Tools of Biotechnology
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347531 Identify the labels \(\mathrm{M}\) and \(\mathrm{N}\) in the following Agarose gel electrophoresis representation
supporting img

1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, \(\mathrm{N}\) - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347532 In gel electrophoresis, DNA fragments separate according to their size through____
provided by the agarose gel.

1 Elution
2 Sieve effect
3 Spooling
4 Transformation
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347533 Which is incorrect?

1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347534 In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347535 A mixture containing DNA fragments \(A, B, C\), and \(\mathrm{D}\) with molecular weights, \(\mathrm{A}+\mathrm{B}=\mathrm{C}, \mathrm{A}>\mathrm{B}\) and \(\mathrm{D}>\mathrm{C}\), was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:

1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347531 Identify the labels \(\mathrm{M}\) and \(\mathrm{N}\) in the following Agarose gel electrophoresis representation
supporting img

1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, \(\mathrm{N}\) - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347532 In gel electrophoresis, DNA fragments separate according to their size through____
provided by the agarose gel.

1 Elution
2 Sieve effect
3 Spooling
4 Transformation
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347533 Which is incorrect?

1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347534 In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347535 A mixture containing DNA fragments \(A, B, C\), and \(\mathrm{D}\) with molecular weights, \(\mathrm{A}+\mathrm{B}=\mathrm{C}, \mathrm{A}>\mathrm{B}\) and \(\mathrm{D}>\mathrm{C}\), was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:

1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347531 Identify the labels \(\mathrm{M}\) and \(\mathrm{N}\) in the following Agarose gel electrophoresis representation
supporting img

1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, \(\mathrm{N}\) - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347532 In gel electrophoresis, DNA fragments separate according to their size through____
provided by the agarose gel.

1 Elution
2 Sieve effect
3 Spooling
4 Transformation
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347533 Which is incorrect?

1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347534 In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347535 A mixture containing DNA fragments \(A, B, C\), and \(\mathrm{D}\) with molecular weights, \(\mathrm{A}+\mathrm{B}=\mathrm{C}, \mathrm{A}>\mathrm{B}\) and \(\mathrm{D}>\mathrm{C}\), was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:

1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B
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BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347531 Identify the labels \(\mathrm{M}\) and \(\mathrm{N}\) in the following Agarose gel electrophoresis representation
supporting img

1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, \(\mathrm{N}\) - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347532 In gel electrophoresis, DNA fragments separate according to their size through____
provided by the agarose gel.

1 Elution
2 Sieve effect
3 Spooling
4 Transformation
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347533 Which is incorrect?

1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347534 In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347535 A mixture containing DNA fragments \(A, B, C\), and \(\mathrm{D}\) with molecular weights, \(\mathrm{A}+\mathrm{B}=\mathrm{C}, \mathrm{A}>\mathrm{B}\) and \(\mathrm{D}>\mathrm{C}\), was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:

1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347531 Identify the labels \(\mathrm{M}\) and \(\mathrm{N}\) in the following Agarose gel electrophoresis representation
supporting img

1 M - Digested DNA bands, N - Undigested DNA bands
2 M - Hybridized DNA bands, \(\mathrm{N}\) - Unhybridised DNA bands.
3 M-Largest DNA bands, N - Smallest DNA bands.
4 M - Smallest DNA bands, N - Largest DNA bands.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347532 In gel electrophoresis, DNA fragments separate according to their size through____
provided by the agarose gel.

1 Elution
2 Sieve effect
3 Spooling
4 Transformation
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347533 Which is incorrect?

1 Separated DNA fragments can be visualized only after staining the DNA with Ethidium bromide and exposure to UV light
2 Each restriction endonuclease recognizes a specific palindromic nucleotide sequences in DNA.
3 When cut by same restriction enzyme, the resultant DNA fragments do not have the same kind of 'sticky-ends'.
4 EcoRI cuts the DNA between bases G and \(\mathrm{A}\).
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347534 In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

1 Charge only
2 Size only
3 Charge to size ratio
4 All of the above
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347535 A mixture containing DNA fragments \(A, B, C\), and \(\mathrm{D}\) with molecular weights, \(\mathrm{A}+\mathrm{B}=\mathrm{C}, \mathrm{A}>\mathrm{B}\) and \(\mathrm{D}>\mathrm{C}\), was subjected to agarose gel electrophoresis. The position of these fragments from anode to cathode sides of gel should be:

1 A, B, C, D
2 B, A, C, D
3 B, A, D, C
4 C, D, A, B