Tools of Biotechnology
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347548 Which is incorrect?

1 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 Making multiple identical copies of any template DNA is called cloning.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347622 Which of the following restriction endonuclease is obtained from
E.coli
?

1 ECOrI
2 EcoRI
3 ECORi
4 ECORI
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347513 Identify \(\mathrm{X}\) and also the technique depicted in the picture
supporting img

1 X-wells, Transformation
2 X-wells, Gel electrophoresis
3 X-anode, Transformation
4 X-anode, Gel electrophoresis
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347503 Select the correct answer
A. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the optimal conditions for that specific enzyme.
B. Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion.

1 Only A is correct
2 Only B is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347504 With respect to DNA fragmentation:
Statement A :
Gel electrophoresis and elution are two important processes.
Statement B :
After staining with ethidium bromide it has to be exposed to U.V. light.

1 Both Statement A and Statement B are correct.
2 Only Statement A is correct and Statement B is not correct.
3 Only Statement A is correct.
4 Only Statement B is correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347548 Which is incorrect?

1 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 Making multiple identical copies of any template DNA is called cloning.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347622 Which of the following restriction endonuclease is obtained from
E.coli
?

1 ECOrI
2 EcoRI
3 ECORi
4 ECORI
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347513 Identify \(\mathrm{X}\) and also the technique depicted in the picture
supporting img

1 X-wells, Transformation
2 X-wells, Gel electrophoresis
3 X-anode, Transformation
4 X-anode, Gel electrophoresis
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347503 Select the correct answer
A. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the optimal conditions for that specific enzyme.
B. Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion.

1 Only A is correct
2 Only B is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347504 With respect to DNA fragmentation:
Statement A :
Gel electrophoresis and elution are two important processes.
Statement B :
After staining with ethidium bromide it has to be exposed to U.V. light.

1 Both Statement A and Statement B are correct.
2 Only Statement A is correct and Statement B is not correct.
3 Only Statement A is correct.
4 Only Statement B is correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347548 Which is incorrect?

1 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 Making multiple identical copies of any template DNA is called cloning.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347622 Which of the following restriction endonuclease is obtained from
E.coli
?

1 ECOrI
2 EcoRI
3 ECORi
4 ECORI
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347513 Identify \(\mathrm{X}\) and also the technique depicted in the picture
supporting img

1 X-wells, Transformation
2 X-wells, Gel electrophoresis
3 X-anode, Transformation
4 X-anode, Gel electrophoresis
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347503 Select the correct answer
A. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the optimal conditions for that specific enzyme.
B. Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion.

1 Only A is correct
2 Only B is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347504 With respect to DNA fragmentation:
Statement A :
Gel electrophoresis and elution are two important processes.
Statement B :
After staining with ethidium bromide it has to be exposed to U.V. light.

1 Both Statement A and Statement B are correct.
2 Only Statement A is correct and Statement B is not correct.
3 Only Statement A is correct.
4 Only Statement B is correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347548 Which is incorrect?

1 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 Making multiple identical copies of any template DNA is called cloning.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347622 Which of the following restriction endonuclease is obtained from
E.coli
?

1 ECOrI
2 EcoRI
3 ECORi
4 ECORI
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347513 Identify \(\mathrm{X}\) and also the technique depicted in the picture
supporting img

1 X-wells, Transformation
2 X-wells, Gel electrophoresis
3 X-anode, Transformation
4 X-anode, Gel electrophoresis
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347503 Select the correct answer
A. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the optimal conditions for that specific enzyme.
B. Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion.

1 Only A is correct
2 Only B is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347504 With respect to DNA fragmentation:
Statement A :
Gel electrophoresis and elution are two important processes.
Statement B :
After staining with ethidium bromide it has to be exposed to U.V. light.

1 Both Statement A and Statement B are correct.
2 Only Statement A is correct and Statement B is not correct.
3 Only Statement A is correct.
4 Only Statement B is correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347548 Which is incorrect?

1 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 Making multiple identical copies of any template DNA is called cloning.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347622 Which of the following restriction endonuclease is obtained from
E.coli
?

1 ECOrI
2 EcoRI
3 ECORi
4 ECORI
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347513 Identify \(\mathrm{X}\) and also the technique depicted in the picture
supporting img

1 X-wells, Transformation
2 X-wells, Gel electrophoresis
3 X-anode, Transformation
4 X-anode, Gel electrophoresis
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347503 Select the correct answer
A. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzyme, at the optimal conditions for that specific enzyme.
B. Agarose gel electrophoresis is employed to check the progression of a restriction enzyme digestion.

1 Only A is correct
2 Only B is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347504 With respect to DNA fragmentation:
Statement A :
Gel electrophoresis and elution are two important processes.
Statement B :
After staining with ethidium bromide it has to be exposed to U.V. light.

1 Both Statement A and Statement B are correct.
2 Only Statement A is correct and Statement B is not correct.
3 Only Statement A is correct.
4 Only Statement B is correct.