347580
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1 Degenerate primer sequence
2 Okazaki sequences
3 Palidromic Nucleotide sequences
4 Poly (A) tail sequences
Explanation:
Endonuclease will recognize the palindromic sequence for cutting
NEET - 2021
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347581
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
1 The enzyme cuts DNA molecule at identified position within the DNA.
2 The enzyme binds DNA at specific sites and cuts only one of the two strands.
3 The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
4 The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
Explanation:
Restriction enzymes cut DNA molecules at a particular point by recognising a specific sequence. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugarphosphate backbone.
NEET - 2019
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347582
The restriction enzyme isolated and characterised five years later to 1963 , responsible for the cleavage of following sequence consisting of six base pairs is 5'-GTCGAC 3' 3'- CAGCTG 5'
1 Pst I
2 Bam HI
3 Hind II
4 Eco RI
Explanation:
The restriction enzyme responsible for the cleavage of the given sequence is Hind II. \(\begin{gathered}\downarrow \\5^{\prime}-G T C \quad G A C-3^{\prime} \\3^{\prime}-C A G \quad C T G-5^{\prime} \\\uparrow\end{gathered}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347583
The sticky ends of a fragmented DNA molecule are made up of
1 Unpaired bases
2 Paired bases
3 Methyl group
4 Endonuclease
Explanation:
Sticky ends of DNA are also called as staggerred end, it is also called as cohesive end. Because they have unpaired bases, it can hybridize on any other complementary DNA bases.
347580
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1 Degenerate primer sequence
2 Okazaki sequences
3 Palidromic Nucleotide sequences
4 Poly (A) tail sequences
Explanation:
Endonuclease will recognize the palindromic sequence for cutting
NEET - 2021
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347581
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
1 The enzyme cuts DNA molecule at identified position within the DNA.
2 The enzyme binds DNA at specific sites and cuts only one of the two strands.
3 The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
4 The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
Explanation:
Restriction enzymes cut DNA molecules at a particular point by recognising a specific sequence. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugarphosphate backbone.
NEET - 2019
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347582
The restriction enzyme isolated and characterised five years later to 1963 , responsible for the cleavage of following sequence consisting of six base pairs is 5'-GTCGAC 3' 3'- CAGCTG 5'
1 Pst I
2 Bam HI
3 Hind II
4 Eco RI
Explanation:
The restriction enzyme responsible for the cleavage of the given sequence is Hind II. \(\begin{gathered}\downarrow \\5^{\prime}-G T C \quad G A C-3^{\prime} \\3^{\prime}-C A G \quad C T G-5^{\prime} \\\uparrow\end{gathered}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347583
The sticky ends of a fragmented DNA molecule are made up of
1 Unpaired bases
2 Paired bases
3 Methyl group
4 Endonuclease
Explanation:
Sticky ends of DNA are also called as staggerred end, it is also called as cohesive end. Because they have unpaired bases, it can hybridize on any other complementary DNA bases.
347580
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1 Degenerate primer sequence
2 Okazaki sequences
3 Palidromic Nucleotide sequences
4 Poly (A) tail sequences
Explanation:
Endonuclease will recognize the palindromic sequence for cutting
NEET - 2021
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347581
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
1 The enzyme cuts DNA molecule at identified position within the DNA.
2 The enzyme binds DNA at specific sites and cuts only one of the two strands.
3 The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
4 The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
Explanation:
Restriction enzymes cut DNA molecules at a particular point by recognising a specific sequence. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugarphosphate backbone.
NEET - 2019
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347582
The restriction enzyme isolated and characterised five years later to 1963 , responsible for the cleavage of following sequence consisting of six base pairs is 5'-GTCGAC 3' 3'- CAGCTG 5'
1 Pst I
2 Bam HI
3 Hind II
4 Eco RI
Explanation:
The restriction enzyme responsible for the cleavage of the given sequence is Hind II. \(\begin{gathered}\downarrow \\5^{\prime}-G T C \quad G A C-3^{\prime} \\3^{\prime}-C A G \quad C T G-5^{\prime} \\\uparrow\end{gathered}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347583
The sticky ends of a fragmented DNA molecule are made up of
1 Unpaired bases
2 Paired bases
3 Methyl group
4 Endonuclease
Explanation:
Sticky ends of DNA are also called as staggerred end, it is also called as cohesive end. Because they have unpaired bases, it can hybridize on any other complementary DNA bases.
347580
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1 Degenerate primer sequence
2 Okazaki sequences
3 Palidromic Nucleotide sequences
4 Poly (A) tail sequences
Explanation:
Endonuclease will recognize the palindromic sequence for cutting
NEET - 2021
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347581
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
1 The enzyme cuts DNA molecule at identified position within the DNA.
2 The enzyme binds DNA at specific sites and cuts only one of the two strands.
3 The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
4 The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
Explanation:
Restriction enzymes cut DNA molecules at a particular point by recognising a specific sequence. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugarphosphate backbone.
NEET - 2019
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347582
The restriction enzyme isolated and characterised five years later to 1963 , responsible for the cleavage of following sequence consisting of six base pairs is 5'-GTCGAC 3' 3'- CAGCTG 5'
1 Pst I
2 Bam HI
3 Hind II
4 Eco RI
Explanation:
The restriction enzyme responsible for the cleavage of the given sequence is Hind II. \(\begin{gathered}\downarrow \\5^{\prime}-G T C \quad G A C-3^{\prime} \\3^{\prime}-C A G \quad C T G-5^{\prime} \\\uparrow\end{gathered}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347583
The sticky ends of a fragmented DNA molecule are made up of
1 Unpaired bases
2 Paired bases
3 Methyl group
4 Endonuclease
Explanation:
Sticky ends of DNA are also called as staggerred end, it is also called as cohesive end. Because they have unpaired bases, it can hybridize on any other complementary DNA bases.
