NEET Test Series from KOTA - 10 Papers In MS WORD
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Biotechnology : Principles and Processes
171050
The linking of antibiotic resistance gene with the plasmid vector became possible with
1 DNA ligase
2 endonucleases
3 DNA polymerase
4 exonucleases
Explanation:
Ligase is an enzyme that catalyses the bond formation between the fragments of DNA. This catalytic reaction will only take place at the expense of the breakdown of ATP or other nucleotide phosphate. - DNA ligase is used to seal the nicks that remains in recombinant DNA molecule. Intact DNA ligase join together the neighboring nucleotide flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecule is a vector, which is desired DNA fragments has been inserted to enable its cloning in an appropriate host.
JCECE-2018 / BHU PMT (Screening)-2010
Biotechnology : Principles and Processes
171065
First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is
1 Hind II
2 EcoR I
3 Adenosine deaminase
4 Thermostable DNA polymerase
Explanation:
Restriction endonucleases are enzymes that cleave the phophodiester bond present between consecutive nucleotides near to specific DNA sequences that they identify. - The first restriction endonuclease characterized was hind II. It was isolated from the bacterium, Haemophilus influenza. - It recognizes a specific sequence of six base pairs. Eco RI is also REN and is isolated from the bacterium Escherichia coli.
NEET-2020 Phase-II
Biotechnology : Principles and Processes
171051
Separation of proteins on the basis of their being polyelectrolytes can be brought about by
1 Centrifugation
2 Electrophoresis
3 Chromatography
4 Crystallography
Explanation:
Electrophoresis is a technique for separating molecules of a mixture under the influence of an applied electric field. Dissolved molecule is an electric field more or migrate at a speed determined by charge/mass ratio. Proteins usually passes a net positive or negative charge due to charged amino acids present in them. Centrifugation:- Centrifugation is the most popular technique used in biological sciences for separation of particles/microorganisms by centrifuge A centrifuge is a device for separation of microorganism from the suspended fluid using centrifugal force ( 9 -force). Chromatography:- A physical method of separation in which the components to be separated are, distributed between two phases, one of them is stationary phase while the other is mobile phase, moves in a definite direction.
CG PMT-2007
Biotechnology : Principles and Processes
171053
Which of the following is not a restriction endonuclease?
1 EcoRI
2 Hind-III
3 Pst-I
4 DNAse-I
Explanation:
Restriction endonucleases are the enzyme which cut a DNA molecule within certain specific site that have specific base sequence. Ex- Eco-RI, Bam II, Hind-III, Pst-I etc. DNAse-I is not a restriction endonuclease enzyme. - DNAse-I is a specific endonuclease facilitating $\mid$ chromatin breakdown during apoptosis. DNAse-I $\mid$ activity is important to prevent immune stimulation and reduced activity may result in an increased risk for production of anti-nucleosome antibodies, a hall mark of SLE (systemic lypus erythematosus)
171050
The linking of antibiotic resistance gene with the plasmid vector became possible with
1 DNA ligase
2 endonucleases
3 DNA polymerase
4 exonucleases
Explanation:
Ligase is an enzyme that catalyses the bond formation between the fragments of DNA. This catalytic reaction will only take place at the expense of the breakdown of ATP or other nucleotide phosphate. - DNA ligase is used to seal the nicks that remains in recombinant DNA molecule. Intact DNA ligase join together the neighboring nucleotide flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecule is a vector, which is desired DNA fragments has been inserted to enable its cloning in an appropriate host.
JCECE-2018 / BHU PMT (Screening)-2010
Biotechnology : Principles and Processes
171065
First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is
1 Hind II
2 EcoR I
3 Adenosine deaminase
4 Thermostable DNA polymerase
Explanation:
Restriction endonucleases are enzymes that cleave the phophodiester bond present between consecutive nucleotides near to specific DNA sequences that they identify. - The first restriction endonuclease characterized was hind II. It was isolated from the bacterium, Haemophilus influenza. - It recognizes a specific sequence of six base pairs. Eco RI is also REN and is isolated from the bacterium Escherichia coli.
NEET-2020 Phase-II
Biotechnology : Principles and Processes
171051
Separation of proteins on the basis of their being polyelectrolytes can be brought about by
1 Centrifugation
2 Electrophoresis
3 Chromatography
4 Crystallography
Explanation:
Electrophoresis is a technique for separating molecules of a mixture under the influence of an applied electric field. Dissolved molecule is an electric field more or migrate at a speed determined by charge/mass ratio. Proteins usually passes a net positive or negative charge due to charged amino acids present in them. Centrifugation:- Centrifugation is the most popular technique used in biological sciences for separation of particles/microorganisms by centrifuge A centrifuge is a device for separation of microorganism from the suspended fluid using centrifugal force ( 9 -force). Chromatography:- A physical method of separation in which the components to be separated are, distributed between two phases, one of them is stationary phase while the other is mobile phase, moves in a definite direction.
CG PMT-2007
Biotechnology : Principles and Processes
171053
Which of the following is not a restriction endonuclease?
1 EcoRI
2 Hind-III
3 Pst-I
4 DNAse-I
Explanation:
Restriction endonucleases are the enzyme which cut a DNA molecule within certain specific site that have specific base sequence. Ex- Eco-RI, Bam II, Hind-III, Pst-I etc. DNAse-I is not a restriction endonuclease enzyme. - DNAse-I is a specific endonuclease facilitating $\mid$ chromatin breakdown during apoptosis. DNAse-I $\mid$ activity is important to prevent immune stimulation and reduced activity may result in an increased risk for production of anti-nucleosome antibodies, a hall mark of SLE (systemic lypus erythematosus)
171050
The linking of antibiotic resistance gene with the plasmid vector became possible with
1 DNA ligase
2 endonucleases
3 DNA polymerase
4 exonucleases
Explanation:
Ligase is an enzyme that catalyses the bond formation between the fragments of DNA. This catalytic reaction will only take place at the expense of the breakdown of ATP or other nucleotide phosphate. - DNA ligase is used to seal the nicks that remains in recombinant DNA molecule. Intact DNA ligase join together the neighboring nucleotide flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecule is a vector, which is desired DNA fragments has been inserted to enable its cloning in an appropriate host.
JCECE-2018 / BHU PMT (Screening)-2010
Biotechnology : Principles and Processes
171065
First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is
1 Hind II
2 EcoR I
3 Adenosine deaminase
4 Thermostable DNA polymerase
Explanation:
Restriction endonucleases are enzymes that cleave the phophodiester bond present between consecutive nucleotides near to specific DNA sequences that they identify. - The first restriction endonuclease characterized was hind II. It was isolated from the bacterium, Haemophilus influenza. - It recognizes a specific sequence of six base pairs. Eco RI is also REN and is isolated from the bacterium Escherichia coli.
NEET-2020 Phase-II
Biotechnology : Principles and Processes
171051
Separation of proteins on the basis of their being polyelectrolytes can be brought about by
1 Centrifugation
2 Electrophoresis
3 Chromatography
4 Crystallography
Explanation:
Electrophoresis is a technique for separating molecules of a mixture under the influence of an applied electric field. Dissolved molecule is an electric field more or migrate at a speed determined by charge/mass ratio. Proteins usually passes a net positive or negative charge due to charged amino acids present in them. Centrifugation:- Centrifugation is the most popular technique used in biological sciences for separation of particles/microorganisms by centrifuge A centrifuge is a device for separation of microorganism from the suspended fluid using centrifugal force ( 9 -force). Chromatography:- A physical method of separation in which the components to be separated are, distributed between two phases, one of them is stationary phase while the other is mobile phase, moves in a definite direction.
CG PMT-2007
Biotechnology : Principles and Processes
171053
Which of the following is not a restriction endonuclease?
1 EcoRI
2 Hind-III
3 Pst-I
4 DNAse-I
Explanation:
Restriction endonucleases are the enzyme which cut a DNA molecule within certain specific site that have specific base sequence. Ex- Eco-RI, Bam II, Hind-III, Pst-I etc. DNAse-I is not a restriction endonuclease enzyme. - DNAse-I is a specific endonuclease facilitating $\mid$ chromatin breakdown during apoptosis. DNAse-I $\mid$ activity is important to prevent immune stimulation and reduced activity may result in an increased risk for production of anti-nucleosome antibodies, a hall mark of SLE (systemic lypus erythematosus)
171050
The linking of antibiotic resistance gene with the plasmid vector became possible with
1 DNA ligase
2 endonucleases
3 DNA polymerase
4 exonucleases
Explanation:
Ligase is an enzyme that catalyses the bond formation between the fragments of DNA. This catalytic reaction will only take place at the expense of the breakdown of ATP or other nucleotide phosphate. - DNA ligase is used to seal the nicks that remains in recombinant DNA molecule. Intact DNA ligase join together the neighboring nucleotide flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecule is a vector, which is desired DNA fragments has been inserted to enable its cloning in an appropriate host.
JCECE-2018 / BHU PMT (Screening)-2010
Biotechnology : Principles and Processes
171065
First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is
1 Hind II
2 EcoR I
3 Adenosine deaminase
4 Thermostable DNA polymerase
Explanation:
Restriction endonucleases are enzymes that cleave the phophodiester bond present between consecutive nucleotides near to specific DNA sequences that they identify. - The first restriction endonuclease characterized was hind II. It was isolated from the bacterium, Haemophilus influenza. - It recognizes a specific sequence of six base pairs. Eco RI is also REN and is isolated from the bacterium Escherichia coli.
NEET-2020 Phase-II
Biotechnology : Principles and Processes
171051
Separation of proteins on the basis of their being polyelectrolytes can be brought about by
1 Centrifugation
2 Electrophoresis
3 Chromatography
4 Crystallography
Explanation:
Electrophoresis is a technique for separating molecules of a mixture under the influence of an applied electric field. Dissolved molecule is an electric field more or migrate at a speed determined by charge/mass ratio. Proteins usually passes a net positive or negative charge due to charged amino acids present in them. Centrifugation:- Centrifugation is the most popular technique used in biological sciences for separation of particles/microorganisms by centrifuge A centrifuge is a device for separation of microorganism from the suspended fluid using centrifugal force ( 9 -force). Chromatography:- A physical method of separation in which the components to be separated are, distributed between two phases, one of them is stationary phase while the other is mobile phase, moves in a definite direction.
CG PMT-2007
Biotechnology : Principles and Processes
171053
Which of the following is not a restriction endonuclease?
1 EcoRI
2 Hind-III
3 Pst-I
4 DNAse-I
Explanation:
Restriction endonucleases are the enzyme which cut a DNA molecule within certain specific site that have specific base sequence. Ex- Eco-RI, Bam II, Hind-III, Pst-I etc. DNAse-I is not a restriction endonuclease enzyme. - DNAse-I is a specific endonuclease facilitating $\mid$ chromatin breakdown during apoptosis. DNAse-I $\mid$ activity is important to prevent immune stimulation and reduced activity may result in an increased risk for production of anti-nucleosome antibodies, a hall mark of SLE (systemic lypus erythematosus)
