1 Transfer of DNA fragments from in vitro cellulose membrane to electrophoresis gel
2 Attachment of probes to DNA fragments
3 Transfer of DNA fragments from electrophoresis gel to nitrocellulose sheet
4 Comparison of DNA fragments from two sources
Explanation:
A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis separated DNA fragments to a filter nitrocellulose membrane and subsequent fragment detection by probe hybridization.
