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BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347378 The technique of bombarding plant cells with high velocity microparticles of gold or tungsten, coated with DNA, is :

1 Microinjection
2 Biolistic method
3 Heat shock method
4 By disarmed pathogen vector
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347379 Which of the statements given is incorrect?

1 In microinjection method, foreign DNA is directly injected into the nucleus of animal cell
2 Agrobacterium tumefaciens
is a natural genetic engineer
3 Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
4 Restriction enzymes belong to a class of enzymes called nucleases.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347380 Select the correct answer
I. Introduction of recombinant DNA in bacteria is by treatment on ice, followed by placing them briefly at 42C (heat shock), and then putting them back on ice.
II. In micro-injection, recombinant DNA is directly injected into the cytoplasm of an animal cell.

1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347381 Biolistics method is suitable for gene transfer into

1 Viruses
2 Animal cells
3 Bacteria
4 Plant cells
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347378 The technique of bombarding plant cells with high velocity microparticles of gold or tungsten, coated with DNA, is :

1 Microinjection
2 Biolistic method
3 Heat shock method
4 By disarmed pathogen vector
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347379 Which of the statements given is incorrect?

1 In microinjection method, foreign DNA is directly injected into the nucleus of animal cell
2 Agrobacterium tumefaciens
is a natural genetic engineer
3 Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
4 Restriction enzymes belong to a class of enzymes called nucleases.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347380 Select the correct answer
I. Introduction of recombinant DNA in bacteria is by treatment on ice, followed by placing them briefly at 42C (heat shock), and then putting them back on ice.
II. In micro-injection, recombinant DNA is directly injected into the cytoplasm of an animal cell.

1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347381 Biolistics method is suitable for gene transfer into

1 Viruses
2 Animal cells
3 Bacteria
4 Plant cells
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347378 The technique of bombarding plant cells with high velocity microparticles of gold or tungsten, coated with DNA, is :

1 Microinjection
2 Biolistic method
3 Heat shock method
4 By disarmed pathogen vector
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347379 Which of the statements given is incorrect?

1 In microinjection method, foreign DNA is directly injected into the nucleus of animal cell
2 Agrobacterium tumefaciens
is a natural genetic engineer
3 Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
4 Restriction enzymes belong to a class of enzymes called nucleases.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347380 Select the correct answer
I. Introduction of recombinant DNA in bacteria is by treatment on ice, followed by placing them briefly at 42C (heat shock), and then putting them back on ice.
II. In micro-injection, recombinant DNA is directly injected into the cytoplasm of an animal cell.

1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347381 Biolistics method is suitable for gene transfer into

1 Viruses
2 Animal cells
3 Bacteria
4 Plant cells
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347378 The technique of bombarding plant cells with high velocity microparticles of gold or tungsten, coated with DNA, is :

1 Microinjection
2 Biolistic method
3 Heat shock method
4 By disarmed pathogen vector
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347379 Which of the statements given is incorrect?

1 In microinjection method, foreign DNA is directly injected into the nucleus of animal cell
2 Agrobacterium tumefaciens
is a natural genetic engineer
3 Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
4 Restriction enzymes belong to a class of enzymes called nucleases.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347380 Select the correct answer
I. Introduction of recombinant DNA in bacteria is by treatment on ice, followed by placing them briefly at 42C (heat shock), and then putting them back on ice.
II. In micro-injection, recombinant DNA is directly injected into the cytoplasm of an animal cell.

1 Only I is correct
2 Only II is correct
3 Both are correct
4 None are correct
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347381 Biolistics method is suitable for gene transfer into

1 Viruses
2 Animal cells
3 Bacteria
4 Plant cells