07. DNA Fingerprinting and Human Genome Project
Molecular Basis of Inheritance and

166585 Identify the labels \(M\) and \(N\) in the following Agarose get electrophoresis representation.

1 M- Digested DNA bands
2 M- Hybridised DNA bands
\(\mathrm{N}\) - Hybridised DNA bands
3 M- Largest DNA bands N- Smallest DNA bands
4 M-smallest DNA bands \(\mathrm{N}\)-Largest DNA bands
Molecular Basis of Inheritance and

166566 Match List I with List II.

**List - I** (Discoveries)**List - II** (Scientist)
---------------------------------------------------------------------------------------------
A.PCR technologies1.G. Kohler and C. Milstein
B.DNA fingerprinting2.McClintock
C.Hybridoma technology3.K. Mullis
D.Jumping genes4.Allec Jeffreys
Which one of the following is correctly matched?

1 A-2 B-1 C-4 D-3
2 A-2 B-4 C-1 D-3
3 A-3 B-1 C-4 D-2
4 A-3 B-4 C-1 D-2
Molecular Basis of Inheritance and

166570 Ten E.coli cells with \({ }^{15} \mathrm{~N}\) - dsDNA are incubated in medium containing \({ }^{14} \mathrm{~N}\) nucleotide. After 60 minutes, how many E.coli cells will have DNA totally free from \({ }^{15} \mathrm{~N}\) ?

1 80 cells
2 20 cells
3 40 cells
4 60 cells
Molecular Basis of Inheritance and

166584 Select the correct sequence of steps in DNA fingerprinting involving Southern blot hybridization using radiolabelled VNTR as a probe.
(I) Hybridization using labelled VNTR probe.
(II) Isolation of DNA.
(III) Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon.
(IV) Detection of hybridized DNA fragments by autoradiography.
(V) Separation of DNA fragments by electrophoresis.
(VI) Digestion of DNA by restriction endonucleases.

1 I,V, VI, II, III, and IV
2 II, VI, V, III, I and IV
3 V, I, VI, III, IV and II
4 II, I, V, VI, IV and III
5 II, V, VI, I, III and IV
Molecular Basis of Inheritance and

166557 Name and arrange the following in the order of technique and steps for separation and isolation of DNA fragments
I. DNA fragments moved towards anode under electric field.
II. Exposure to UV radiation.
III. DNA fragments stained with EBr.
IV. Gel electrophoresis.
V. Elution.

1 V II III I IV
2 IV V I III II
3 III II I V IV
4 IV I III II V
Molecular Basis of Inheritance and

166585 Identify the labels \(M\) and \(N\) in the following Agarose get electrophoresis representation.

1 M- Digested DNA bands
2 M- Hybridised DNA bands
\(\mathrm{N}\) - Hybridised DNA bands
3 M- Largest DNA bands N- Smallest DNA bands
4 M-smallest DNA bands \(\mathrm{N}\)-Largest DNA bands
Molecular Basis of Inheritance and

166566 Match List I with List II.

**List - I** (Discoveries)**List - II** (Scientist)
---------------------------------------------------------------------------------------------
A.PCR technologies1.G. Kohler and C. Milstein
B.DNA fingerprinting2.McClintock
C.Hybridoma technology3.K. Mullis
D.Jumping genes4.Allec Jeffreys
Which one of the following is correctly matched?

1 A-2 B-1 C-4 D-3
2 A-2 B-4 C-1 D-3
3 A-3 B-1 C-4 D-2
4 A-3 B-4 C-1 D-2
Molecular Basis of Inheritance and

166570 Ten E.coli cells with \({ }^{15} \mathrm{~N}\) - dsDNA are incubated in medium containing \({ }^{14} \mathrm{~N}\) nucleotide. After 60 minutes, how many E.coli cells will have DNA totally free from \({ }^{15} \mathrm{~N}\) ?

1 80 cells
2 20 cells
3 40 cells
4 60 cells
Molecular Basis of Inheritance and

166584 Select the correct sequence of steps in DNA fingerprinting involving Southern blot hybridization using radiolabelled VNTR as a probe.
(I) Hybridization using labelled VNTR probe.
(II) Isolation of DNA.
(III) Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon.
(IV) Detection of hybridized DNA fragments by autoradiography.
(V) Separation of DNA fragments by electrophoresis.
(VI) Digestion of DNA by restriction endonucleases.

1 I,V, VI, II, III, and IV
2 II, VI, V, III, I and IV
3 V, I, VI, III, IV and II
4 II, I, V, VI, IV and III
5 II, V, VI, I, III and IV
Molecular Basis of Inheritance and

166557 Name and arrange the following in the order of technique and steps for separation and isolation of DNA fragments
I. DNA fragments moved towards anode under electric field.
II. Exposure to UV radiation.
III. DNA fragments stained with EBr.
IV. Gel electrophoresis.
V. Elution.

1 V II III I IV
2 IV V I III II
3 III II I V IV
4 IV I III II V
Molecular Basis of Inheritance and

166585 Identify the labels \(M\) and \(N\) in the following Agarose get electrophoresis representation.

1 M- Digested DNA bands
2 M- Hybridised DNA bands
\(\mathrm{N}\) - Hybridised DNA bands
3 M- Largest DNA bands N- Smallest DNA bands
4 M-smallest DNA bands \(\mathrm{N}\)-Largest DNA bands
Molecular Basis of Inheritance and

166566 Match List I with List II.

**List - I** (Discoveries)**List - II** (Scientist)
---------------------------------------------------------------------------------------------
A.PCR technologies1.G. Kohler and C. Milstein
B.DNA fingerprinting2.McClintock
C.Hybridoma technology3.K. Mullis
D.Jumping genes4.Allec Jeffreys
Which one of the following is correctly matched?

1 A-2 B-1 C-4 D-3
2 A-2 B-4 C-1 D-3
3 A-3 B-1 C-4 D-2
4 A-3 B-4 C-1 D-2
Molecular Basis of Inheritance and

166570 Ten E.coli cells with \({ }^{15} \mathrm{~N}\) - dsDNA are incubated in medium containing \({ }^{14} \mathrm{~N}\) nucleotide. After 60 minutes, how many E.coli cells will have DNA totally free from \({ }^{15} \mathrm{~N}\) ?

1 80 cells
2 20 cells
3 40 cells
4 60 cells
Molecular Basis of Inheritance and

166584 Select the correct sequence of steps in DNA fingerprinting involving Southern blot hybridization using radiolabelled VNTR as a probe.
(I) Hybridization using labelled VNTR probe.
(II) Isolation of DNA.
(III) Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon.
(IV) Detection of hybridized DNA fragments by autoradiography.
(V) Separation of DNA fragments by electrophoresis.
(VI) Digestion of DNA by restriction endonucleases.

1 I,V, VI, II, III, and IV
2 II, VI, V, III, I and IV
3 V, I, VI, III, IV and II
4 II, I, V, VI, IV and III
5 II, V, VI, I, III and IV
Molecular Basis of Inheritance and

166557 Name and arrange the following in the order of technique and steps for separation and isolation of DNA fragments
I. DNA fragments moved towards anode under electric field.
II. Exposure to UV radiation.
III. DNA fragments stained with EBr.
IV. Gel electrophoresis.
V. Elution.

1 V II III I IV
2 IV V I III II
3 III II I V IV
4 IV I III II V
Molecular Basis of Inheritance and

166585 Identify the labels \(M\) and \(N\) in the following Agarose get electrophoresis representation.

1 M- Digested DNA bands
2 M- Hybridised DNA bands
\(\mathrm{N}\) - Hybridised DNA bands
3 M- Largest DNA bands N- Smallest DNA bands
4 M-smallest DNA bands \(\mathrm{N}\)-Largest DNA bands
Molecular Basis of Inheritance and

166566 Match List I with List II.

**List - I** (Discoveries)**List - II** (Scientist)
---------------------------------------------------------------------------------------------
A.PCR technologies1.G. Kohler and C. Milstein
B.DNA fingerprinting2.McClintock
C.Hybridoma technology3.K. Mullis
D.Jumping genes4.Allec Jeffreys
Which one of the following is correctly matched?

1 A-2 B-1 C-4 D-3
2 A-2 B-4 C-1 D-3
3 A-3 B-1 C-4 D-2
4 A-3 B-4 C-1 D-2
Molecular Basis of Inheritance and

166570 Ten E.coli cells with \({ }^{15} \mathrm{~N}\) - dsDNA are incubated in medium containing \({ }^{14} \mathrm{~N}\) nucleotide. After 60 minutes, how many E.coli cells will have DNA totally free from \({ }^{15} \mathrm{~N}\) ?

1 80 cells
2 20 cells
3 40 cells
4 60 cells
Molecular Basis of Inheritance and

166584 Select the correct sequence of steps in DNA fingerprinting involving Southern blot hybridization using radiolabelled VNTR as a probe.
(I) Hybridization using labelled VNTR probe.
(II) Isolation of DNA.
(III) Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon.
(IV) Detection of hybridized DNA fragments by autoradiography.
(V) Separation of DNA fragments by electrophoresis.
(VI) Digestion of DNA by restriction endonucleases.

1 I,V, VI, II, III, and IV
2 II, VI, V, III, I and IV
3 V, I, VI, III, IV and II
4 II, I, V, VI, IV and III
5 II, V, VI, I, III and IV
Molecular Basis of Inheritance and

166557 Name and arrange the following in the order of technique and steps for separation and isolation of DNA fragments
I. DNA fragments moved towards anode under electric field.
II. Exposure to UV radiation.
III. DNA fragments stained with EBr.
IV. Gel electrophoresis.
V. Elution.

1 V II III I IV
2 IV V I III II
3 III II I V IV
4 IV I III II V
Molecular Basis of Inheritance and

166585 Identify the labels \(M\) and \(N\) in the following Agarose get electrophoresis representation.

1 M- Digested DNA bands
2 M- Hybridised DNA bands
\(\mathrm{N}\) - Hybridised DNA bands
3 M- Largest DNA bands N- Smallest DNA bands
4 M-smallest DNA bands \(\mathrm{N}\)-Largest DNA bands
Molecular Basis of Inheritance and

166566 Match List I with List II.

**List - I** (Discoveries)**List - II** (Scientist)
---------------------------------------------------------------------------------------------
A.PCR technologies1.G. Kohler and C. Milstein
B.DNA fingerprinting2.McClintock
C.Hybridoma technology3.K. Mullis
D.Jumping genes4.Allec Jeffreys
Which one of the following is correctly matched?

1 A-2 B-1 C-4 D-3
2 A-2 B-4 C-1 D-3
3 A-3 B-1 C-4 D-2
4 A-3 B-4 C-1 D-2
Molecular Basis of Inheritance and

166570 Ten E.coli cells with \({ }^{15} \mathrm{~N}\) - dsDNA are incubated in medium containing \({ }^{14} \mathrm{~N}\) nucleotide. After 60 minutes, how many E.coli cells will have DNA totally free from \({ }^{15} \mathrm{~N}\) ?

1 80 cells
2 20 cells
3 40 cells
4 60 cells
Molecular Basis of Inheritance and

166584 Select the correct sequence of steps in DNA fingerprinting involving Southern blot hybridization using radiolabelled VNTR as a probe.
(I) Hybridization using labelled VNTR probe.
(II) Isolation of DNA.
(III) Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon.
(IV) Detection of hybridized DNA fragments by autoradiography.
(V) Separation of DNA fragments by electrophoresis.
(VI) Digestion of DNA by restriction endonucleases.

1 I,V, VI, II, III, and IV
2 II, VI, V, III, I and IV
3 V, I, VI, III, IV and II
4 II, I, V, VI, IV and III
5 II, V, VI, I, III and IV
Molecular Basis of Inheritance and

166557 Name and arrange the following in the order of technique and steps for separation and isolation of DNA fragments
I. DNA fragments moved towards anode under electric field.
II. Exposure to UV radiation.
III. DNA fragments stained with EBr.
IV. Gel electrophoresis.
V. Elution.

1 V II III I IV
2 IV V I III II
3 III II I V IV
4 IV I III II V