a closed circular DNA molecule is digested with a restriction enzyme having six recognition sites, it will produce 6 DNA fragments.
