01. Role of Biotechnological Applications in Medicines
Biotechnology and its Applications

170939 Some of the common steps involved in the production of humulin are given below. Choose the correct sequence.
(I) Synthesis of gene (DNA) for human insulin artificially.
(II) Culturing of recombinant E. coli in bacteria.
(III) Purification of humulin.
(IV) Insertion of human insulin gene in plasmid DNA.
(V) Introduction of recombinant plasmid into E. coli.
(VI) Extraction of recombinant gene product from E. coli.

1 II, I, IV, III, V, VI
2 I, III, V, VI, II, IV
3 I, IV, V, II, VI, III
4 III, V, II, I, VI, IV
Biotechnology and its Applications

170947 Plasmid pBR322 has Pst-I restriction enzyme site within gene $\mathrm{amp}^{\mathrm{R}}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain

1 It will be able to produce a novel protein with dual ability
2 It will not be able to confer ampicillin resistance to the host cell
3 The transformed cells will have the ability to resist ampicillin as well as produce $\beta$ galactoside
4 It will lead to lysis of host cell
Biotechnology and its Applications

170942 Kohler and Milstein developed biotechnology for:

1 monoclonal antibodies
2 steroid synthesis
3 immobilization of enzymes
4 myeloma
Biotechnology and its Applications

170959 Consider the following statements about therapeutic drugs. Which of the statements given below are correct?
(i) The recombinant DNA technology is used for production of therapeutic drugs which are safe and effective
(ii) It avoids unwanted immunological responses, commonly observed with similar products isolated from non-human sources
(iii) About thirty recombinant therapeutics have been approved for human use in the world including India
Options:

1 (i) \& (ii) only
2 (i) \& (iii) only
3 (ii) \& (iii) only
4 (i), (ii) \& (iii) only
Biotechnology and its Applications

170963 Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially
(ii) Culturing recombinant E.coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E. coli
(vi) Extraction of recombinant gene product from $E$. coli.

1 i, iii, v, vi, ii, iv
2 ii, i , iv, iii, v, vi
3 iii , v, ii, i, vi, iv
4 i, iv , v, ii, vi, iii
Biotechnology and its Applications

170939 Some of the common steps involved in the production of humulin are given below. Choose the correct sequence.
(I) Synthesis of gene (DNA) for human insulin artificially.
(II) Culturing of recombinant E. coli in bacteria.
(III) Purification of humulin.
(IV) Insertion of human insulin gene in plasmid DNA.
(V) Introduction of recombinant plasmid into E. coli.
(VI) Extraction of recombinant gene product from E. coli.

1 II, I, IV, III, V, VI
2 I, III, V, VI, II, IV
3 I, IV, V, II, VI, III
4 III, V, II, I, VI, IV
Biotechnology and its Applications

170947 Plasmid pBR322 has Pst-I restriction enzyme site within gene $\mathrm{amp}^{\mathrm{R}}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain

1 It will be able to produce a novel protein with dual ability
2 It will not be able to confer ampicillin resistance to the host cell
3 The transformed cells will have the ability to resist ampicillin as well as produce $\beta$ galactoside
4 It will lead to lysis of host cell
Biotechnology and its Applications

170942 Kohler and Milstein developed biotechnology for:

1 monoclonal antibodies
2 steroid synthesis
3 immobilization of enzymes
4 myeloma
Biotechnology and its Applications

170959 Consider the following statements about therapeutic drugs. Which of the statements given below are correct?
(i) The recombinant DNA technology is used for production of therapeutic drugs which are safe and effective
(ii) It avoids unwanted immunological responses, commonly observed with similar products isolated from non-human sources
(iii) About thirty recombinant therapeutics have been approved for human use in the world including India
Options:

1 (i) \& (ii) only
2 (i) \& (iii) only
3 (ii) \& (iii) only
4 (i), (ii) \& (iii) only
Biotechnology and its Applications

170963 Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially
(ii) Culturing recombinant E.coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E. coli
(vi) Extraction of recombinant gene product from $E$. coli.

1 i, iii, v, vi, ii, iv
2 ii, i , iv, iii, v, vi
3 iii , v, ii, i, vi, iv
4 i, iv , v, ii, vi, iii
Biotechnology and its Applications

170939 Some of the common steps involved in the production of humulin are given below. Choose the correct sequence.
(I) Synthesis of gene (DNA) for human insulin artificially.
(II) Culturing of recombinant E. coli in bacteria.
(III) Purification of humulin.
(IV) Insertion of human insulin gene in plasmid DNA.
(V) Introduction of recombinant plasmid into E. coli.
(VI) Extraction of recombinant gene product from E. coli.

1 II, I, IV, III, V, VI
2 I, III, V, VI, II, IV
3 I, IV, V, II, VI, III
4 III, V, II, I, VI, IV
Biotechnology and its Applications

170947 Plasmid pBR322 has Pst-I restriction enzyme site within gene $\mathrm{amp}^{\mathrm{R}}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain

1 It will be able to produce a novel protein with dual ability
2 It will not be able to confer ampicillin resistance to the host cell
3 The transformed cells will have the ability to resist ampicillin as well as produce $\beta$ galactoside
4 It will lead to lysis of host cell
Biotechnology and its Applications

170942 Kohler and Milstein developed biotechnology for:

1 monoclonal antibodies
2 steroid synthesis
3 immobilization of enzymes
4 myeloma
Biotechnology and its Applications

170959 Consider the following statements about therapeutic drugs. Which of the statements given below are correct?
(i) The recombinant DNA technology is used for production of therapeutic drugs which are safe and effective
(ii) It avoids unwanted immunological responses, commonly observed with similar products isolated from non-human sources
(iii) About thirty recombinant therapeutics have been approved for human use in the world including India
Options:

1 (i) \& (ii) only
2 (i) \& (iii) only
3 (ii) \& (iii) only
4 (i), (ii) \& (iii) only
Biotechnology and its Applications

170963 Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially
(ii) Culturing recombinant E.coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E. coli
(vi) Extraction of recombinant gene product from $E$. coli.

1 i, iii, v, vi, ii, iv
2 ii, i , iv, iii, v, vi
3 iii , v, ii, i, vi, iv
4 i, iv , v, ii, vi, iii
Biotechnology and its Applications

170939 Some of the common steps involved in the production of humulin are given below. Choose the correct sequence.
(I) Synthesis of gene (DNA) for human insulin artificially.
(II) Culturing of recombinant E. coli in bacteria.
(III) Purification of humulin.
(IV) Insertion of human insulin gene in plasmid DNA.
(V) Introduction of recombinant plasmid into E. coli.
(VI) Extraction of recombinant gene product from E. coli.

1 II, I, IV, III, V, VI
2 I, III, V, VI, II, IV
3 I, IV, V, II, VI, III
4 III, V, II, I, VI, IV
Biotechnology and its Applications

170947 Plasmid pBR322 has Pst-I restriction enzyme site within gene $\mathrm{amp}^{\mathrm{R}}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain

1 It will be able to produce a novel protein with dual ability
2 It will not be able to confer ampicillin resistance to the host cell
3 The transformed cells will have the ability to resist ampicillin as well as produce $\beta$ galactoside
4 It will lead to lysis of host cell
Biotechnology and its Applications

170942 Kohler and Milstein developed biotechnology for:

1 monoclonal antibodies
2 steroid synthesis
3 immobilization of enzymes
4 myeloma
Biotechnology and its Applications

170959 Consider the following statements about therapeutic drugs. Which of the statements given below are correct?
(i) The recombinant DNA technology is used for production of therapeutic drugs which are safe and effective
(ii) It avoids unwanted immunological responses, commonly observed with similar products isolated from non-human sources
(iii) About thirty recombinant therapeutics have been approved for human use in the world including India
Options:

1 (i) \& (ii) only
2 (i) \& (iii) only
3 (ii) \& (iii) only
4 (i), (ii) \& (iii) only
Biotechnology and its Applications

170963 Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially
(ii) Culturing recombinant E.coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E. coli
(vi) Extraction of recombinant gene product from $E$. coli.

1 i, iii, v, vi, ii, iv
2 ii, i , iv, iii, v, vi
3 iii , v, ii, i, vi, iv
4 i, iv , v, ii, vi, iii
Biotechnology and its Applications

170939 Some of the common steps involved in the production of humulin are given below. Choose the correct sequence.
(I) Synthesis of gene (DNA) for human insulin artificially.
(II) Culturing of recombinant E. coli in bacteria.
(III) Purification of humulin.
(IV) Insertion of human insulin gene in plasmid DNA.
(V) Introduction of recombinant plasmid into E. coli.
(VI) Extraction of recombinant gene product from E. coli.

1 II, I, IV, III, V, VI
2 I, III, V, VI, II, IV
3 I, IV, V, II, VI, III
4 III, V, II, I, VI, IV
Biotechnology and its Applications

170947 Plasmid pBR322 has Pst-I restriction enzyme site within gene $\mathrm{amp}^{\mathrm{R}}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain

1 It will be able to produce a novel protein with dual ability
2 It will not be able to confer ampicillin resistance to the host cell
3 The transformed cells will have the ability to resist ampicillin as well as produce $\beta$ galactoside
4 It will lead to lysis of host cell
Biotechnology and its Applications

170942 Kohler and Milstein developed biotechnology for:

1 monoclonal antibodies
2 steroid synthesis
3 immobilization of enzymes
4 myeloma
Biotechnology and its Applications

170959 Consider the following statements about therapeutic drugs. Which of the statements given below are correct?
(i) The recombinant DNA technology is used for production of therapeutic drugs which are safe and effective
(ii) It avoids unwanted immunological responses, commonly observed with similar products isolated from non-human sources
(iii) About thirty recombinant therapeutics have been approved for human use in the world including India
Options:

1 (i) \& (ii) only
2 (i) \& (iii) only
3 (ii) \& (iii) only
4 (i), (ii) \& (iii) only
Biotechnology and its Applications

170963 Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially
(ii) Culturing recombinant E.coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E. coli
(vi) Extraction of recombinant gene product from $E$. coli.

1 i, iii, v, vi, ii, iv
2 ii, i , iv, iii, v, vi
3 iii , v, ii, i, vi, iv
4 i, iv , v, ii, vi, iii