171073
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
1 Retrovirus
2 Ti plasmid
3 $\mathrm{pBR} 322$
4 $\lambda$ phage
Explanation:
Retrovirus is a virus that uses RNA as its genetic material. Retrovirus commonly used as vector for introducing a DNA fragment in human lymphocyte and replace the functioning of a defected gene. - Ti plasmid-In Agrobacterium tumefaciens Ti plasmid is found which means tumour - inducing plasmid. - pBR322- It is a plasmid and was one of the first widely used E.coli cloning vector. - $\lambda$ - phage - It is a temperate bacteriophage that infects E.coli. It is a virus particle consisting of a head, containing 48514 base-pair of double- stranded linear DNA as its genetic material, and a tail that can have tail fibers.
NEET-2018
Biotechnology : Principles and Processes
171086
Arrange the following in sequential order of their usage in recombinant DNA technology. (i) Calcium chloride (ii) DNA ligase (iii) Ethylene diamine tetra acetic acid (iv) Restriction endonuclease The correct match is
1 (i), (iv), (iii) and (ii)
2 (iv), (i), (ii) and (iii)
3 (i), (iv), (ii) and (iii)
4 (iii), (iv), (ii) and (i)
Explanation:
Ethylene diamine tetra acetic acid (EDTA)- A chemical that binds certain metal ion such as Calcium, Magnesium, Lead and Iron. It is used in medicine to prevent blood samples from clotting and to remove calcium and lead from the body. It is also used to keep bacteria forming a boifilm (thin layer stuck to a surface). It is type of cheating agent. - Restriction endonuclease -Restriction endonucleases (REs) are bacterial enzymes that cleave double-stranded DNA. Type I REs are important in bacterial function but do not cleave DNA at specific sequences. These enzymes allow the cloning and purification of defined DNA fragments. - DNA ligase - DNA ligase is a specific type of enzyme, a ligase that facilitates the joining of DNA strands together by catalyzing the formation of a phasphodiester bond. It plays a role in repairing single stranded breaks in duplex DNA in living organisms but some forms may specifically repair double-strand breaks. - Calcium chloride- Calcium chloride is an inorganic compound, a salt with the chemical formula $\mathrm{CaCl}_2$. It is a white colored crystalline solid at room temperature, and it is highly soluble in water. It can be created by neutralizing hydrochloric and with calcium hydroxide. It is used for R-DNA into host cell.
AP EAMCET-2013
Biotechnology : Principles and Processes
171087
Assertion (A) : EcoRI makes staggered cut to produce sticky ends in a DNA double helix. Reason (R) : EcoRI cuts the DNA in between G and $A$ at the palindromic sequence $5^{\prime}$ GAATTC 3 3 . The correct option among the following is:
1 (A) is true, (R) is true and (R) is the correct explanation for (A)
2 (A) is true, (R) is true but (R) is not the correct explanation for (A)
3 (A) is true but (R) is false
4 (A) is false but (R) is true
Explanation:
EcoRI is a restriction endonuclease enzyme isolated from species E.coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites. - EcoRI cuts the DNA in between G and A at the palindromic sequence 5'GAATTC 3'. - Thus, Assertion (A) is true, Reason (R) true and (R) is the correct explanation for (A).
TS EAMCET-30.07.2022 Shift-II
Biotechnology : Principles and Processes
171097
Assertion (A) : Molecular farming is large scale production of biochemical's from plants Reason (R) : Transgenic plants are bioreactors for commercial production of antibodies. The correct option among the following is Options:
1 (A) is true, (R) is true and (R) is the correct explanation for (A)
2 (A) is true, (R) is true but (R) is not the correct explanation for (A)
3 (A) is true but (R) is false
4 (A) is false but (R) is true
Explanation:
Molecular farming is a biotechnological program that includes the genetic modification of agriculture products to produce proteins and chemicals for commercial and pharmaceuticals purpose. Molecular farming is large scale production of biochemical from plants because transgenic plants are bioreactors for commercial production of antibodies.
171073
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
1 Retrovirus
2 Ti plasmid
3 $\mathrm{pBR} 322$
4 $\lambda$ phage
Explanation:
Retrovirus is a virus that uses RNA as its genetic material. Retrovirus commonly used as vector for introducing a DNA fragment in human lymphocyte and replace the functioning of a defected gene. - Ti plasmid-In Agrobacterium tumefaciens Ti plasmid is found which means tumour - inducing plasmid. - pBR322- It is a plasmid and was one of the first widely used E.coli cloning vector. - $\lambda$ - phage - It is a temperate bacteriophage that infects E.coli. It is a virus particle consisting of a head, containing 48514 base-pair of double- stranded linear DNA as its genetic material, and a tail that can have tail fibers.
NEET-2018
Biotechnology : Principles and Processes
171086
Arrange the following in sequential order of their usage in recombinant DNA technology. (i) Calcium chloride (ii) DNA ligase (iii) Ethylene diamine tetra acetic acid (iv) Restriction endonuclease The correct match is
1 (i), (iv), (iii) and (ii)
2 (iv), (i), (ii) and (iii)
3 (i), (iv), (ii) and (iii)
4 (iii), (iv), (ii) and (i)
Explanation:
Ethylene diamine tetra acetic acid (EDTA)- A chemical that binds certain metal ion such as Calcium, Magnesium, Lead and Iron. It is used in medicine to prevent blood samples from clotting and to remove calcium and lead from the body. It is also used to keep bacteria forming a boifilm (thin layer stuck to a surface). It is type of cheating agent. - Restriction endonuclease -Restriction endonucleases (REs) are bacterial enzymes that cleave double-stranded DNA. Type I REs are important in bacterial function but do not cleave DNA at specific sequences. These enzymes allow the cloning and purification of defined DNA fragments. - DNA ligase - DNA ligase is a specific type of enzyme, a ligase that facilitates the joining of DNA strands together by catalyzing the formation of a phasphodiester bond. It plays a role in repairing single stranded breaks in duplex DNA in living organisms but some forms may specifically repair double-strand breaks. - Calcium chloride- Calcium chloride is an inorganic compound, a salt with the chemical formula $\mathrm{CaCl}_2$. It is a white colored crystalline solid at room temperature, and it is highly soluble in water. It can be created by neutralizing hydrochloric and with calcium hydroxide. It is used for R-DNA into host cell.
AP EAMCET-2013
Biotechnology : Principles and Processes
171087
Assertion (A) : EcoRI makes staggered cut to produce sticky ends in a DNA double helix. Reason (R) : EcoRI cuts the DNA in between G and $A$ at the palindromic sequence $5^{\prime}$ GAATTC 3 3 . The correct option among the following is:
1 (A) is true, (R) is true and (R) is the correct explanation for (A)
2 (A) is true, (R) is true but (R) is not the correct explanation for (A)
3 (A) is true but (R) is false
4 (A) is false but (R) is true
Explanation:
EcoRI is a restriction endonuclease enzyme isolated from species E.coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites. - EcoRI cuts the DNA in between G and A at the palindromic sequence 5'GAATTC 3'. - Thus, Assertion (A) is true, Reason (R) true and (R) is the correct explanation for (A).
TS EAMCET-30.07.2022 Shift-II
Biotechnology : Principles and Processes
171097
Assertion (A) : Molecular farming is large scale production of biochemical's from plants Reason (R) : Transgenic plants are bioreactors for commercial production of antibodies. The correct option among the following is Options:
1 (A) is true, (R) is true and (R) is the correct explanation for (A)
2 (A) is true, (R) is true but (R) is not the correct explanation for (A)
3 (A) is true but (R) is false
4 (A) is false but (R) is true
Explanation:
Molecular farming is a biotechnological program that includes the genetic modification of agriculture products to produce proteins and chemicals for commercial and pharmaceuticals purpose. Molecular farming is large scale production of biochemical from plants because transgenic plants are bioreactors for commercial production of antibodies.
171073
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
1 Retrovirus
2 Ti plasmid
3 $\mathrm{pBR} 322$
4 $\lambda$ phage
Explanation:
Retrovirus is a virus that uses RNA as its genetic material. Retrovirus commonly used as vector for introducing a DNA fragment in human lymphocyte and replace the functioning of a defected gene. - Ti plasmid-In Agrobacterium tumefaciens Ti plasmid is found which means tumour - inducing plasmid. - pBR322- It is a plasmid and was one of the first widely used E.coli cloning vector. - $\lambda$ - phage - It is a temperate bacteriophage that infects E.coli. It is a virus particle consisting of a head, containing 48514 base-pair of double- stranded linear DNA as its genetic material, and a tail that can have tail fibers.
NEET-2018
Biotechnology : Principles and Processes
171086
Arrange the following in sequential order of their usage in recombinant DNA technology. (i) Calcium chloride (ii) DNA ligase (iii) Ethylene diamine tetra acetic acid (iv) Restriction endonuclease The correct match is
1 (i), (iv), (iii) and (ii)
2 (iv), (i), (ii) and (iii)
3 (i), (iv), (ii) and (iii)
4 (iii), (iv), (ii) and (i)
Explanation:
Ethylene diamine tetra acetic acid (EDTA)- A chemical that binds certain metal ion such as Calcium, Magnesium, Lead and Iron. It is used in medicine to prevent blood samples from clotting and to remove calcium and lead from the body. It is also used to keep bacteria forming a boifilm (thin layer stuck to a surface). It is type of cheating agent. - Restriction endonuclease -Restriction endonucleases (REs) are bacterial enzymes that cleave double-stranded DNA. Type I REs are important in bacterial function but do not cleave DNA at specific sequences. These enzymes allow the cloning and purification of defined DNA fragments. - DNA ligase - DNA ligase is a specific type of enzyme, a ligase that facilitates the joining of DNA strands together by catalyzing the formation of a phasphodiester bond. It plays a role in repairing single stranded breaks in duplex DNA in living organisms but some forms may specifically repair double-strand breaks. - Calcium chloride- Calcium chloride is an inorganic compound, a salt with the chemical formula $\mathrm{CaCl}_2$. It is a white colored crystalline solid at room temperature, and it is highly soluble in water. It can be created by neutralizing hydrochloric and with calcium hydroxide. It is used for R-DNA into host cell.
AP EAMCET-2013
Biotechnology : Principles and Processes
171087
Assertion (A) : EcoRI makes staggered cut to produce sticky ends in a DNA double helix. Reason (R) : EcoRI cuts the DNA in between G and $A$ at the palindromic sequence $5^{\prime}$ GAATTC 3 3 . The correct option among the following is:
1 (A) is true, (R) is true and (R) is the correct explanation for (A)
2 (A) is true, (R) is true but (R) is not the correct explanation for (A)
3 (A) is true but (R) is false
4 (A) is false but (R) is true
Explanation:
EcoRI is a restriction endonuclease enzyme isolated from species E.coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites. - EcoRI cuts the DNA in between G and A at the palindromic sequence 5'GAATTC 3'. - Thus, Assertion (A) is true, Reason (R) true and (R) is the correct explanation for (A).
TS EAMCET-30.07.2022 Shift-II
Biotechnology : Principles and Processes
171097
Assertion (A) : Molecular farming is large scale production of biochemical's from plants Reason (R) : Transgenic plants are bioreactors for commercial production of antibodies. The correct option among the following is Options:
1 (A) is true, (R) is true and (R) is the correct explanation for (A)
2 (A) is true, (R) is true but (R) is not the correct explanation for (A)
3 (A) is true but (R) is false
4 (A) is false but (R) is true
Explanation:
Molecular farming is a biotechnological program that includes the genetic modification of agriculture products to produce proteins and chemicals for commercial and pharmaceuticals purpose. Molecular farming is large scale production of biochemical from plants because transgenic plants are bioreactors for commercial production of antibodies.
171073
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
1 Retrovirus
2 Ti plasmid
3 $\mathrm{pBR} 322$
4 $\lambda$ phage
Explanation:
Retrovirus is a virus that uses RNA as its genetic material. Retrovirus commonly used as vector for introducing a DNA fragment in human lymphocyte and replace the functioning of a defected gene. - Ti plasmid-In Agrobacterium tumefaciens Ti plasmid is found which means tumour - inducing plasmid. - pBR322- It is a plasmid and was one of the first widely used E.coli cloning vector. - $\lambda$ - phage - It is a temperate bacteriophage that infects E.coli. It is a virus particle consisting of a head, containing 48514 base-pair of double- stranded linear DNA as its genetic material, and a tail that can have tail fibers.
NEET-2018
Biotechnology : Principles and Processes
171086
Arrange the following in sequential order of their usage in recombinant DNA technology. (i) Calcium chloride (ii) DNA ligase (iii) Ethylene diamine tetra acetic acid (iv) Restriction endonuclease The correct match is
1 (i), (iv), (iii) and (ii)
2 (iv), (i), (ii) and (iii)
3 (i), (iv), (ii) and (iii)
4 (iii), (iv), (ii) and (i)
Explanation:
Ethylene diamine tetra acetic acid (EDTA)- A chemical that binds certain metal ion such as Calcium, Magnesium, Lead and Iron. It is used in medicine to prevent blood samples from clotting and to remove calcium and lead from the body. It is also used to keep bacteria forming a boifilm (thin layer stuck to a surface). It is type of cheating agent. - Restriction endonuclease -Restriction endonucleases (REs) are bacterial enzymes that cleave double-stranded DNA. Type I REs are important in bacterial function but do not cleave DNA at specific sequences. These enzymes allow the cloning and purification of defined DNA fragments. - DNA ligase - DNA ligase is a specific type of enzyme, a ligase that facilitates the joining of DNA strands together by catalyzing the formation of a phasphodiester bond. It plays a role in repairing single stranded breaks in duplex DNA in living organisms but some forms may specifically repair double-strand breaks. - Calcium chloride- Calcium chloride is an inorganic compound, a salt with the chemical formula $\mathrm{CaCl}_2$. It is a white colored crystalline solid at room temperature, and it is highly soluble in water. It can be created by neutralizing hydrochloric and with calcium hydroxide. It is used for R-DNA into host cell.
AP EAMCET-2013
Biotechnology : Principles and Processes
171087
Assertion (A) : EcoRI makes staggered cut to produce sticky ends in a DNA double helix. Reason (R) : EcoRI cuts the DNA in between G and $A$ at the palindromic sequence $5^{\prime}$ GAATTC 3 3 . The correct option among the following is:
1 (A) is true, (R) is true and (R) is the correct explanation for (A)
2 (A) is true, (R) is true but (R) is not the correct explanation for (A)
3 (A) is true but (R) is false
4 (A) is false but (R) is true
Explanation:
EcoRI is a restriction endonuclease enzyme isolated from species E.coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites. - EcoRI cuts the DNA in between G and A at the palindromic sequence 5'GAATTC 3'. - Thus, Assertion (A) is true, Reason (R) true and (R) is the correct explanation for (A).
TS EAMCET-30.07.2022 Shift-II
Biotechnology : Principles and Processes
171097
Assertion (A) : Molecular farming is large scale production of biochemical's from plants Reason (R) : Transgenic plants are bioreactors for commercial production of antibodies. The correct option among the following is Options:
1 (A) is true, (R) is true and (R) is the correct explanation for (A)
2 (A) is true, (R) is true but (R) is not the correct explanation for (A)
3 (A) is true but (R) is false
4 (A) is false but (R) is true
Explanation:
Molecular farming is a biotechnological program that includes the genetic modification of agriculture products to produce proteins and chemicals for commercial and pharmaceuticals purpose. Molecular farming is large scale production of biochemical from plants because transgenic plants are bioreactors for commercial production of antibodies.
