Tools of Biotechnology
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347567 A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1 Ligase
2 Polymerase III
3 Taq
polymerase
4 Eco
RI
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347568 Which of the following restriction enzyme produces blunt end?

1 Xho
I
2 Eco
RV
3 Sal
I
4 Hind
III
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347569 The linking of foreign gene with the plasmid DNA is possible with the enzyme _____ and is able to form ____ bonds.

1 Exonuclease, phospho-diester
2 DNA ligase, N-glycosidic
3 DNA ligase, phospho-diester
4 DNA Polymerase, phospho-diester
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347570 In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

1 5' G A A T T C 3'; 3' C T T A A G 5'
2 5' C T CA G T 3'; 3' G A G T CA 5'
3 5' G TA T T C 3'; 3' C A TAA G 5'
4 5' G A T A C T 3'; 3' C T A T G A 5'
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347567 A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1 Ligase
2 Polymerase III
3 Taq
polymerase
4 Eco
RI
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347568 Which of the following restriction enzyme produces blunt end?

1 Xho
I
2 Eco
RV
3 Sal
I
4 Hind
III
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347569 The linking of foreign gene with the plasmid DNA is possible with the enzyme _____ and is able to form ____ bonds.

1 Exonuclease, phospho-diester
2 DNA ligase, N-glycosidic
3 DNA ligase, phospho-diester
4 DNA Polymerase, phospho-diester
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347570 In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

1 5' G A A T T C 3'; 3' C T T A A G 5'
2 5' C T CA G T 3'; 3' G A G T CA 5'
3 5' G TA T T C 3'; 3' C A TAA G 5'
4 5' G A T A C T 3'; 3' C T A T G A 5'
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347567 A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1 Ligase
2 Polymerase III
3 Taq
polymerase
4 Eco
RI
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347568 Which of the following restriction enzyme produces blunt end?

1 Xho
I
2 Eco
RV
3 Sal
I
4 Hind
III
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347569 The linking of foreign gene with the plasmid DNA is possible with the enzyme _____ and is able to form ____ bonds.

1 Exonuclease, phospho-diester
2 DNA ligase, N-glycosidic
3 DNA ligase, phospho-diester
4 DNA Polymerase, phospho-diester
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347570 In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

1 5' G A A T T C 3'; 3' C T T A A G 5'
2 5' C T CA G T 3'; 3' G A G T CA 5'
3 5' G TA T T C 3'; 3' C A TAA G 5'
4 5' G A T A C T 3'; 3' C T A T G A 5'
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347567 A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1 Ligase
2 Polymerase III
3 Taq
polymerase
4 Eco
RI
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347568 Which of the following restriction enzyme produces blunt end?

1 Xho
I
2 Eco
RV
3 Sal
I
4 Hind
III
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347569 The linking of foreign gene with the plasmid DNA is possible with the enzyme _____ and is able to form ____ bonds.

1 Exonuclease, phospho-diester
2 DNA ligase, N-glycosidic
3 DNA ligase, phospho-diester
4 DNA Polymerase, phospho-diester
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347570 In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

1 5' G A A T T C 3'; 3' C T T A A G 5'
2 5' C T CA G T 3'; 3' G A G T CA 5'
3 5' G TA T T C 3'; 3' C A TAA G 5'
4 5' G A T A C T 3'; 3' C T A T G A 5'