347436 Following are the steps involved in recombinant DNA technology, arrange them in the correct order
i. Transferring the recombinant DNA into the host
ii. Isolation of DNA,
iii. Ligation of the DNA fragment into a vector,
iv. Fragmentation of DNA by restriction endonucleases,
v. Isolation of a desired DNA fragment,
vi. Extraction of the desired product
vii. Culturing the host cells in a medium at large scale

347437 Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.
(A) Synthesis of gene (DNA) for human insulin artificially.
(B) Culturing recombinant
E. coli
in bioreactors.
(C) Purification of humulin
(D) Insertion of human insulin gene into plasmid.
(E) Introduction of recombinant plasmid into
E. coli
.
(F) Extraction of recombinant gene product from
E. coli
.

347438 A transformed bacterium with human gene, fails to produce desired protein. The reason could be:

347436 Following are the steps involved in recombinant DNA technology, arrange them in the correct order
i. Transferring the recombinant DNA into the host
ii. Isolation of DNA,
iii. Ligation of the DNA fragment into a vector,
iv. Fragmentation of DNA by restriction endonucleases,
v. Isolation of a desired DNA fragment,
vi. Extraction of the desired product
vii. Culturing the host cells in a medium at large scale

347437 Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.
(A) Synthesis of gene (DNA) for human insulin artificially.
(B) Culturing recombinant
E. coli
in bioreactors.
(C) Purification of humulin
(D) Insertion of human insulin gene into plasmid.
(E) Introduction of recombinant plasmid into
E. coli
.
(F) Extraction of recombinant gene product from
E. coli
.

347438 A transformed bacterium with human gene, fails to produce desired protein. The reason could be:

347436 Following are the steps involved in recombinant DNA technology, arrange them in the correct order
i. Transferring the recombinant DNA into the host
ii. Isolation of DNA,
iii. Ligation of the DNA fragment into a vector,
iv. Fragmentation of DNA by restriction endonucleases,
v. Isolation of a desired DNA fragment,
vi. Extraction of the desired product
vii. Culturing the host cells in a medium at large scale

347437 Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.
(A) Synthesis of gene (DNA) for human insulin artificially.
(B) Culturing recombinant
E. coli
in bioreactors.
(C) Purification of humulin
(D) Insertion of human insulin gene into plasmid.
(E) Introduction of recombinant plasmid into
E. coli
.
(F) Extraction of recombinant gene product from
E. coli
.

347438 A transformed bacterium with human gene, fails to produce desired protein. The reason could be: