347523
Statement A : Ethidium bromide (EtBr) under UV radiation gives bright red coloured bands. Statement B : The cutting of DNA by restriction endonuclease resultsinthe fragmentsofDNA.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
To visualise DNA in the agarose gel, a positively charged dye ethidium bromide \((\mathrm{EtBr})\) is added in the agarose gel or electrophoresis buffer. This dye intercalates itself into the DNA backbone and gives orange fluorescence upon exposure to the UV light. Thus, DNA is visualised by exposure to UV light as orange coloured bands on the agarose gel. Statement B is correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347524
Statement A : Restriction enzymes recognize palindromic sequence. Statement B : DNA is negatively charged, so it moves towards anode under electric field
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
The palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept same. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on opposite strands. Both statements are correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347525
Upon exposure to UV radiation, DNA stained with ethidium bromide will show
1 Bright red colour
2 Bright blue colour
3 Bright yellow colour
4 Bright orange colour
Explanation:
In recombinant DNA technology the separated DNA fragments can be visualised only after staining the DNA with a substance known as ethidium bromide followed by exposure to U.V. radiation. DNA is visualised as bright orange coloured bands in ethidium bromide stained gel exposed to U.V. light.
NEET - 2023
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347526
Where would you find the smallest fragments of DNA after gel electrophoresis?
1 Near the cathode, farthest away from the wells
2 Near the anode, close to the wells
3 Sometimes near the cathode, sometimes near the anode
4 Near the anode, farthest away to the wells
Explanation:
The smallest fragments of DNA are found near the positive electrode as DNA is negatively charged. These fragments travel towards anode (farthest away from the leading wells).
347523
Statement A : Ethidium bromide (EtBr) under UV radiation gives bright red coloured bands. Statement B : The cutting of DNA by restriction endonuclease resultsinthe fragmentsofDNA.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
To visualise DNA in the agarose gel, a positively charged dye ethidium bromide \((\mathrm{EtBr})\) is added in the agarose gel or electrophoresis buffer. This dye intercalates itself into the DNA backbone and gives orange fluorescence upon exposure to the UV light. Thus, DNA is visualised by exposure to UV light as orange coloured bands on the agarose gel. Statement B is correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347524
Statement A : Restriction enzymes recognize palindromic sequence. Statement B : DNA is negatively charged, so it moves towards anode under electric field
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
The palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept same. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on opposite strands. Both statements are correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347525
Upon exposure to UV radiation, DNA stained with ethidium bromide will show
1 Bright red colour
2 Bright blue colour
3 Bright yellow colour
4 Bright orange colour
Explanation:
In recombinant DNA technology the separated DNA fragments can be visualised only after staining the DNA with a substance known as ethidium bromide followed by exposure to U.V. radiation. DNA is visualised as bright orange coloured bands in ethidium bromide stained gel exposed to U.V. light.
NEET - 2023
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347526
Where would you find the smallest fragments of DNA after gel electrophoresis?
1 Near the cathode, farthest away from the wells
2 Near the anode, close to the wells
3 Sometimes near the cathode, sometimes near the anode
4 Near the anode, farthest away to the wells
Explanation:
The smallest fragments of DNA are found near the positive electrode as DNA is negatively charged. These fragments travel towards anode (farthest away from the leading wells).
347523
Statement A : Ethidium bromide (EtBr) under UV radiation gives bright red coloured bands. Statement B : The cutting of DNA by restriction endonuclease resultsinthe fragmentsofDNA.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
To visualise DNA in the agarose gel, a positively charged dye ethidium bromide \((\mathrm{EtBr})\) is added in the agarose gel or electrophoresis buffer. This dye intercalates itself into the DNA backbone and gives orange fluorescence upon exposure to the UV light. Thus, DNA is visualised by exposure to UV light as orange coloured bands on the agarose gel. Statement B is correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347524
Statement A : Restriction enzymes recognize palindromic sequence. Statement B : DNA is negatively charged, so it moves towards anode under electric field
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
The palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept same. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on opposite strands. Both statements are correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347525
Upon exposure to UV radiation, DNA stained with ethidium bromide will show
1 Bright red colour
2 Bright blue colour
3 Bright yellow colour
4 Bright orange colour
Explanation:
In recombinant DNA technology the separated DNA fragments can be visualised only after staining the DNA with a substance known as ethidium bromide followed by exposure to U.V. radiation. DNA is visualised as bright orange coloured bands in ethidium bromide stained gel exposed to U.V. light.
NEET - 2023
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347526
Where would you find the smallest fragments of DNA after gel electrophoresis?
1 Near the cathode, farthest away from the wells
2 Near the anode, close to the wells
3 Sometimes near the cathode, sometimes near the anode
4 Near the anode, farthest away to the wells
Explanation:
The smallest fragments of DNA are found near the positive electrode as DNA is negatively charged. These fragments travel towards anode (farthest away from the leading wells).
347523
Statement A : Ethidium bromide (EtBr) under UV radiation gives bright red coloured bands. Statement B : The cutting of DNA by restriction endonuclease resultsinthe fragmentsofDNA.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
To visualise DNA in the agarose gel, a positively charged dye ethidium bromide \((\mathrm{EtBr})\) is added in the agarose gel or electrophoresis buffer. This dye intercalates itself into the DNA backbone and gives orange fluorescence upon exposure to the UV light. Thus, DNA is visualised by exposure to UV light as orange coloured bands on the agarose gel. Statement B is correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347524
Statement A : Restriction enzymes recognize palindromic sequence. Statement B : DNA is negatively charged, so it moves towards anode under electric field
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
The palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading is kept same. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on opposite strands. Both statements are correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347525
Upon exposure to UV radiation, DNA stained with ethidium bromide will show
1 Bright red colour
2 Bright blue colour
3 Bright yellow colour
4 Bright orange colour
Explanation:
In recombinant DNA technology the separated DNA fragments can be visualised only after staining the DNA with a substance known as ethidium bromide followed by exposure to U.V. radiation. DNA is visualised as bright orange coloured bands in ethidium bromide stained gel exposed to U.V. light.
NEET - 2023
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347526
Where would you find the smallest fragments of DNA after gel electrophoresis?
1 Near the cathode, farthest away from the wells
2 Near the anode, close to the wells
3 Sometimes near the cathode, sometimes near the anode
4 Near the anode, farthest away to the wells
Explanation:
The smallest fragments of DNA are found near the positive electrode as DNA is negatively charged. These fragments travel towards anode (farthest away from the leading wells).
