347531
Identify the labels and in the following Agarose gel electrophoresis representation
1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
Explanation:
M- represents largest DNA particles, N represents smaller DNA.
KCET - 2020
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347532
In gel electrophoresis, DNA fragments separate according to their size through____ provided by the agarose gel.
1 Elution
2 Sieve effect
3 Spooling
4 Transformation
Explanation:
In gel electrophoresis, DNA fragments separate according to their size through sieve effect provided by the agarose gel.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347533
Which is incorrect?
1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and .
Explanation:
When same restriction enzyme is used, resultant DNA fragments will have same kind sticky ends since they identify the same sequence.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347534
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
Explanation:
The differential mobility of DNA depends upon charge & size of DNA.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347535
A mixture containing DNA fragments , and with molecular weights, and , was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:
1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B
Explanation:
Shorter lengths of DNA move faster than longer lengths so move further in the time the current is run. So, since D has the heaviest weight (implying it has the longest strand), it will tend to remain far from the anode. B being a lightest fragment moves farthest from the cathode. Hence The positions of these fragments from cathode to anode sides of the gel would be D, C, A, B.
347531
Identify the labels and in the following Agarose gel electrophoresis representation
1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
Explanation:
M- represents largest DNA particles, N represents smaller DNA.
KCET - 2020
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347532
In gel electrophoresis, DNA fragments separate according to their size through____ provided by the agarose gel.
1 Elution
2 Sieve effect
3 Spooling
4 Transformation
Explanation:
In gel electrophoresis, DNA fragments separate according to their size through sieve effect provided by the agarose gel.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347533
Which is incorrect?
1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and .
Explanation:
When same restriction enzyme is used, resultant DNA fragments will have same kind sticky ends since they identify the same sequence.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347534
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
Explanation:
The differential mobility of DNA depends upon charge & size of DNA.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347535
A mixture containing DNA fragments , and with molecular weights, and , was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:
1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B
Explanation:
Shorter lengths of DNA move faster than longer lengths so move further in the time the current is run. So, since D has the heaviest weight (implying it has the longest strand), it will tend to remain far from the anode. B being a lightest fragment moves farthest from the cathode. Hence The positions of these fragments from cathode to anode sides of the gel would be D, C, A, B.
347531
Identify the labels and in the following Agarose gel electrophoresis representation
1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
Explanation:
M- represents largest DNA particles, N represents smaller DNA.
KCET - 2020
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347532
In gel electrophoresis, DNA fragments separate according to their size through____ provided by the agarose gel.
1 Elution
2 Sieve effect
3 Spooling
4 Transformation
Explanation:
In gel electrophoresis, DNA fragments separate according to their size through sieve effect provided by the agarose gel.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347533
Which is incorrect?
1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and .
Explanation:
When same restriction enzyme is used, resultant DNA fragments will have same kind sticky ends since they identify the same sequence.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347534
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
Explanation:
The differential mobility of DNA depends upon charge & size of DNA.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347535
A mixture containing DNA fragments , and with molecular weights, and , was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:
1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B
Explanation:
Shorter lengths of DNA move faster than longer lengths so move further in the time the current is run. So, since D has the heaviest weight (implying it has the longest strand), it will tend to remain far from the anode. B being a lightest fragment moves farthest from the cathode. Hence The positions of these fragments from cathode to anode sides of the gel would be D, C, A, B.
347531
Identify the labels and in the following Agarose gel electrophoresis representation
1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
Explanation:
M- represents largest DNA particles, N represents smaller DNA.
KCET - 2020
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347532
In gel electrophoresis, DNA fragments separate according to their size through____ provided by the agarose gel.
1 Elution
2 Sieve effect
3 Spooling
4 Transformation
Explanation:
In gel electrophoresis, DNA fragments separate according to their size through sieve effect provided by the agarose gel.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347533
Which is incorrect?
1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and .
Explanation:
When same restriction enzyme is used, resultant DNA fragments will have same kind sticky ends since they identify the same sequence.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347534
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
Explanation:
The differential mobility of DNA depends upon charge & size of DNA.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347535
A mixture containing DNA fragments , and with molecular weights, and , was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:
1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B
Explanation:
Shorter lengths of DNA move faster than longer lengths so move further in the time the current is run. So, since D has the heaviest weight (implying it has the longest strand), it will tend to remain far from the anode. B being a lightest fragment moves farthest from the cathode. Hence The positions of these fragments from cathode to anode sides of the gel would be D, C, A, B.
347531
Identify the labels and in the following Agarose gel electrophoresis representation
1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
Explanation:
M- represents largest DNA particles, N represents smaller DNA.
KCET - 2020
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347532
In gel electrophoresis, DNA fragments separate according to their size through____ provided by the agarose gel.
1 Elution
2 Sieve effect
3 Spooling
4 Transformation
Explanation:
In gel electrophoresis, DNA fragments separate according to their size through sieve effect provided by the agarose gel.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347533
Which is incorrect?
1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and .
Explanation:
When same restriction enzyme is used, resultant DNA fragments will have same kind sticky ends since they identify the same sequence.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347534
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
Explanation:
The differential mobility of DNA depends upon charge & size of DNA.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347535
A mixture containing DNA fragments , and with molecular weights, and , was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:
1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B
Explanation:
Shorter lengths of DNA move faster than longer lengths so move further in the time the current is run. So, since D has the heaviest weight (implying it has the longest strand), it will tend to remain far from the anode. B being a lightest fragment moves farthest from the cathode. Hence The positions of these fragments from cathode to anode sides of the gel would be D, C, A, B.
