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BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347378 The technique of bombarding plant cells with high velocity microparticles of gold or tungsten, coated with DNA, is :

1 Microinjection
2 Biolistic method
3 Heat shock method
4 By disarmed pathogen vector
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347379 Which of the statements given is incorrect?

1 In microinjection method, foreign DNA is directly injected into the nucleus of animal cell
2 Agrobacterium tumefaciens
is a natural genetic engineer
3 Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
4 Restriction enzymes belong to a class of enzymes called nucleases.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347380 Select the correct answer
I. Introduction of recombinant DNA in bacteria is by treatment on ice, followed by placing them briefly at \(42^{\circ} \mathrm{C}\) (heat shock), and then putting them back on ice.
II. In micro-injection, recombinant DNA is directly injected into the cytoplasm of an animal cell.

1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347381 Biolistics method is suitable for gene transfer into

1 Viruses
2 Animal cells
3 Bacteria
4 Plant cells
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347378 The technique of bombarding plant cells with high velocity microparticles of gold or tungsten, coated with DNA, is :

1 Microinjection
2 Biolistic method
3 Heat shock method
4 By disarmed pathogen vector
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347379 Which of the statements given is incorrect?

1 In microinjection method, foreign DNA is directly injected into the nucleus of animal cell
2 Agrobacterium tumefaciens
is a natural genetic engineer
3 Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
4 Restriction enzymes belong to a class of enzymes called nucleases.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347380 Select the correct answer
I. Introduction of recombinant DNA in bacteria is by treatment on ice, followed by placing them briefly at \(42^{\circ} \mathrm{C}\) (heat shock), and then putting them back on ice.
II. In micro-injection, recombinant DNA is directly injected into the cytoplasm of an animal cell.

1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347381 Biolistics method is suitable for gene transfer into

1 Viruses
2 Animal cells
3 Bacteria
4 Plant cells
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347378 The technique of bombarding plant cells with high velocity microparticles of gold or tungsten, coated with DNA, is :

1 Microinjection
2 Biolistic method
3 Heat shock method
4 By disarmed pathogen vector
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347379 Which of the statements given is incorrect?

1 In microinjection method, foreign DNA is directly injected into the nucleus of animal cell
2 Agrobacterium tumefaciens
is a natural genetic engineer
3 Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
4 Restriction enzymes belong to a class of enzymes called nucleases.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347380 Select the correct answer
I. Introduction of recombinant DNA in bacteria is by treatment on ice, followed by placing them briefly at \(42^{\circ} \mathrm{C}\) (heat shock), and then putting them back on ice.
II. In micro-injection, recombinant DNA is directly injected into the cytoplasm of an animal cell.

1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347381 Biolistics method is suitable for gene transfer into

1 Viruses
2 Animal cells
3 Bacteria
4 Plant cells
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347378 The technique of bombarding plant cells with high velocity microparticles of gold or tungsten, coated with DNA, is :

1 Microinjection
2 Biolistic method
3 Heat shock method
4 By disarmed pathogen vector
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347379 Which of the statements given is incorrect?

1 In microinjection method, foreign DNA is directly injected into the nucleus of animal cell
2 Agrobacterium tumefaciens
is a natural genetic engineer
3 Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
4 Restriction enzymes belong to a class of enzymes called nucleases.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347380 Select the correct answer
I. Introduction of recombinant DNA in bacteria is by treatment on ice, followed by placing them briefly at \(42^{\circ} \mathrm{C}\) (heat shock), and then putting them back on ice.
II. In micro-injection, recombinant DNA is directly injected into the cytoplasm of an animal cell.

1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347381 Biolistics method is suitable for gene transfer into

1 Viruses
2 Animal cells
3 Bacteria
4 Plant cells