347271
An antibiotic resistance gene in a vector usually helps in the selection of:
1 Competent bacterial cells
2 Transformed bacterial cells
3 Recombinant bacterial cells
4 None of the above
Explanation:
As a result only bacteria containing a plasmid with antibiotic resistance will grow in the presence of antibiotics. This gene helps as a selection marker.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347272
Recombinant colonies in insertional inactivation are differentiated on the basis of
1 Production of blue coloured colonies
2 Production of no coloured (transparent) colonies
3 Production of white coloured colonies
4 Production of orange coloured colonies
Explanation:
The presence of a chromogenic substrate gives blue coloured colonies. if the plasmid in bacteria does not have an insert. The presence of insert results in the inactivation of enzyme \(\beta\)-galactosidase due to which colourless recombinant colonies are produced.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347273
Identify the enzyme (1) and (2) in the given diagram representing recombinant DNA technology.
347274
Which of the following are used as vector in gene cloning?
1 Plasmids
2 Mesosomes
3 Nucleoids
4 Lomasomes
Explanation:
Most commonly used cloning vectors are Plasmids that include three functional regions i) an origin of replication, ii) drug resistance gene iii) region where DNA can be inserted without interfering with plasmid replication (cloning sites).
347271
An antibiotic resistance gene in a vector usually helps in the selection of:
1 Competent bacterial cells
2 Transformed bacterial cells
3 Recombinant bacterial cells
4 None of the above
Explanation:
As a result only bacteria containing a plasmid with antibiotic resistance will grow in the presence of antibiotics. This gene helps as a selection marker.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347272
Recombinant colonies in insertional inactivation are differentiated on the basis of
1 Production of blue coloured colonies
2 Production of no coloured (transparent) colonies
3 Production of white coloured colonies
4 Production of orange coloured colonies
Explanation:
The presence of a chromogenic substrate gives blue coloured colonies. if the plasmid in bacteria does not have an insert. The presence of insert results in the inactivation of enzyme \(\beta\)-galactosidase due to which colourless recombinant colonies are produced.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347273
Identify the enzyme (1) and (2) in the given diagram representing recombinant DNA technology.
347274
Which of the following are used as vector in gene cloning?
1 Plasmids
2 Mesosomes
3 Nucleoids
4 Lomasomes
Explanation:
Most commonly used cloning vectors are Plasmids that include three functional regions i) an origin of replication, ii) drug resistance gene iii) region where DNA can be inserted without interfering with plasmid replication (cloning sites).
347271
An antibiotic resistance gene in a vector usually helps in the selection of:
1 Competent bacterial cells
2 Transformed bacterial cells
3 Recombinant bacterial cells
4 None of the above
Explanation:
As a result only bacteria containing a plasmid with antibiotic resistance will grow in the presence of antibiotics. This gene helps as a selection marker.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347272
Recombinant colonies in insertional inactivation are differentiated on the basis of
1 Production of blue coloured colonies
2 Production of no coloured (transparent) colonies
3 Production of white coloured colonies
4 Production of orange coloured colonies
Explanation:
The presence of a chromogenic substrate gives blue coloured colonies. if the plasmid in bacteria does not have an insert. The presence of insert results in the inactivation of enzyme \(\beta\)-galactosidase due to which colourless recombinant colonies are produced.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347273
Identify the enzyme (1) and (2) in the given diagram representing recombinant DNA technology.
347274
Which of the following are used as vector in gene cloning?
1 Plasmids
2 Mesosomes
3 Nucleoids
4 Lomasomes
Explanation:
Most commonly used cloning vectors are Plasmids that include three functional regions i) an origin of replication, ii) drug resistance gene iii) region where DNA can be inserted without interfering with plasmid replication (cloning sites).
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BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347271
An antibiotic resistance gene in a vector usually helps in the selection of:
1 Competent bacterial cells
2 Transformed bacterial cells
3 Recombinant bacterial cells
4 None of the above
Explanation:
As a result only bacteria containing a plasmid with antibiotic resistance will grow in the presence of antibiotics. This gene helps as a selection marker.
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347272
Recombinant colonies in insertional inactivation are differentiated on the basis of
1 Production of blue coloured colonies
2 Production of no coloured (transparent) colonies
3 Production of white coloured colonies
4 Production of orange coloured colonies
Explanation:
The presence of a chromogenic substrate gives blue coloured colonies. if the plasmid in bacteria does not have an insert. The presence of insert results in the inactivation of enzyme \(\beta\)-galactosidase due to which colourless recombinant colonies are produced.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347273
Identify the enzyme (1) and (2) in the given diagram representing recombinant DNA technology.
347274
Which of the following are used as vector in gene cloning?
1 Plasmids
2 Mesosomes
3 Nucleoids
4 Lomasomes
Explanation:
Most commonly used cloning vectors are Plasmids that include three functional regions i) an origin of replication, ii) drug resistance gene iii) region where DNA can be inserted without interfering with plasmid replication (cloning sites).
