171091
The figure below is the diagrammatic representation of the E. Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)? E.coli
1 Hindi III, EcoRI-selectable markers
2 ampR, tetR-antibiotic resistance genes
3 ori-original restriction enzyme
4 rop-reduced osmotic pressure
Explanation:
In pBR 322, ori-represents site or origin of replication rop site code for proteins that take part in the replication of plasmid. - ampR and tetR are antibiotic resistance genes. - Hind III, EcoRI- recognition sites of restriction| endonuclease. - EcoRI is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragment at specific sites and also a part of the restriction modification system.
AIPMT (Screening)-2012
Biotechnology : Principles and Processes
171045
Which of the following pairs is correctly matched ?
1 Central dogma - Codon
2 Okazaki fragments - Splicing
3 RNA polymerase - RNA primer
4 Restriction enzymes - Genetic engineering
Explanation:
Restriction enzyme :- It is a protein (nuclease) that recognize a specific, short nucleotide sequence of DNA. (Known as-restriction site or target sequence or recognition sequence) and then cuts the DNA at that specific site. Restriction enzyme are found in bacteria (and other prokaryotes). Each restriction enzyme, recognizes just one or few restriction site. More than 400 restriction enzymes have been isolated from the bacteria that manufacture them. Central dogma is a theory that show genetic information one direction, from DNA, to RNA, RNA directly to protein Okazaki fragments is found in DNA replication. RNA polymers- synthesize RNA molecular from a template of DNA through a process called transcription.
Punjab MET-2003
Biotechnology : Principles and Processes
171066
Which of the following restriction enzymes produces blunt ends?
1 Sall
2 Eco RV
3 Xho
4 Hind III
Explanation:
Eco RV is a type II restriction endonuclease isolated from E. coli it recognize palindromic sequence and produce blunt ends. $$ \begin{aligned} & 5^{\prime} \mathrm{G}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{C} 3^{\prime} \\ & 3^{\prime} \mathrm{C}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{G} 5^{\prime} \\ & \downarrow \text { Eco RV } \\ & \left.
l
5^ --
3^ --
\right]\left[
l
-- 3^
-- 5
\right. \\ & \end{aligned} $$ Blunt ends
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171067
The most throughly studied of the known bacteria-plant interactions is the :-
1 Gall formation on certain Angiosperms by Agrobacterium
2 Nodulation of Sesbania stems by nitrogen fixing bacteria
3 Plant growth stimulation by phosphatesolubilising bacteria
4 Cyanobacterial symbiosis with some aquatic ferns
Explanation:
Agrobacterium tumefaciens is Rod Shaped, gram negative soil bacterium. - The Agrobacterium Crown Gall phenomenon is extensively used as natural genetic engineer in plant genetic engineering. - It also an example of plant microbe interaction competition commensalism, mutualism and parasitism relationship given by (Campbell 1995). - Agrobacterium has been extensively studied both as a pathogen and an important biotechnological tool. - The infection process involves the transfer of t-DNA and virulence protein into the plant cell.
AIPMT-2004
Biotechnology : Principles and Processes
171071
Match the following enzymes with their functions:
1 A-(ii), B-(iv), C-(i), D-(iii)
2 A-(iii), B-(i), C-(iv), D-(ii)
3 A-(iii), B-(iv), C-(i), D-(ii)
4 A-(iv), B-(iii), C-(i), D-(ii)
Explanation:
Restriction endonuclease - Cuts DNA at specific position. - Restriction endonucleases recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. - Restriction exonuclease -Removes nucleotides From the ends of DNA. Restriction exonucleases remove nucleotides from the terminal ends (either $5^{\prime}$ or 3') of DNA of one strand of duplex. - DNA ligase - Join the DNA fragments. The DNA ligase is functional in joining the Okazaki fragments that take shape on the lagging strand while the DNA replicates. It is able to join the two DNA fragments as a result of the formation of a phosphodiester bond between them with the help of a molecule of energy. - Taq polymerase - Extends primers on genomic DNA template Thermus aquaticus (Taqs) polymerase, exhibits transcriptase activity when a DNA oligomer is used as a template and that genetic information of oligomer can be amplified by PCR using DNA primers.
171091
The figure below is the diagrammatic representation of the E. Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)? E.coli
1 Hindi III, EcoRI-selectable markers
2 ampR, tetR-antibiotic resistance genes
3 ori-original restriction enzyme
4 rop-reduced osmotic pressure
Explanation:
In pBR 322, ori-represents site or origin of replication rop site code for proteins that take part in the replication of plasmid. - ampR and tetR are antibiotic resistance genes. - Hind III, EcoRI- recognition sites of restriction| endonuclease. - EcoRI is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragment at specific sites and also a part of the restriction modification system.
AIPMT (Screening)-2012
Biotechnology : Principles and Processes
171045
Which of the following pairs is correctly matched ?
1 Central dogma - Codon
2 Okazaki fragments - Splicing
3 RNA polymerase - RNA primer
4 Restriction enzymes - Genetic engineering
Explanation:
Restriction enzyme :- It is a protein (nuclease) that recognize a specific, short nucleotide sequence of DNA. (Known as-restriction site or target sequence or recognition sequence) and then cuts the DNA at that specific site. Restriction enzyme are found in bacteria (and other prokaryotes). Each restriction enzyme, recognizes just one or few restriction site. More than 400 restriction enzymes have been isolated from the bacteria that manufacture them. Central dogma is a theory that show genetic information one direction, from DNA, to RNA, RNA directly to protein Okazaki fragments is found in DNA replication. RNA polymers- synthesize RNA molecular from a template of DNA through a process called transcription.
Punjab MET-2003
Biotechnology : Principles and Processes
171066
Which of the following restriction enzymes produces blunt ends?
1 Sall
2 Eco RV
3 Xho
4 Hind III
Explanation:
Eco RV is a type II restriction endonuclease isolated from E. coli it recognize palindromic sequence and produce blunt ends. $$ \begin{aligned} & 5^{\prime} \mathrm{G}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{C} 3^{\prime} \\ & 3^{\prime} \mathrm{C}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{G} 5^{\prime} \\ & \downarrow \text { Eco RV } \\ & \left.
l
5^ --
3^ --
\right]\left[
l
-- 3^
-- 5
\right. \\ & \end{aligned} $$ Blunt ends
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171067
The most throughly studied of the known bacteria-plant interactions is the :-
1 Gall formation on certain Angiosperms by Agrobacterium
2 Nodulation of Sesbania stems by nitrogen fixing bacteria
3 Plant growth stimulation by phosphatesolubilising bacteria
4 Cyanobacterial symbiosis with some aquatic ferns
Explanation:
Agrobacterium tumefaciens is Rod Shaped, gram negative soil bacterium. - The Agrobacterium Crown Gall phenomenon is extensively used as natural genetic engineer in plant genetic engineering. - It also an example of plant microbe interaction competition commensalism, mutualism and parasitism relationship given by (Campbell 1995). - Agrobacterium has been extensively studied both as a pathogen and an important biotechnological tool. - The infection process involves the transfer of t-DNA and virulence protein into the plant cell.
AIPMT-2004
Biotechnology : Principles and Processes
171071
Match the following enzymes with their functions:
1 A-(ii), B-(iv), C-(i), D-(iii)
2 A-(iii), B-(i), C-(iv), D-(ii)
3 A-(iii), B-(iv), C-(i), D-(ii)
4 A-(iv), B-(iii), C-(i), D-(ii)
Explanation:
Restriction endonuclease - Cuts DNA at specific position. - Restriction endonucleases recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. - Restriction exonuclease -Removes nucleotides From the ends of DNA. Restriction exonucleases remove nucleotides from the terminal ends (either $5^{\prime}$ or 3') of DNA of one strand of duplex. - DNA ligase - Join the DNA fragments. The DNA ligase is functional in joining the Okazaki fragments that take shape on the lagging strand while the DNA replicates. It is able to join the two DNA fragments as a result of the formation of a phosphodiester bond between them with the help of a molecule of energy. - Taq polymerase - Extends primers on genomic DNA template Thermus aquaticus (Taqs) polymerase, exhibits transcriptase activity when a DNA oligomer is used as a template and that genetic information of oligomer can be amplified by PCR using DNA primers.
171091
The figure below is the diagrammatic representation of the E. Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)? E.coli
1 Hindi III, EcoRI-selectable markers
2 ampR, tetR-antibiotic resistance genes
3 ori-original restriction enzyme
4 rop-reduced osmotic pressure
Explanation:
In pBR 322, ori-represents site or origin of replication rop site code for proteins that take part in the replication of plasmid. - ampR and tetR are antibiotic resistance genes. - Hind III, EcoRI- recognition sites of restriction| endonuclease. - EcoRI is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragment at specific sites and also a part of the restriction modification system.
AIPMT (Screening)-2012
Biotechnology : Principles and Processes
171045
Which of the following pairs is correctly matched ?
1 Central dogma - Codon
2 Okazaki fragments - Splicing
3 RNA polymerase - RNA primer
4 Restriction enzymes - Genetic engineering
Explanation:
Restriction enzyme :- It is a protein (nuclease) that recognize a specific, short nucleotide sequence of DNA. (Known as-restriction site or target sequence or recognition sequence) and then cuts the DNA at that specific site. Restriction enzyme are found in bacteria (and other prokaryotes). Each restriction enzyme, recognizes just one or few restriction site. More than 400 restriction enzymes have been isolated from the bacteria that manufacture them. Central dogma is a theory that show genetic information one direction, from DNA, to RNA, RNA directly to protein Okazaki fragments is found in DNA replication. RNA polymers- synthesize RNA molecular from a template of DNA through a process called transcription.
Punjab MET-2003
Biotechnology : Principles and Processes
171066
Which of the following restriction enzymes produces blunt ends?
1 Sall
2 Eco RV
3 Xho
4 Hind III
Explanation:
Eco RV is a type II restriction endonuclease isolated from E. coli it recognize palindromic sequence and produce blunt ends. $$ \begin{aligned} & 5^{\prime} \mathrm{G}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{C} 3^{\prime} \\ & 3^{\prime} \mathrm{C}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{G} 5^{\prime} \\ & \downarrow \text { Eco RV } \\ & \left.
l
5^ --
3^ --
\right]\left[
l
-- 3^
-- 5
\right. \\ & \end{aligned} $$ Blunt ends
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171067
The most throughly studied of the known bacteria-plant interactions is the :-
1 Gall formation on certain Angiosperms by Agrobacterium
2 Nodulation of Sesbania stems by nitrogen fixing bacteria
3 Plant growth stimulation by phosphatesolubilising bacteria
4 Cyanobacterial symbiosis with some aquatic ferns
Explanation:
Agrobacterium tumefaciens is Rod Shaped, gram negative soil bacterium. - The Agrobacterium Crown Gall phenomenon is extensively used as natural genetic engineer in plant genetic engineering. - It also an example of plant microbe interaction competition commensalism, mutualism and parasitism relationship given by (Campbell 1995). - Agrobacterium has been extensively studied both as a pathogen and an important biotechnological tool. - The infection process involves the transfer of t-DNA and virulence protein into the plant cell.
AIPMT-2004
Biotechnology : Principles and Processes
171071
Match the following enzymes with their functions:
1 A-(ii), B-(iv), C-(i), D-(iii)
2 A-(iii), B-(i), C-(iv), D-(ii)
3 A-(iii), B-(iv), C-(i), D-(ii)
4 A-(iv), B-(iii), C-(i), D-(ii)
Explanation:
Restriction endonuclease - Cuts DNA at specific position. - Restriction endonucleases recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. - Restriction exonuclease -Removes nucleotides From the ends of DNA. Restriction exonucleases remove nucleotides from the terminal ends (either $5^{\prime}$ or 3') of DNA of one strand of duplex. - DNA ligase - Join the DNA fragments. The DNA ligase is functional in joining the Okazaki fragments that take shape on the lagging strand while the DNA replicates. It is able to join the two DNA fragments as a result of the formation of a phosphodiester bond between them with the help of a molecule of energy. - Taq polymerase - Extends primers on genomic DNA template Thermus aquaticus (Taqs) polymerase, exhibits transcriptase activity when a DNA oligomer is used as a template and that genetic information of oligomer can be amplified by PCR using DNA primers.
NEET Test Series from KOTA - 10 Papers In MS WORD
WhatsApp Here
Biotechnology : Principles and Processes
171091
The figure below is the diagrammatic representation of the E. Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)? E.coli
1 Hindi III, EcoRI-selectable markers
2 ampR, tetR-antibiotic resistance genes
3 ori-original restriction enzyme
4 rop-reduced osmotic pressure
Explanation:
In pBR 322, ori-represents site or origin of replication rop site code for proteins that take part in the replication of plasmid. - ampR and tetR are antibiotic resistance genes. - Hind III, EcoRI- recognition sites of restriction| endonuclease. - EcoRI is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragment at specific sites and also a part of the restriction modification system.
AIPMT (Screening)-2012
Biotechnology : Principles and Processes
171045
Which of the following pairs is correctly matched ?
1 Central dogma - Codon
2 Okazaki fragments - Splicing
3 RNA polymerase - RNA primer
4 Restriction enzymes - Genetic engineering
Explanation:
Restriction enzyme :- It is a protein (nuclease) that recognize a specific, short nucleotide sequence of DNA. (Known as-restriction site or target sequence or recognition sequence) and then cuts the DNA at that specific site. Restriction enzyme are found in bacteria (and other prokaryotes). Each restriction enzyme, recognizes just one or few restriction site. More than 400 restriction enzymes have been isolated from the bacteria that manufacture them. Central dogma is a theory that show genetic information one direction, from DNA, to RNA, RNA directly to protein Okazaki fragments is found in DNA replication. RNA polymers- synthesize RNA molecular from a template of DNA through a process called transcription.
Punjab MET-2003
Biotechnology : Principles and Processes
171066
Which of the following restriction enzymes produces blunt ends?
1 Sall
2 Eco RV
3 Xho
4 Hind III
Explanation:
Eco RV is a type II restriction endonuclease isolated from E. coli it recognize palindromic sequence and produce blunt ends. $$ \begin{aligned} & 5^{\prime} \mathrm{G}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{C} 3^{\prime} \\ & 3^{\prime} \mathrm{C}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{G} 5^{\prime} \\ & \downarrow \text { Eco RV } \\ & \left.
l
5^ --
3^ --
\right]\left[
l
-- 3^
-- 5
\right. \\ & \end{aligned} $$ Blunt ends
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171067
The most throughly studied of the known bacteria-plant interactions is the :-
1 Gall formation on certain Angiosperms by Agrobacterium
2 Nodulation of Sesbania stems by nitrogen fixing bacteria
3 Plant growth stimulation by phosphatesolubilising bacteria
4 Cyanobacterial symbiosis with some aquatic ferns
Explanation:
Agrobacterium tumefaciens is Rod Shaped, gram negative soil bacterium. - The Agrobacterium Crown Gall phenomenon is extensively used as natural genetic engineer in plant genetic engineering. - It also an example of plant microbe interaction competition commensalism, mutualism and parasitism relationship given by (Campbell 1995). - Agrobacterium has been extensively studied both as a pathogen and an important biotechnological tool. - The infection process involves the transfer of t-DNA and virulence protein into the plant cell.
AIPMT-2004
Biotechnology : Principles and Processes
171071
Match the following enzymes with their functions:
1 A-(ii), B-(iv), C-(i), D-(iii)
2 A-(iii), B-(i), C-(iv), D-(ii)
3 A-(iii), B-(iv), C-(i), D-(ii)
4 A-(iv), B-(iii), C-(i), D-(ii)
Explanation:
Restriction endonuclease - Cuts DNA at specific position. - Restriction endonucleases recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. - Restriction exonuclease -Removes nucleotides From the ends of DNA. Restriction exonucleases remove nucleotides from the terminal ends (either $5^{\prime}$ or 3') of DNA of one strand of duplex. - DNA ligase - Join the DNA fragments. The DNA ligase is functional in joining the Okazaki fragments that take shape on the lagging strand while the DNA replicates. It is able to join the two DNA fragments as a result of the formation of a phosphodiester bond between them with the help of a molecule of energy. - Taq polymerase - Extends primers on genomic DNA template Thermus aquaticus (Taqs) polymerase, exhibits transcriptase activity when a DNA oligomer is used as a template and that genetic information of oligomer can be amplified by PCR using DNA primers.
171091
The figure below is the diagrammatic representation of the E. Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)? E.coli
1 Hindi III, EcoRI-selectable markers
2 ampR, tetR-antibiotic resistance genes
3 ori-original restriction enzyme
4 rop-reduced osmotic pressure
Explanation:
In pBR 322, ori-represents site or origin of replication rop site code for proteins that take part in the replication of plasmid. - ampR and tetR are antibiotic resistance genes. - Hind III, EcoRI- recognition sites of restriction| endonuclease. - EcoRI is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragment at specific sites and also a part of the restriction modification system.
AIPMT (Screening)-2012
Biotechnology : Principles and Processes
171045
Which of the following pairs is correctly matched ?
1 Central dogma - Codon
2 Okazaki fragments - Splicing
3 RNA polymerase - RNA primer
4 Restriction enzymes - Genetic engineering
Explanation:
Restriction enzyme :- It is a protein (nuclease) that recognize a specific, short nucleotide sequence of DNA. (Known as-restriction site or target sequence or recognition sequence) and then cuts the DNA at that specific site. Restriction enzyme are found in bacteria (and other prokaryotes). Each restriction enzyme, recognizes just one or few restriction site. More than 400 restriction enzymes have been isolated from the bacteria that manufacture them. Central dogma is a theory that show genetic information one direction, from DNA, to RNA, RNA directly to protein Okazaki fragments is found in DNA replication. RNA polymers- synthesize RNA molecular from a template of DNA through a process called transcription.
Punjab MET-2003
Biotechnology : Principles and Processes
171066
Which of the following restriction enzymes produces blunt ends?
1 Sall
2 Eco RV
3 Xho
4 Hind III
Explanation:
Eco RV is a type II restriction endonuclease isolated from E. coli it recognize palindromic sequence and produce blunt ends. $$ \begin{aligned} & 5^{\prime} \mathrm{G}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{C} 3^{\prime} \\ & 3^{\prime} \mathrm{C}-\mathrm{T}-\mathrm{A}-\mathrm{T}-\mathrm{A}-\mathrm{G} 5^{\prime} \\ & \downarrow \text { Eco RV } \\ & \left.
l
5^ --
3^ --
\right]\left[
l
-- 3^
-- 5
\right. \\ & \end{aligned} $$ Blunt ends
NEET-2016 Phase-II
Biotechnology : Principles and Processes
171067
The most throughly studied of the known bacteria-plant interactions is the :-
1 Gall formation on certain Angiosperms by Agrobacterium
2 Nodulation of Sesbania stems by nitrogen fixing bacteria
3 Plant growth stimulation by phosphatesolubilising bacteria
4 Cyanobacterial symbiosis with some aquatic ferns
Explanation:
Agrobacterium tumefaciens is Rod Shaped, gram negative soil bacterium. - The Agrobacterium Crown Gall phenomenon is extensively used as natural genetic engineer in plant genetic engineering. - It also an example of plant microbe interaction competition commensalism, mutualism and parasitism relationship given by (Campbell 1995). - Agrobacterium has been extensively studied both as a pathogen and an important biotechnological tool. - The infection process involves the transfer of t-DNA and virulence protein into the plant cell.
AIPMT-2004
Biotechnology : Principles and Processes
171071
Match the following enzymes with their functions:
1 A-(ii), B-(iv), C-(i), D-(iii)
2 A-(iii), B-(i), C-(iv), D-(ii)
3 A-(iii), B-(iv), C-(i), D-(ii)
4 A-(iv), B-(iii), C-(i), D-(ii)
Explanation:
Restriction endonuclease - Cuts DNA at specific position. - Restriction endonucleases recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. - Restriction exonuclease -Removes nucleotides From the ends of DNA. Restriction exonucleases remove nucleotides from the terminal ends (either $5^{\prime}$ or 3') of DNA of one strand of duplex. - DNA ligase - Join the DNA fragments. The DNA ligase is functional in joining the Okazaki fragments that take shape on the lagging strand while the DNA replicates. It is able to join the two DNA fragments as a result of the formation of a phosphodiester bond between them with the help of a molecule of energy. - Taq polymerase - Extends primers on genomic DNA template Thermus aquaticus (Taqs) polymerase, exhibits transcriptase activity when a DNA oligomer is used as a template and that genetic information of oligomer can be amplified by PCR using DNA primers.
