NEET Test Series from KOTA - 10 Papers In MS WORD
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BIOXII06: MOLECULAR BASIS OF INHERITANCE
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The term, 'southern blotting' refers to:
1 Transfer of DNA fragments from in vitro cellulose membrane to electrophoresis gel
2 Attachment of probes to DNA fragments
3 Transfer of DNA fragments from electrophoresis gel to nitrocellulose sheet
4 Comparison of DNA fragments from two sources
Explanation:
A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis separated DNA fragments to a filter nitrocellulose membrane and subsequent fragment detection by probe hybridization.