2 Retroviruses convert common cells to cancerous cells
3 pBR 322 is a natural plasmid
4 Agrobacterium tumefaciens is a natural genetic engineer
Explanation:
T-DNA is present in \(A\). tumefaciens
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347361
Eukaryotic genes may not function properly when cloned into bacteria because of
1 Destruction by native endonucleases.
2 Inability to excise introns.
3 Failure of promoter to be recognized by bacterial RNA polymerase.
4 All of the above.
Explanation:
Eukaryotic genes may not function properly when cloned into bacteria because of (1) Destruction by native endonucleases. (2) Inability to excise introns. (3) Failure of promoter to be recognized by bacterial RNA polymerase.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347362
Statement A : In chemical mediated gene transfer method, certain chemicals such as \(\mathrm{Ca}\) phosphate help foreign DNA to enter the host cell Statement B : Microinjection method is used inplant cells.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
Micro-injection is a method used to Inject a recombinant DNA into the nucleus of an animal cell. This is the only way to introduce alien DNA into host cells. \(\mathrm{B}\) is incorrect statement.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347363
Significance of 'heat shock' method in bacterial transformation is to facilitate:
1 Binding of DNA to the cell wall
2 Uptake of DNA through membrane transport proteins
3 Uptake of DNA through transient pores in the bacterial cell wall
4 Expression of antibiotic resistance gene
Explanation:
Heat shock transformation alters membrane fluidity creating pores, in the plasma membrane of bacteria and allows for plasmid DNA to enter the bacterial cell
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347364
Biolistics (gene gun) is suitable for
1 DNA fingerprinting
2 Disarming pathogen vectors
3 Constructing recombinant DNA by joining with vectors
4 Transformation of plant cells
Explanation:
Biolistics (gene gun) is suitable for transformation of plant cells.
2 Retroviruses convert common cells to cancerous cells
3 pBR 322 is a natural plasmid
4 Agrobacterium tumefaciens is a natural genetic engineer
Explanation:
T-DNA is present in \(A\). tumefaciens
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347361
Eukaryotic genes may not function properly when cloned into bacteria because of
1 Destruction by native endonucleases.
2 Inability to excise introns.
3 Failure of promoter to be recognized by bacterial RNA polymerase.
4 All of the above.
Explanation:
Eukaryotic genes may not function properly when cloned into bacteria because of (1) Destruction by native endonucleases. (2) Inability to excise introns. (3) Failure of promoter to be recognized by bacterial RNA polymerase.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347362
Statement A : In chemical mediated gene transfer method, certain chemicals such as \(\mathrm{Ca}\) phosphate help foreign DNA to enter the host cell Statement B : Microinjection method is used inplant cells.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
Micro-injection is a method used to Inject a recombinant DNA into the nucleus of an animal cell. This is the only way to introduce alien DNA into host cells. \(\mathrm{B}\) is incorrect statement.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347363
Significance of 'heat shock' method in bacterial transformation is to facilitate:
1 Binding of DNA to the cell wall
2 Uptake of DNA through membrane transport proteins
3 Uptake of DNA through transient pores in the bacterial cell wall
4 Expression of antibiotic resistance gene
Explanation:
Heat shock transformation alters membrane fluidity creating pores, in the plasma membrane of bacteria and allows for plasmid DNA to enter the bacterial cell
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347364
Biolistics (gene gun) is suitable for
1 DNA fingerprinting
2 Disarming pathogen vectors
3 Constructing recombinant DNA by joining with vectors
4 Transformation of plant cells
Explanation:
Biolistics (gene gun) is suitable for transformation of plant cells.
2 Retroviruses convert common cells to cancerous cells
3 pBR 322 is a natural plasmid
4 Agrobacterium tumefaciens is a natural genetic engineer
Explanation:
T-DNA is present in \(A\). tumefaciens
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347361
Eukaryotic genes may not function properly when cloned into bacteria because of
1 Destruction by native endonucleases.
2 Inability to excise introns.
3 Failure of promoter to be recognized by bacterial RNA polymerase.
4 All of the above.
Explanation:
Eukaryotic genes may not function properly when cloned into bacteria because of (1) Destruction by native endonucleases. (2) Inability to excise introns. (3) Failure of promoter to be recognized by bacterial RNA polymerase.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347362
Statement A : In chemical mediated gene transfer method, certain chemicals such as \(\mathrm{Ca}\) phosphate help foreign DNA to enter the host cell Statement B : Microinjection method is used inplant cells.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
Micro-injection is a method used to Inject a recombinant DNA into the nucleus of an animal cell. This is the only way to introduce alien DNA into host cells. \(\mathrm{B}\) is incorrect statement.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347363
Significance of 'heat shock' method in bacterial transformation is to facilitate:
1 Binding of DNA to the cell wall
2 Uptake of DNA through membrane transport proteins
3 Uptake of DNA through transient pores in the bacterial cell wall
4 Expression of antibiotic resistance gene
Explanation:
Heat shock transformation alters membrane fluidity creating pores, in the plasma membrane of bacteria and allows for plasmid DNA to enter the bacterial cell
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347364
Biolistics (gene gun) is suitable for
1 DNA fingerprinting
2 Disarming pathogen vectors
3 Constructing recombinant DNA by joining with vectors
4 Transformation of plant cells
Explanation:
Biolistics (gene gun) is suitable for transformation of plant cells.
2 Retroviruses convert common cells to cancerous cells
3 pBR 322 is a natural plasmid
4 Agrobacterium tumefaciens is a natural genetic engineer
Explanation:
T-DNA is present in \(A\). tumefaciens
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347361
Eukaryotic genes may not function properly when cloned into bacteria because of
1 Destruction by native endonucleases.
2 Inability to excise introns.
3 Failure of promoter to be recognized by bacterial RNA polymerase.
4 All of the above.
Explanation:
Eukaryotic genes may not function properly when cloned into bacteria because of (1) Destruction by native endonucleases. (2) Inability to excise introns. (3) Failure of promoter to be recognized by bacterial RNA polymerase.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347362
Statement A : In chemical mediated gene transfer method, certain chemicals such as \(\mathrm{Ca}\) phosphate help foreign DNA to enter the host cell Statement B : Microinjection method is used inplant cells.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
Micro-injection is a method used to Inject a recombinant DNA into the nucleus of an animal cell. This is the only way to introduce alien DNA into host cells. \(\mathrm{B}\) is incorrect statement.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347363
Significance of 'heat shock' method in bacterial transformation is to facilitate:
1 Binding of DNA to the cell wall
2 Uptake of DNA through membrane transport proteins
3 Uptake of DNA through transient pores in the bacterial cell wall
4 Expression of antibiotic resistance gene
Explanation:
Heat shock transformation alters membrane fluidity creating pores, in the plasma membrane of bacteria and allows for plasmid DNA to enter the bacterial cell
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347364
Biolistics (gene gun) is suitable for
1 DNA fingerprinting
2 Disarming pathogen vectors
3 Constructing recombinant DNA by joining with vectors
4 Transformation of plant cells
Explanation:
Biolistics (gene gun) is suitable for transformation of plant cells.
2 Retroviruses convert common cells to cancerous cells
3 pBR 322 is a natural plasmid
4 Agrobacterium tumefaciens is a natural genetic engineer
Explanation:
T-DNA is present in \(A\). tumefaciens
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347361
Eukaryotic genes may not function properly when cloned into bacteria because of
1 Destruction by native endonucleases.
2 Inability to excise introns.
3 Failure of promoter to be recognized by bacterial RNA polymerase.
4 All of the above.
Explanation:
Eukaryotic genes may not function properly when cloned into bacteria because of (1) Destruction by native endonucleases. (2) Inability to excise introns. (3) Failure of promoter to be recognized by bacterial RNA polymerase.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347362
Statement A : In chemical mediated gene transfer method, certain chemicals such as \(\mathrm{Ca}\) phosphate help foreign DNA to enter the host cell Statement B : Microinjection method is used inplant cells.
1 Statement A is correct but Statement B is incorrect.
2 Statement A is incorrect but Statement B is correct.
3 Both statements are correct.
4 Both Statements are incorrect.
Explanation:
Micro-injection is a method used to Inject a recombinant DNA into the nucleus of an animal cell. This is the only way to introduce alien DNA into host cells. \(\mathrm{B}\) is incorrect statement.
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347363
Significance of 'heat shock' method in bacterial transformation is to facilitate:
1 Binding of DNA to the cell wall
2 Uptake of DNA through membrane transport proteins
3 Uptake of DNA through transient pores in the bacterial cell wall
4 Expression of antibiotic resistance gene
Explanation:
Heat shock transformation alters membrane fluidity creating pores, in the plasma membrane of bacteria and allows for plasmid DNA to enter the bacterial cell
NCERT Exemplar
BIOXII11:BIOTECHNOLOGY PRINCIPLES AND PROCESSES
347364
Biolistics (gene gun) is suitable for
1 DNA fingerprinting
2 Disarming pathogen vectors
3 Constructing recombinant DNA by joining with vectors
4 Transformation of plant cells
Explanation:
Biolistics (gene gun) is suitable for transformation of plant cells.
