347571
The enzymes which are absolutely necessary for recombinant DNA technology are:
1 Restriction endonucleases and topoisomerases
2 Endonucleases and polymerases
3 Restriction endonucleases and ligases
4 Peptidases and ligases
Explanation:
Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence DNA fragments generated by digestion with a restriction endonuclease can be joined together again by the enzyme ligase.
KCET - 2012
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347572
How many restriction enzymes are isolated till now?
1 90
2 9000
3 900
4 230
Explanation:
Today we know more than 900 restriction enzymes that have been isolated form over 230 strains of bacteria.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347573
Match the columns I and II. Column I Column II A Palindromic nucleotide sequences P GAATTC B Eco RI Q First endonuclease C Hind II R Site for cutting endonucleases
1 A – R , B – P , C – Q
2 A – Q , B – R , C – P
3 A – P , B – R , C – Q
4 A – Q , B – P , C – R
Explanation:
\({\rm{A - R, B - P, C - Q}}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347574
Identify the restriction enzyme used based on the restriction site
347571
The enzymes which are absolutely necessary for recombinant DNA technology are:
1 Restriction endonucleases and topoisomerases
2 Endonucleases and polymerases
3 Restriction endonucleases and ligases
4 Peptidases and ligases
Explanation:
Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence DNA fragments generated by digestion with a restriction endonuclease can be joined together again by the enzyme ligase.
KCET - 2012
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347572
How many restriction enzymes are isolated till now?
1 90
2 9000
3 900
4 230
Explanation:
Today we know more than 900 restriction enzymes that have been isolated form over 230 strains of bacteria.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347573
Match the columns I and II. Column I Column II A Palindromic nucleotide sequences P GAATTC B Eco RI Q First endonuclease C Hind II R Site for cutting endonucleases
1 A – R , B – P , C – Q
2 A – Q , B – R , C – P
3 A – P , B – R , C – Q
4 A – Q , B – P , C – R
Explanation:
\({\rm{A - R, B - P, C - Q}}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347574
Identify the restriction enzyme used based on the restriction site
347571
The enzymes which are absolutely necessary for recombinant DNA technology are:
1 Restriction endonucleases and topoisomerases
2 Endonucleases and polymerases
3 Restriction endonucleases and ligases
4 Peptidases and ligases
Explanation:
Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence DNA fragments generated by digestion with a restriction endonuclease can be joined together again by the enzyme ligase.
KCET - 2012
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347572
How many restriction enzymes are isolated till now?
1 90
2 9000
3 900
4 230
Explanation:
Today we know more than 900 restriction enzymes that have been isolated form over 230 strains of bacteria.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347573
Match the columns I and II. Column I Column II A Palindromic nucleotide sequences P GAATTC B Eco RI Q First endonuclease C Hind II R Site for cutting endonucleases
1 A – R , B – P , C – Q
2 A – Q , B – R , C – P
3 A – P , B – R , C – Q
4 A – Q , B – P , C – R
Explanation:
\({\rm{A - R, B - P, C - Q}}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347574
Identify the restriction enzyme used based on the restriction site
347571
The enzymes which are absolutely necessary for recombinant DNA technology are:
1 Restriction endonucleases and topoisomerases
2 Endonucleases and polymerases
3 Restriction endonucleases and ligases
4 Peptidases and ligases
Explanation:
Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence DNA fragments generated by digestion with a restriction endonuclease can be joined together again by the enzyme ligase.
KCET - 2012
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347572
How many restriction enzymes are isolated till now?
1 90
2 9000
3 900
4 230
Explanation:
Today we know more than 900 restriction enzymes that have been isolated form over 230 strains of bacteria.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347573
Match the columns I and II. Column I Column II A Palindromic nucleotide sequences P GAATTC B Eco RI Q First endonuclease C Hind II R Site for cutting endonucleases
1 A – R , B – P , C – Q
2 A – Q , B – R , C – P
3 A – P , B – R , C – Q
4 A – Q , B – P , C – R
Explanation:
\({\rm{A - R, B - P, C - Q}}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347574
Identify the restriction enzyme used based on the restriction site
