347514
Assertion : Ethidium bromide (EtBr) under UV radiation gives bright orange coloured bands. Reason : The separated DNA fragments can be seen after staining the DNA with compound EtBr.
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose gel electrophoresis is used to segregate DNA fragments according to the mass and size. Ethidium bromide is the fluorescent stain used in this technique. Ethidium bromide, when exposed to ultraviolet light, produces a fluorescent effect. Hence the DNA tagged by it can be traced quickly on the transparent gel. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation as we cannot see pure DNA fragments in the visible light and without staining.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347515
In gel electrophoresis :-
1 DNA fragments are arranged according to charge
2 DNA fragments are treated by alkali solution
3 DNA fragments are denatured
4 DNA fragments are arranged according to length on gel by applying electric field
Explanation:
Gel electrophoresis is a technique used for separation of DNA fragments based on their size and charge under the influence of an electric field under suitable medium.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347516
Assertion : The matrix used in gel electrophoresis should have controllable pore size Reason : Agarose concentration can be changed to change pore sizes
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose is a polysaccharide obtained from red algae. Agarose dissolves in hot water and when it is cooled, it forms gel. Pore size depends upon agarose concentration. In general, a \(1 \%(\mathrm{w} / \mathrm{v})\) gel will have a pore size of \(150 \mathrm{~nm}\). While a \(0.16 \%\) gel has pore size of \(500 \mathrm{~nm}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347517
What is the criterion of DNA fragments movement on agarose gel during gel electrophoresis?
1 The smaller the fragment size, the farther it moves
2 Positively charged fragments move to farther end
3 Negatively charged fragments do not move
4 The larger the fragments size, the farther it moves
Explanation:
During gel electrophoresis, DNA fragments separate according to their size through sieving effect provided by agarose gel.
NEET - 2017
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347518
Which statement is incorrect? I. Genetic engineering is also called recombinant DNA technology II. Bacteriophage is not used as vector III. MALAYALAM is a palindrome IV. Ethidium bromide can not be used for staining DNA
1 II, IV
2 I, II
3 I, II, III, IV
4 III, IV
Explanation:
II, IV II. Bacteriophage is used as vector. IV. Ethidium bromide is used for staining DNA
347514
Assertion : Ethidium bromide (EtBr) under UV radiation gives bright orange coloured bands. Reason : The separated DNA fragments can be seen after staining the DNA with compound EtBr.
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose gel electrophoresis is used to segregate DNA fragments according to the mass and size. Ethidium bromide is the fluorescent stain used in this technique. Ethidium bromide, when exposed to ultraviolet light, produces a fluorescent effect. Hence the DNA tagged by it can be traced quickly on the transparent gel. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation as we cannot see pure DNA fragments in the visible light and without staining.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347515
In gel electrophoresis :-
1 DNA fragments are arranged according to charge
2 DNA fragments are treated by alkali solution
3 DNA fragments are denatured
4 DNA fragments are arranged according to length on gel by applying electric field
Explanation:
Gel electrophoresis is a technique used for separation of DNA fragments based on their size and charge under the influence of an electric field under suitable medium.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347516
Assertion : The matrix used in gel electrophoresis should have controllable pore size Reason : Agarose concentration can be changed to change pore sizes
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose is a polysaccharide obtained from red algae. Agarose dissolves in hot water and when it is cooled, it forms gel. Pore size depends upon agarose concentration. In general, a \(1 \%(\mathrm{w} / \mathrm{v})\) gel will have a pore size of \(150 \mathrm{~nm}\). While a \(0.16 \%\) gel has pore size of \(500 \mathrm{~nm}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347517
What is the criterion of DNA fragments movement on agarose gel during gel electrophoresis?
1 The smaller the fragment size, the farther it moves
2 Positively charged fragments move to farther end
3 Negatively charged fragments do not move
4 The larger the fragments size, the farther it moves
Explanation:
During gel electrophoresis, DNA fragments separate according to their size through sieving effect provided by agarose gel.
NEET - 2017
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347518
Which statement is incorrect? I. Genetic engineering is also called recombinant DNA technology II. Bacteriophage is not used as vector III. MALAYALAM is a palindrome IV. Ethidium bromide can not be used for staining DNA
1 II, IV
2 I, II
3 I, II, III, IV
4 III, IV
Explanation:
II, IV II. Bacteriophage is used as vector. IV. Ethidium bromide is used for staining DNA
NEET Test Series from KOTA - 10 Papers In MS WORD
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BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347514
Assertion : Ethidium bromide (EtBr) under UV radiation gives bright orange coloured bands. Reason : The separated DNA fragments can be seen after staining the DNA with compound EtBr.
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose gel electrophoresis is used to segregate DNA fragments according to the mass and size. Ethidium bromide is the fluorescent stain used in this technique. Ethidium bromide, when exposed to ultraviolet light, produces a fluorescent effect. Hence the DNA tagged by it can be traced quickly on the transparent gel. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation as we cannot see pure DNA fragments in the visible light and without staining.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347515
In gel electrophoresis :-
1 DNA fragments are arranged according to charge
2 DNA fragments are treated by alkali solution
3 DNA fragments are denatured
4 DNA fragments are arranged according to length on gel by applying electric field
Explanation:
Gel electrophoresis is a technique used for separation of DNA fragments based on their size and charge under the influence of an electric field under suitable medium.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347516
Assertion : The matrix used in gel electrophoresis should have controllable pore size Reason : Agarose concentration can be changed to change pore sizes
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose is a polysaccharide obtained from red algae. Agarose dissolves in hot water and when it is cooled, it forms gel. Pore size depends upon agarose concentration. In general, a \(1 \%(\mathrm{w} / \mathrm{v})\) gel will have a pore size of \(150 \mathrm{~nm}\). While a \(0.16 \%\) gel has pore size of \(500 \mathrm{~nm}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347517
What is the criterion of DNA fragments movement on agarose gel during gel electrophoresis?
1 The smaller the fragment size, the farther it moves
2 Positively charged fragments move to farther end
3 Negatively charged fragments do not move
4 The larger the fragments size, the farther it moves
Explanation:
During gel electrophoresis, DNA fragments separate according to their size through sieving effect provided by agarose gel.
NEET - 2017
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347518
Which statement is incorrect? I. Genetic engineering is also called recombinant DNA technology II. Bacteriophage is not used as vector III. MALAYALAM is a palindrome IV. Ethidium bromide can not be used for staining DNA
1 II, IV
2 I, II
3 I, II, III, IV
4 III, IV
Explanation:
II, IV II. Bacteriophage is used as vector. IV. Ethidium bromide is used for staining DNA
347514
Assertion : Ethidium bromide (EtBr) under UV radiation gives bright orange coloured bands. Reason : The separated DNA fragments can be seen after staining the DNA with compound EtBr.
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose gel electrophoresis is used to segregate DNA fragments according to the mass and size. Ethidium bromide is the fluorescent stain used in this technique. Ethidium bromide, when exposed to ultraviolet light, produces a fluorescent effect. Hence the DNA tagged by it can be traced quickly on the transparent gel. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation as we cannot see pure DNA fragments in the visible light and without staining.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347515
In gel electrophoresis :-
1 DNA fragments are arranged according to charge
2 DNA fragments are treated by alkali solution
3 DNA fragments are denatured
4 DNA fragments are arranged according to length on gel by applying electric field
Explanation:
Gel electrophoresis is a technique used for separation of DNA fragments based on their size and charge under the influence of an electric field under suitable medium.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347516
Assertion : The matrix used in gel electrophoresis should have controllable pore size Reason : Agarose concentration can be changed to change pore sizes
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose is a polysaccharide obtained from red algae. Agarose dissolves in hot water and when it is cooled, it forms gel. Pore size depends upon agarose concentration. In general, a \(1 \%(\mathrm{w} / \mathrm{v})\) gel will have a pore size of \(150 \mathrm{~nm}\). While a \(0.16 \%\) gel has pore size of \(500 \mathrm{~nm}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347517
What is the criterion of DNA fragments movement on agarose gel during gel electrophoresis?
1 The smaller the fragment size, the farther it moves
2 Positively charged fragments move to farther end
3 Negatively charged fragments do not move
4 The larger the fragments size, the farther it moves
Explanation:
During gel electrophoresis, DNA fragments separate according to their size through sieving effect provided by agarose gel.
NEET - 2017
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347518
Which statement is incorrect? I. Genetic engineering is also called recombinant DNA technology II. Bacteriophage is not used as vector III. MALAYALAM is a palindrome IV. Ethidium bromide can not be used for staining DNA
1 II, IV
2 I, II
3 I, II, III, IV
4 III, IV
Explanation:
II, IV II. Bacteriophage is used as vector. IV. Ethidium bromide is used for staining DNA
347514
Assertion : Ethidium bromide (EtBr) under UV radiation gives bright orange coloured bands. Reason : The separated DNA fragments can be seen after staining the DNA with compound EtBr.
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose gel electrophoresis is used to segregate DNA fragments according to the mass and size. Ethidium bromide is the fluorescent stain used in this technique. Ethidium bromide, when exposed to ultraviolet light, produces a fluorescent effect. Hence the DNA tagged by it can be traced quickly on the transparent gel. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation as we cannot see pure DNA fragments in the visible light and without staining.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347515
In gel electrophoresis :-
1 DNA fragments are arranged according to charge
2 DNA fragments are treated by alkali solution
3 DNA fragments are denatured
4 DNA fragments are arranged according to length on gel by applying electric field
Explanation:
Gel electrophoresis is a technique used for separation of DNA fragments based on their size and charge under the influence of an electric field under suitable medium.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347516
Assertion : The matrix used in gel electrophoresis should have controllable pore size Reason : Agarose concentration can be changed to change pore sizes
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Agarose is a polysaccharide obtained from red algae. Agarose dissolves in hot water and when it is cooled, it forms gel. Pore size depends upon agarose concentration. In general, a \(1 \%(\mathrm{w} / \mathrm{v})\) gel will have a pore size of \(150 \mathrm{~nm}\). While a \(0.16 \%\) gel has pore size of \(500 \mathrm{~nm}\)
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347517
What is the criterion of DNA fragments movement on agarose gel during gel electrophoresis?
1 The smaller the fragment size, the farther it moves
2 Positively charged fragments move to farther end
3 Negatively charged fragments do not move
4 The larger the fragments size, the farther it moves
Explanation:
During gel electrophoresis, DNA fragments separate according to their size through sieving effect provided by agarose gel.
NEET - 2017
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347518
Which statement is incorrect? I. Genetic engineering is also called recombinant DNA technology II. Bacteriophage is not used as vector III. MALAYALAM is a palindrome IV. Ethidium bromide can not be used for staining DNA
1 II, IV
2 I, II
3 I, II, III, IV
4 III, IV
Explanation:
II, IV II. Bacteriophage is used as vector. IV. Ethidium bromide is used for staining DNA