02. Tools of Recombinant DNA Technology
Biotechnology : Principles and Processes

171079 In ti-plasmid, which of the following is removed:

1 Auxin gene
2 Virulent gene
3 Cytokinin gene
4 Auxin \& cytokinin gene
Biotechnology : Principles and Processes

171082 EcoRI is

1 a restriction enzyme
2 a plasmid
3 used to join two DNA fragments
4 the abbreviation for bacterium Escherichia coli
Biotechnology : Principles and Processes

171083 Identify the desirable characteristics for a plasmid used in rDNA technology from the following:
A. Ability to multiply and express outside the host in a bioreactor
B. A highly active promoter
C. A site at which replication can be initiated
D. One or more identifiable marker genes
E. One or more unique restriction sites

1 A, C and E only
2 B, C and E only
3 A, C, D, and E only
4 B, C, D, and E only
Biotechnology : Principles and Processes

171084 The enzymes which are absolutely necessary for recombinant DNA technology are :

1 Restriction endonucleases and topoisomerases
2 Endonucleases and polymerases
3 Restriction endonucleases and Ligases
4 Peptidases and Ligases
Biotechnology : Principles and Processes

171079 In ti-plasmid, which of the following is removed:

1 Auxin gene
2 Virulent gene
3 Cytokinin gene
4 Auxin \& cytokinin gene
Biotechnology : Principles and Processes

171082 EcoRI is

1 a restriction enzyme
2 a plasmid
3 used to join two DNA fragments
4 the abbreviation for bacterium Escherichia coli
Biotechnology : Principles and Processes

171083 Identify the desirable characteristics for a plasmid used in rDNA technology from the following:
A. Ability to multiply and express outside the host in a bioreactor
B. A highly active promoter
C. A site at which replication can be initiated
D. One or more identifiable marker genes
E. One or more unique restriction sites

1 A, C and E only
2 B, C and E only
3 A, C, D, and E only
4 B, C, D, and E only
Biotechnology : Principles and Processes

171084 The enzymes which are absolutely necessary for recombinant DNA technology are :

1 Restriction endonucleases and topoisomerases
2 Endonucleases and polymerases
3 Restriction endonucleases and Ligases
4 Peptidases and Ligases
Biotechnology : Principles and Processes

171079 In ti-plasmid, which of the following is removed:

1 Auxin gene
2 Virulent gene
3 Cytokinin gene
4 Auxin \& cytokinin gene
Biotechnology : Principles and Processes

171082 EcoRI is

1 a restriction enzyme
2 a plasmid
3 used to join two DNA fragments
4 the abbreviation for bacterium Escherichia coli
Biotechnology : Principles and Processes

171083 Identify the desirable characteristics for a plasmid used in rDNA technology from the following:
A. Ability to multiply and express outside the host in a bioreactor
B. A highly active promoter
C. A site at which replication can be initiated
D. One or more identifiable marker genes
E. One or more unique restriction sites

1 A, C and E only
2 B, C and E only
3 A, C, D, and E only
4 B, C, D, and E only
Biotechnology : Principles and Processes

171084 The enzymes which are absolutely necessary for recombinant DNA technology are :

1 Restriction endonucleases and topoisomerases
2 Endonucleases and polymerases
3 Restriction endonucleases and Ligases
4 Peptidases and Ligases
Biotechnology : Principles and Processes

171079 In ti-plasmid, which of the following is removed:

1 Auxin gene
2 Virulent gene
3 Cytokinin gene
4 Auxin \& cytokinin gene
Biotechnology : Principles and Processes

171082 EcoRI is

1 a restriction enzyme
2 a plasmid
3 used to join two DNA fragments
4 the abbreviation for bacterium Escherichia coli
Biotechnology : Principles and Processes

171083 Identify the desirable characteristics for a plasmid used in rDNA technology from the following:
A. Ability to multiply and express outside the host in a bioreactor
B. A highly active promoter
C. A site at which replication can be initiated
D. One or more identifiable marker genes
E. One or more unique restriction sites

1 A, C and E only
2 B, C and E only
3 A, C, D, and E only
4 B, C, D, and E only
Biotechnology : Principles and Processes

171084 The enzymes which are absolutely necessary for recombinant DNA technology are :

1 Restriction endonucleases and topoisomerases
2 Endonucleases and polymerases
3 Restriction endonucleases and Ligases
4 Peptidases and Ligases