347441
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 Annealing
2 Extension
3 Denaturation
4 Ligation
Explanation:
The initial denaturation step is commonly performed at \(94-98^{\circ} \mathrm{C}\) for \(1-3\) minutes.
NEET - 2021
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347442
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
1 Easy availability of DNA template
2 Availability of synthetic primers
3 Availability of cheapdeoxyribonucleotides
4 Availability of 'Thermostable' DNA polymerase
Explanation:
Thermostable DNA polymerase is isolated from a bacterium Thermus aquaticus popularises PCR, because this enzyme remains active during high temperature.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347443
Statement A : PCR technique is helpful in detecting bacterial and viral diseases even when symptoms of disease are not yet visible Statement B : PCR is helpful in the identification of the coronavirus.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
The technique of polymerase chain reaction (PCR) has been applied to detect HIV in suspected AIDS patients and to detect mutations in genes in suspected cancer patients. Both statements are correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347444
Primers are
1 Specific sequences that serve as restriction sites
2 Chemically synthesised oligonucleotides that are not complementary to the region of template DNA
3 The double-stranded DNA that need to be amplified
4 Small chemically synthesied oligonucleotides that are complementary to the region of template DNA
Explanation:
Primers are small chemically synthesied oligonucleotides that are complementary the region of template DNA.
347441
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 Annealing
2 Extension
3 Denaturation
4 Ligation
Explanation:
The initial denaturation step is commonly performed at \(94-98^{\circ} \mathrm{C}\) for \(1-3\) minutes.
NEET - 2021
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347442
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
1 Easy availability of DNA template
2 Availability of synthetic primers
3 Availability of cheapdeoxyribonucleotides
4 Availability of 'Thermostable' DNA polymerase
Explanation:
Thermostable DNA polymerase is isolated from a bacterium Thermus aquaticus popularises PCR, because this enzyme remains active during high temperature.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347443
Statement A : PCR technique is helpful in detecting bacterial and viral diseases even when symptoms of disease are not yet visible Statement B : PCR is helpful in the identification of the coronavirus.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
The technique of polymerase chain reaction (PCR) has been applied to detect HIV in suspected AIDS patients and to detect mutations in genes in suspected cancer patients. Both statements are correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347444
Primers are
1 Specific sequences that serve as restriction sites
2 Chemically synthesised oligonucleotides that are not complementary to the region of template DNA
3 The double-stranded DNA that need to be amplified
4 Small chemically synthesied oligonucleotides that are complementary to the region of template DNA
Explanation:
Primers are small chemically synthesied oligonucleotides that are complementary the region of template DNA.
347441
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 Annealing
2 Extension
3 Denaturation
4 Ligation
Explanation:
The initial denaturation step is commonly performed at \(94-98^{\circ} \mathrm{C}\) for \(1-3\) minutes.
NEET - 2021
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347442
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
1 Easy availability of DNA template
2 Availability of synthetic primers
3 Availability of cheapdeoxyribonucleotides
4 Availability of 'Thermostable' DNA polymerase
Explanation:
Thermostable DNA polymerase is isolated from a bacterium Thermus aquaticus popularises PCR, because this enzyme remains active during high temperature.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347443
Statement A : PCR technique is helpful in detecting bacterial and viral diseases even when symptoms of disease are not yet visible Statement B : PCR is helpful in the identification of the coronavirus.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
The technique of polymerase chain reaction (PCR) has been applied to detect HIV in suspected AIDS patients and to detect mutations in genes in suspected cancer patients. Both statements are correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347444
Primers are
1 Specific sequences that serve as restriction sites
2 Chemically synthesised oligonucleotides that are not complementary to the region of template DNA
3 The double-stranded DNA that need to be amplified
4 Small chemically synthesied oligonucleotides that are complementary to the region of template DNA
Explanation:
Primers are small chemically synthesied oligonucleotides that are complementary the region of template DNA.
347441
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 Annealing
2 Extension
3 Denaturation
4 Ligation
Explanation:
The initial denaturation step is commonly performed at \(94-98^{\circ} \mathrm{C}\) for \(1-3\) minutes.
NEET - 2021
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347442
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
1 Easy availability of DNA template
2 Availability of synthetic primers
3 Availability of cheapdeoxyribonucleotides
4 Availability of 'Thermostable' DNA polymerase
Explanation:
Thermostable DNA polymerase is isolated from a bacterium Thermus aquaticus popularises PCR, because this enzyme remains active during high temperature.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347443
Statement A : PCR technique is helpful in detecting bacterial and viral diseases even when symptoms of disease are not yet visible Statement B : PCR is helpful in the identification of the coronavirus.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
The technique of polymerase chain reaction (PCR) has been applied to detect HIV in suspected AIDS patients and to detect mutations in genes in suspected cancer patients. Both statements are correct.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347444
Primers are
1 Specific sequences that serve as restriction sites
2 Chemically synthesised oligonucleotides that are not complementary to the region of template DNA
3 The double-stranded DNA that need to be amplified
4 Small chemically synthesied oligonucleotides that are complementary to the region of template DNA
Explanation:
Primers are small chemically synthesied oligonucleotides that are complementary the region of template DNA.
