NEET Test Series from KOTA - 10 Papers In MS WORD
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BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347608
Restriction endonuclease enzymes are used to cut ____
1 Single-stranded RNA at specific sites
2 Double-stranded DNA at specific sites
3 Single-stranded DNA at any random sites
4 Double-stranded DNA at any random sites
Explanation:
Restriction endonucleases enzymes cut double-stranded DNA molecules at specific sites called recognition sites which have specific base sequences.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347609
Which of the following tools of recombinant DNA technology is incorrectly paired with it's use ?
1 DNA polymerase - copies DNA sequence in the polymerase chain reaction
2 Reverse transcriptase - Used in translation
3 Restriction endonuclease-Production of DNA Fragment for gene cloning
4 DNA ligase - enzyme that joins the sticky ends
Explanation:
Reverse transcriptase is used in reverse translation, i.e. from m-RNA to DNA.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347610
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1 5'- CGTTCG - 3' 3' - ATCGTA - 5'
2 5' - GAATTC - 3' 3' - CTTAAG - 5'
3 5' - CACGTA - 3' 3' - CTCAGT - 5'
4 5'- GATATG - 3' 3' - GTACTA - 5'
Explanation:
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Restriction enzymes recognize a specific sequence of nucleotides and produce a double-stranded cut in the DNA. Many of them are palindromic, meaning the base sequence reads the same backwards and forwards. Recognition sequences in DNA differ for each restriction enzyme, producing differences in the length, sequence and strand orientation (5' end or the 3' end) of a stickyend of an enzyme restriction. If we cut the DNA with EcoR1, the DNA would be cut right in the middle. All the pieces would be the same size, which would be \(15 \mathrm{~kb}\) long. Hence 5' GAATTC 3'; 3' CTTAAG 5' palindrome sequence can be easily cut at about the middle by EcoR1 enzyme.
AIPMT - 2010
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347611
Restriction endonucleases are
1 Present in human cells for degradation of DNA when a cell dies.
2 Used in genetic engineering for ligating two molecules
3 Synthesized by bacteria as a part of their defense mechanism
4 Used to create self DNA
Explanation:
Restriction endonucleases are molecular scissors which are used to cleave the DNA segments in Recombinant DNA technology. They are used in Invitro DNA synthesis.
347608
Restriction endonuclease enzymes are used to cut ____
1 Single-stranded RNA at specific sites
2 Double-stranded DNA at specific sites
3 Single-stranded DNA at any random sites
4 Double-stranded DNA at any random sites
Explanation:
Restriction endonucleases enzymes cut double-stranded DNA molecules at specific sites called recognition sites which have specific base sequences.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347609
Which of the following tools of recombinant DNA technology is incorrectly paired with it's use ?
1 DNA polymerase - copies DNA sequence in the polymerase chain reaction
2 Reverse transcriptase - Used in translation
3 Restriction endonuclease-Production of DNA Fragment for gene cloning
4 DNA ligase - enzyme that joins the sticky ends
Explanation:
Reverse transcriptase is used in reverse translation, i.e. from m-RNA to DNA.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347610
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1 5'- CGTTCG - 3' 3' - ATCGTA - 5'
2 5' - GAATTC - 3' 3' - CTTAAG - 5'
3 5' - CACGTA - 3' 3' - CTCAGT - 5'
4 5'- GATATG - 3' 3' - GTACTA - 5'
Explanation:
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Restriction enzymes recognize a specific sequence of nucleotides and produce a double-stranded cut in the DNA. Many of them are palindromic, meaning the base sequence reads the same backwards and forwards. Recognition sequences in DNA differ for each restriction enzyme, producing differences in the length, sequence and strand orientation (5' end or the 3' end) of a stickyend of an enzyme restriction. If we cut the DNA with EcoR1, the DNA would be cut right in the middle. All the pieces would be the same size, which would be \(15 \mathrm{~kb}\) long. Hence 5' GAATTC 3'; 3' CTTAAG 5' palindrome sequence can be easily cut at about the middle by EcoR1 enzyme.
AIPMT - 2010
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347611
Restriction endonucleases are
1 Present in human cells for degradation of DNA when a cell dies.
2 Used in genetic engineering for ligating two molecules
3 Synthesized by bacteria as a part of their defense mechanism
4 Used to create self DNA
Explanation:
Restriction endonucleases are molecular scissors which are used to cleave the DNA segments in Recombinant DNA technology. They are used in Invitro DNA synthesis.
347608
Restriction endonuclease enzymes are used to cut ____
1 Single-stranded RNA at specific sites
2 Double-stranded DNA at specific sites
3 Single-stranded DNA at any random sites
4 Double-stranded DNA at any random sites
Explanation:
Restriction endonucleases enzymes cut double-stranded DNA molecules at specific sites called recognition sites which have specific base sequences.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347609
Which of the following tools of recombinant DNA technology is incorrectly paired with it's use ?
1 DNA polymerase - copies DNA sequence in the polymerase chain reaction
2 Reverse transcriptase - Used in translation
3 Restriction endonuclease-Production of DNA Fragment for gene cloning
4 DNA ligase - enzyme that joins the sticky ends
Explanation:
Reverse transcriptase is used in reverse translation, i.e. from m-RNA to DNA.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347610
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1 5'- CGTTCG - 3' 3' - ATCGTA - 5'
2 5' - GAATTC - 3' 3' - CTTAAG - 5'
3 5' - CACGTA - 3' 3' - CTCAGT - 5'
4 5'- GATATG - 3' 3' - GTACTA - 5'
Explanation:
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Restriction enzymes recognize a specific sequence of nucleotides and produce a double-stranded cut in the DNA. Many of them are palindromic, meaning the base sequence reads the same backwards and forwards. Recognition sequences in DNA differ for each restriction enzyme, producing differences in the length, sequence and strand orientation (5' end or the 3' end) of a stickyend of an enzyme restriction. If we cut the DNA with EcoR1, the DNA would be cut right in the middle. All the pieces would be the same size, which would be \(15 \mathrm{~kb}\) long. Hence 5' GAATTC 3'; 3' CTTAAG 5' palindrome sequence can be easily cut at about the middle by EcoR1 enzyme.
AIPMT - 2010
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347611
Restriction endonucleases are
1 Present in human cells for degradation of DNA when a cell dies.
2 Used in genetic engineering for ligating two molecules
3 Synthesized by bacteria as a part of their defense mechanism
4 Used to create self DNA
Explanation:
Restriction endonucleases are molecular scissors which are used to cleave the DNA segments in Recombinant DNA technology. They are used in Invitro DNA synthesis.
347608
Restriction endonuclease enzymes are used to cut ____
1 Single-stranded RNA at specific sites
2 Double-stranded DNA at specific sites
3 Single-stranded DNA at any random sites
4 Double-stranded DNA at any random sites
Explanation:
Restriction endonucleases enzymes cut double-stranded DNA molecules at specific sites called recognition sites which have specific base sequences.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347609
Which of the following tools of recombinant DNA technology is incorrectly paired with it's use ?
1 DNA polymerase - copies DNA sequence in the polymerase chain reaction
2 Reverse transcriptase - Used in translation
3 Restriction endonuclease-Production of DNA Fragment for gene cloning
4 DNA ligase - enzyme that joins the sticky ends
Explanation:
Reverse transcriptase is used in reverse translation, i.e. from m-RNA to DNA.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347610
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1 5'- CGTTCG - 3' 3' - ATCGTA - 5'
2 5' - GAATTC - 3' 3' - CTTAAG - 5'
3 5' - CACGTA - 3' 3' - CTCAGT - 5'
4 5'- GATATG - 3' 3' - GTACTA - 5'
Explanation:
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Restriction enzymes recognize a specific sequence of nucleotides and produce a double-stranded cut in the DNA. Many of them are palindromic, meaning the base sequence reads the same backwards and forwards. Recognition sequences in DNA differ for each restriction enzyme, producing differences in the length, sequence and strand orientation (5' end or the 3' end) of a stickyend of an enzyme restriction. If we cut the DNA with EcoR1, the DNA would be cut right in the middle. All the pieces would be the same size, which would be \(15 \mathrm{~kb}\) long. Hence 5' GAATTC 3'; 3' CTTAAG 5' palindrome sequence can be easily cut at about the middle by EcoR1 enzyme.
AIPMT - 2010
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347611
Restriction endonucleases are
1 Present in human cells for degradation of DNA when a cell dies.
2 Used in genetic engineering for ligating two molecules
3 Synthesized by bacteria as a part of their defense mechanism
4 Used to create self DNA
Explanation:
Restriction endonucleases are molecular scissors which are used to cleave the DNA segments in Recombinant DNA technology. They are used in Invitro DNA synthesis.
