1 Construction of recombinant DNA by joining with cloning vectors
2 Cutting of DNA into fragments
3 Separation of DNA fragments according to their size
4 Isolation of DNA molecule
Explanation:
Gel electrophoresis is a technique generally used in laboratories to separate charged molecules such as DNA, RNA, and proteins according to their size.
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342668
DNA Fingerprinting involves the following steps, find out the missing steps i. A ii. Digestion of DNA by restriction endonucleases, iii. Separation of DNA fragments by electrophoresis, iv. B v. Hybridisation using labelled VNTR probe, and vi. C
1 A. isolation of RNA, B. transferring (blotting) of separated RNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised RNA fragments by autoradiography
2 A. isolation of RNA, and converting into DNA B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
3 A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
4 A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised RNA fragments by autoradiography
Explanation:
A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342669
DNA fingerprinting works because-
1 There are mutiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual
2 DNA in the skin cells is very diverse
3 Genes containing the same alleles make it simple to compare different individuals
4 PCR allows amplification of proteins from single cells
Explanation:
DNA fingerprinting works because- there are multiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342670
In which of the following steps in DNA fingerprinting technique are labelled VNTR probes used?
1 During isolation of DNA
2 During digestion of DNA by REN
3 During electrophoresis
4 During hybridisation
Explanation:
The human genome contains a number of short DNA sequences that are present as tandem repeats of varied lengths at several chromosomal locations. These Variable Number of Tandem Repeats (VNTR) are important components of DNA fingerprints. When DNA is cut with restriction endonucleases, a pattern of bands is produced. It can be visualized by Southern blot hybridization using radio labelled VNTR sequences as probes. This pattern is known as DNA fingerprints.
1 Construction of recombinant DNA by joining with cloning vectors
2 Cutting of DNA into fragments
3 Separation of DNA fragments according to their size
4 Isolation of DNA molecule
Explanation:
Gel electrophoresis is a technique generally used in laboratories to separate charged molecules such as DNA, RNA, and proteins according to their size.
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342668
DNA Fingerprinting involves the following steps, find out the missing steps i. A ii. Digestion of DNA by restriction endonucleases, iii. Separation of DNA fragments by electrophoresis, iv. B v. Hybridisation using labelled VNTR probe, and vi. C
1 A. isolation of RNA, B. transferring (blotting) of separated RNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised RNA fragments by autoradiography
2 A. isolation of RNA, and converting into DNA B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
3 A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
4 A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised RNA fragments by autoradiography
Explanation:
A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342669
DNA fingerprinting works because-
1 There are mutiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual
2 DNA in the skin cells is very diverse
3 Genes containing the same alleles make it simple to compare different individuals
4 PCR allows amplification of proteins from single cells
Explanation:
DNA fingerprinting works because- there are multiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342670
In which of the following steps in DNA fingerprinting technique are labelled VNTR probes used?
1 During isolation of DNA
2 During digestion of DNA by REN
3 During electrophoresis
4 During hybridisation
Explanation:
The human genome contains a number of short DNA sequences that are present as tandem repeats of varied lengths at several chromosomal locations. These Variable Number of Tandem Repeats (VNTR) are important components of DNA fingerprints. When DNA is cut with restriction endonucleases, a pattern of bands is produced. It can be visualized by Southern blot hybridization using radio labelled VNTR sequences as probes. This pattern is known as DNA fingerprints.
1 Construction of recombinant DNA by joining with cloning vectors
2 Cutting of DNA into fragments
3 Separation of DNA fragments according to their size
4 Isolation of DNA molecule
Explanation:
Gel electrophoresis is a technique generally used in laboratories to separate charged molecules such as DNA, RNA, and proteins according to their size.
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342668
DNA Fingerprinting involves the following steps, find out the missing steps i. A ii. Digestion of DNA by restriction endonucleases, iii. Separation of DNA fragments by electrophoresis, iv. B v. Hybridisation using labelled VNTR probe, and vi. C
1 A. isolation of RNA, B. transferring (blotting) of separated RNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised RNA fragments by autoradiography
2 A. isolation of RNA, and converting into DNA B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
3 A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
4 A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised RNA fragments by autoradiography
Explanation:
A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342669
DNA fingerprinting works because-
1 There are mutiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual
2 DNA in the skin cells is very diverse
3 Genes containing the same alleles make it simple to compare different individuals
4 PCR allows amplification of proteins from single cells
Explanation:
DNA fingerprinting works because- there are multiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342670
In which of the following steps in DNA fingerprinting technique are labelled VNTR probes used?
1 During isolation of DNA
2 During digestion of DNA by REN
3 During electrophoresis
4 During hybridisation
Explanation:
The human genome contains a number of short DNA sequences that are present as tandem repeats of varied lengths at several chromosomal locations. These Variable Number of Tandem Repeats (VNTR) are important components of DNA fingerprints. When DNA is cut with restriction endonucleases, a pattern of bands is produced. It can be visualized by Southern blot hybridization using radio labelled VNTR sequences as probes. This pattern is known as DNA fingerprints.
1 Construction of recombinant DNA by joining with cloning vectors
2 Cutting of DNA into fragments
3 Separation of DNA fragments according to their size
4 Isolation of DNA molecule
Explanation:
Gel electrophoresis is a technique generally used in laboratories to separate charged molecules such as DNA, RNA, and proteins according to their size.
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342668
DNA Fingerprinting involves the following steps, find out the missing steps i. A ii. Digestion of DNA by restriction endonucleases, iii. Separation of DNA fragments by electrophoresis, iv. B v. Hybridisation using labelled VNTR probe, and vi. C
1 A. isolation of RNA, B. transferring (blotting) of separated RNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised RNA fragments by autoradiography
2 A. isolation of RNA, and converting into DNA B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
3 A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
4 A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised RNA fragments by autoradiography
Explanation:
A. isolation of DNA, B. transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon C. detection of hybridised DNA fragments by autoradiography
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342669
DNA fingerprinting works because-
1 There are mutiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual
2 DNA in the skin cells is very diverse
3 Genes containing the same alleles make it simple to compare different individuals
4 PCR allows amplification of proteins from single cells
Explanation:
DNA fingerprinting works because- there are multiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual
BIOXII06: MOLECULAR BASIS OF INHERITANCE
342670
In which of the following steps in DNA fingerprinting technique are labelled VNTR probes used?
1 During isolation of DNA
2 During digestion of DNA by REN
3 During electrophoresis
4 During hybridisation
Explanation:
The human genome contains a number of short DNA sequences that are present as tandem repeats of varied lengths at several chromosomal locations. These Variable Number of Tandem Repeats (VNTR) are important components of DNA fingerprints. When DNA is cut with restriction endonucleases, a pattern of bands is produced. It can be visualized by Southern blot hybridization using radio labelled VNTR sequences as probes. This pattern is known as DNA fingerprints.
