Isolation of Genetic Material
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347405 During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

1 RNA
2 DNA
3 Histones
4 Polysaccharides
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347406 Which of the following statement is incorrect?

1 Nucleic acid is fragmented by nucleases
2 Construction of recombinant DNA involves cleaving DNA segments with endonuclease and rejoining with ligase.
3 Genetic engineering is making artificial limbs and diagnostic instruments
4 Ti plasmid transforms cells of plants
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347407 Which statement is correct?
I. Genes encoding resistance to antibiotics like ampicillin, chloramphenicol, tetracycline or kanamycin, are useful selectable markers for
E.coli.
II. Selectable markers helps to select the host cells which contain the vector and eliminate the non-transformants.
III. Large amount of recombinant protein can be produced by gene cloning.
IV. In the process of recombinant DNA technology, after several treatment the purified DNA is precipitated by adding chilled acetone.

1 I, II and III
2 Only IV
3 II, III and IV
4 All of these
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347408 Which enzymes are used to break the cell to release DNA

1 Lysozyme
2 Cellulase
3 Chitinase
4 All the above
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BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347405 During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

1 RNA
2 DNA
3 Histones
4 Polysaccharides
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347406 Which of the following statement is incorrect?

1 Nucleic acid is fragmented by nucleases
2 Construction of recombinant DNA involves cleaving DNA segments with endonuclease and rejoining with ligase.
3 Genetic engineering is making artificial limbs and diagnostic instruments
4 Ti plasmid transforms cells of plants
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347407 Which statement is correct?
I. Genes encoding resistance to antibiotics like ampicillin, chloramphenicol, tetracycline or kanamycin, are useful selectable markers for
E.coli.
II. Selectable markers helps to select the host cells which contain the vector and eliminate the non-transformants.
III. Large amount of recombinant protein can be produced by gene cloning.
IV. In the process of recombinant DNA technology, after several treatment the purified DNA is precipitated by adding chilled acetone.

1 I, II and III
2 Only IV
3 II, III and IV
4 All of these
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347408 Which enzymes are used to break the cell to release DNA

1 Lysozyme
2 Cellulase
3 Chitinase
4 All the above
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347405 During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

1 RNA
2 DNA
3 Histones
4 Polysaccharides
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347406 Which of the following statement is incorrect?

1 Nucleic acid is fragmented by nucleases
2 Construction of recombinant DNA involves cleaving DNA segments with endonuclease and rejoining with ligase.
3 Genetic engineering is making artificial limbs and diagnostic instruments
4 Ti plasmid transforms cells of plants
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347407 Which statement is correct?
I. Genes encoding resistance to antibiotics like ampicillin, chloramphenicol, tetracycline or kanamycin, are useful selectable markers for
E.coli.
II. Selectable markers helps to select the host cells which contain the vector and eliminate the non-transformants.
III. Large amount of recombinant protein can be produced by gene cloning.
IV. In the process of recombinant DNA technology, after several treatment the purified DNA is precipitated by adding chilled acetone.

1 I, II and III
2 Only IV
3 II, III and IV
4 All of these
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347408 Which enzymes are used to break the cell to release DNA

1 Lysozyme
2 Cellulase
3 Chitinase
4 All the above
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347405 During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

1 RNA
2 DNA
3 Histones
4 Polysaccharides
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347406 Which of the following statement is incorrect?

1 Nucleic acid is fragmented by nucleases
2 Construction of recombinant DNA involves cleaving DNA segments with endonuclease and rejoining with ligase.
3 Genetic engineering is making artificial limbs and diagnostic instruments
4 Ti plasmid transforms cells of plants
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347407 Which statement is correct?
I. Genes encoding resistance to antibiotics like ampicillin, chloramphenicol, tetracycline or kanamycin, are useful selectable markers for
E.coli.
II. Selectable markers helps to select the host cells which contain the vector and eliminate the non-transformants.
III. Large amount of recombinant protein can be produced by gene cloning.
IV. In the process of recombinant DNA technology, after several treatment the purified DNA is precipitated by adding chilled acetone.

1 I, II and III
2 Only IV
3 II, III and IV
4 All of these
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES

347408 Which enzymes are used to break the cell to release DNA

1 Lysozyme
2 Cellulase
3 Chitinase
4 All the above