347369
Assertion : Bacteriophages because of their high number per cell, have very high copy numbers of their genome within the bacterial cells. Reason : Therefore they are used as vectors
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Both are correct and the reason is the correct explanation of the assertion.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347370
Recombinant DNA is forced to enter the host cells by incubating the cells with rDNA is?
1 On ice followed by heat shock and then again on ice
2 In Ethidium bromide followed by calcium salts
3 Into chilled ethanol followed by ice and then into \(\mathrm{CsCl}\)
4 Heat followed by ice and then again on ice
Explanation:
A bacterial cell can be made competent to receive recombinant DNA by incubating on ice along with rDNA followed by heat \(\left(42^{\circ} \mathrm{C}\right)\) shock and then again on ice.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347371
Assertion : In recombinant DNA technology, human genes are always transferred into bacteria (Prokaryotes) or yeast (eukaryotes) Reason : Both bacteria and yeast multiply very fast to form huge populations which express the desired gene
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
In recombinant DNA technology, a specific DNA molecule is inserted into a vector DNA molecule that can carry foreign DNA, and the resulting recombinant DNA is introduced into a host organism (often the common bacterium Escherichia coli or the yeast Saccharomyces cerevisiae). Large numbers of genetically identical host organisms which carrying the same specific foreign DNA molecule can be produced as they multiply rapidly and allow the DNA or its protein product to be produced in large quantities. Most often bacteria are used as the host organism, because of their fast growth and the ready availability of techniques for manipulating and growing bacteria in small- and large-scale cultures
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347372
Which of the following method(s) is used to introduce foreign DNA into plant host cells?
1 Gene gun method
2 Biolistic method
3 Elution
4 Both 1 and 2
Explanation:
Vectors, gene gun (biolistic) and microinjection are all viable techniques to insert foreign DNA into the host cell. So, the correct option is both \(1 \& 2\).
347369
Assertion : Bacteriophages because of their high number per cell, have very high copy numbers of their genome within the bacterial cells. Reason : Therefore they are used as vectors
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Both are correct and the reason is the correct explanation of the assertion.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347370
Recombinant DNA is forced to enter the host cells by incubating the cells with rDNA is?
1 On ice followed by heat shock and then again on ice
2 In Ethidium bromide followed by calcium salts
3 Into chilled ethanol followed by ice and then into \(\mathrm{CsCl}\)
4 Heat followed by ice and then again on ice
Explanation:
A bacterial cell can be made competent to receive recombinant DNA by incubating on ice along with rDNA followed by heat \(\left(42^{\circ} \mathrm{C}\right)\) shock and then again on ice.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347371
Assertion : In recombinant DNA technology, human genes are always transferred into bacteria (Prokaryotes) or yeast (eukaryotes) Reason : Both bacteria and yeast multiply very fast to form huge populations which express the desired gene
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
In recombinant DNA technology, a specific DNA molecule is inserted into a vector DNA molecule that can carry foreign DNA, and the resulting recombinant DNA is introduced into a host organism (often the common bacterium Escherichia coli or the yeast Saccharomyces cerevisiae). Large numbers of genetically identical host organisms which carrying the same specific foreign DNA molecule can be produced as they multiply rapidly and allow the DNA or its protein product to be produced in large quantities. Most often bacteria are used as the host organism, because of their fast growth and the ready availability of techniques for manipulating and growing bacteria in small- and large-scale cultures
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347372
Which of the following method(s) is used to introduce foreign DNA into plant host cells?
1 Gene gun method
2 Biolistic method
3 Elution
4 Both 1 and 2
Explanation:
Vectors, gene gun (biolistic) and microinjection are all viable techniques to insert foreign DNA into the host cell. So, the correct option is both \(1 \& 2\).
347369
Assertion : Bacteriophages because of their high number per cell, have very high copy numbers of their genome within the bacterial cells. Reason : Therefore they are used as vectors
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Both are correct and the reason is the correct explanation of the assertion.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347370
Recombinant DNA is forced to enter the host cells by incubating the cells with rDNA is?
1 On ice followed by heat shock and then again on ice
2 In Ethidium bromide followed by calcium salts
3 Into chilled ethanol followed by ice and then into \(\mathrm{CsCl}\)
4 Heat followed by ice and then again on ice
Explanation:
A bacterial cell can be made competent to receive recombinant DNA by incubating on ice along with rDNA followed by heat \(\left(42^{\circ} \mathrm{C}\right)\) shock and then again on ice.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347371
Assertion : In recombinant DNA technology, human genes are always transferred into bacteria (Prokaryotes) or yeast (eukaryotes) Reason : Both bacteria and yeast multiply very fast to form huge populations which express the desired gene
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
In recombinant DNA technology, a specific DNA molecule is inserted into a vector DNA molecule that can carry foreign DNA, and the resulting recombinant DNA is introduced into a host organism (often the common bacterium Escherichia coli or the yeast Saccharomyces cerevisiae). Large numbers of genetically identical host organisms which carrying the same specific foreign DNA molecule can be produced as they multiply rapidly and allow the DNA or its protein product to be produced in large quantities. Most often bacteria are used as the host organism, because of their fast growth and the ready availability of techniques for manipulating and growing bacteria in small- and large-scale cultures
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347372
Which of the following method(s) is used to introduce foreign DNA into plant host cells?
1 Gene gun method
2 Biolistic method
3 Elution
4 Both 1 and 2
Explanation:
Vectors, gene gun (biolistic) and microinjection are all viable techniques to insert foreign DNA into the host cell. So, the correct option is both \(1 \& 2\).
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BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347369
Assertion : Bacteriophages because of their high number per cell, have very high copy numbers of their genome within the bacterial cells. Reason : Therefore they are used as vectors
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
Both are correct and the reason is the correct explanation of the assertion.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347370
Recombinant DNA is forced to enter the host cells by incubating the cells with rDNA is?
1 On ice followed by heat shock and then again on ice
2 In Ethidium bromide followed by calcium salts
3 Into chilled ethanol followed by ice and then into \(\mathrm{CsCl}\)
4 Heat followed by ice and then again on ice
Explanation:
A bacterial cell can be made competent to receive recombinant DNA by incubating on ice along with rDNA followed by heat \(\left(42^{\circ} \mathrm{C}\right)\) shock and then again on ice.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347371
Assertion : In recombinant DNA technology, human genes are always transferred into bacteria (Prokaryotes) or yeast (eukaryotes) Reason : Both bacteria and yeast multiply very fast to form huge populations which express the desired gene
1 Both Assertion and Reason are correct and Reason is the correct explanation of the Assertion.
2 Both Assertion and Reason are correct but Reason is not the correct explanation of the Assertion.
3 Assertion is correct but Reason is incorrect.
4 Both Assertion and Reason are incorrect
Explanation:
In recombinant DNA technology, a specific DNA molecule is inserted into a vector DNA molecule that can carry foreign DNA, and the resulting recombinant DNA is introduced into a host organism (often the common bacterium Escherichia coli or the yeast Saccharomyces cerevisiae). Large numbers of genetically identical host organisms which carrying the same specific foreign DNA molecule can be produced as they multiply rapidly and allow the DNA or its protein product to be produced in large quantities. Most often bacteria are used as the host organism, because of their fast growth and the ready availability of techniques for manipulating and growing bacteria in small- and large-scale cultures
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347372
Which of the following method(s) is used to introduce foreign DNA into plant host cells?
1 Gene gun method
2 Biolistic method
3 Elution
4 Both 1 and 2
Explanation:
Vectors, gene gun (biolistic) and microinjection are all viable techniques to insert foreign DNA into the host cell. So, the correct option is both \(1 \& 2\).
